Omanische Heilpflanze im Fokus der Phytochemie IPB-Wissenschaftler und Partner aus Dhofar haben jüngst die omanische Heilpflanze Terminalia dhofarica unter die phytochemische Lupe genommen. Die Pflanze ist reich an…
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The pollen tube is a tip-growing system that delivers sperm to the ovule and thus is essential for sexual plant reproduction. Sucrose and other microelements act as nutrients and signaling molecules through pathways that are not yet fully understood. Taking advantage of high-throughput liquid chromatography coupled to mass spectrometry (LC-MS), we performed a label-free shotgun proteomic analysis of pollen in response to nutrient limitation using mass accuracy precursor alignment. We compared 168 LC-MS analyses and more than 1 million precursor ions and could define the proteomic phenotypes of pollen under different conditions. In total, 166 proteins and 42 phosphoproteins were identified as differentially regulated. These proteins are involved in a variety of signaling pathways, providing new insights into the multifaceted mechanism of nutrient function. The phosphorylation of proteins involved in cytoskeleton dynamics was found to be specifically responsive to Ca2+ and sucrose deficiency, suggesting that sucrose and extracellular Ca2+ influx are necessary for the maintenance of cytoskeleton polymerization. Sucrose limitation leads to widespread accumulation of proteins involved in carbohydrate metabolism and protein degradation. This highlights the wide range of metabolic and cellular processes that are modulated by sucrose but complicates dissection of the signaling pathways.
Publikation
Wienkoop, S.; Staudinger, C.; Hoehenwarter, W.; Weckwerth, W.; Egelhofer, V.;ProMEX – a mass spectral reference database for plant proteomicsFront. Plant Sci.3125(2012)DOI: 10.3389/fpls.2012.00125
The ProMEX database is one of the main collection of annotated tryptic peptides in plant proteomics. The main objective of the ProMEX database is to provide experimental MS/MS-based information for cell type-specific or sub-cellular proteomes in Arabidopsis thaliana, Medicago truncatula, Chlamydomonas reinhardtii, Lotus japonicus, Lotus corniculatus, Phaseolus vulgaris, Lycopersicon esculentum, Solanum tuberosum, Nicotiana tabacum, Glycine max, Zea mays, Bradyrhizobium japonicum, and Sinorhizobium meliloti. Direct links at the protein level to the most relevant databases are present in ProMEX. Furthermore, the spectral sequence information are linked to their respective pathways and can be viewed in pathway maps.