Geschmack ist vorhersagbar: Mit FlavorMiner. FlavorMiner heißt das Tool, das IPB-Chemiker und Partner aus Kolumbien jüngst entwickelt haben. Das Programm kann, basierend auf maschinellem Lernen (KI), anhand der…
Seit Februar 2021 bietet Wolfgang Brandt, ehemaliger Leiter der Arbeitsgruppe Computerchemie am IPB, sein Citizen Science-Projekt zur Pilzbestimmung an. Dafür hat er in regelmäßigen Abständen öffentliche Vorträge zur Vielfalt…
Lohse, S.; Hause, B.; Hause, G.; Fester, T.;FtsZ Characterization and Immunolocalization in the Two Phases of Plastid Reorganization in Arbuscular Mycorrhizal Roots of Medicago truncatulaPlant Cell Physiol.471124-1134(2006)DOI: 10.1093/pcp/pcj083
We have analyzed plastid proliferation in root cortical cells of Medicago truncatula colonized by arbuscular mycorrhizal (AM) fungi by concomitantly labeling fungal structures, root plastids, a protein involved in plastid division (FtsZ1) and a protein involved in the biosynthesis of AM-specific apocarotenoids. Antibodies directed against FtsZ1 have been generated after heterologous expression of the respective gene from M. truncatula and characterization of the gene product. Analysis of enzymatic activity and assembly experiments showed similar properties of this protein when compared with the bacterial proteins. Immunocytological experiments allowed two phases of fungal and plastid development to be clearly differentiated and plastid division to be monitored during these phases. In the early phase of arbuscule development, lens-shaped plastids, intermingled with the arbuscular branches, divide frequently. Arbuscule degradation, in contrast, is characterized by large, tubular plastids, decorated by a considerable number of FtsZ division rings.
Publikation
Fester, T.; Hause, B.; Schmidt, D.; Halfmann, K.; Schmidt, J.; Wray, V.; Hause, G.; Strack, D.;Occurrence and Localization of Apocarotenoids in Arbuscular Mycorrhizal Plant RootsPlant Cell Physiol.43256-265(2002)DOI: 10.1093/pcp/pcf029
The core structure of the yellow pigment from arbuscular mycorrhizal (AM) maize roots contains the apocarotenoids mycorradicin (an acyclic C14 polyene) and blumenol C cellobioside (a C13 cyclohexenone diglucoside). The pigment seems to be a mixture of different esterification products of these apocarotenoids. It is insoluble in water and accumulates as hydrophobic droplets in the vacuoles of root cortical cells. Screening 58 species from 36 different plant families, we detected mycorradicin in mycorrhizal roots of all Liliopsida analyzed and of a considerable number of Rosopsida, but also species were found in which mycorradicin was undetectable in mycorrhizal roots. Kinetic experiments and microscopic analyses indicate that accumulation of the yellow pigment is correlated with the concomitant degradation of arbuscules and the extensive plastid network covering these haustorium-like fungal structures. The role of the apocarotenoids in mycorrhizal roots is still unknown. The potential C40 carotenoid precursors, however, are more likely to be of functional importance in the development and functioning of arbuscules.