Omanische Heilpflanze im Fokus der Phytochemie IPB-Wissenschaftler und Partner aus Dhofar haben jüngst die omanische Heilpflanze Terminalia dhofarica unter die phytochemische Lupe genommen. Die Pflanze ist reich an…
Geschmack ist vorhersagbar: Mit FlavorMiner. FlavorMiner heißt das Tool, das IPB-Chemiker und Partner aus Kolumbien jüngst entwickelt haben. Das Programm kann, basierend auf maschinellem Lernen (KI), anhand der…
Marillonnet, S.; Grützner, R.;Synthetic DNA Assembly Using Golden Gate Cloning and the Hierarchical Modular Cloning PipelineCurr. Protoc. Mol. Biol.130e115(2020)DOI: 10.1002/cpmb.115
Methods that enable the construction of recombinant DNA molecules are essential tools for biological research and biotechnology. Golden Gate cloning is used for assembly of multiple DNA fragments in a defined linear order in a recipient vector using a one‐pot assembly procedure. Golden Gate cloning is based on the use of a type IIS restriction enzyme for digestion of the DNA fragments and vector. Because restriction sites for the type IIS enzyme used for assembly must be present at the ends of the DNA fragments and vector but absent from all internal sequences, special care must be taken to prepare DNA fragments and the recipient vector with a structure suitable for assembly by Golden Gate cloning. In this article, protocols are presented for preparation of DNA fragments, modules, and vectors suitable for Golden Gate assembly cloning. Additional protocols are presented for assembly of defined parts in a transcription unit, as well as the stitching together of multiple transcription units into multigene constructs by the modular cloning (MoClo) pipeline.
Publikation
Schuster, M.; van der Hoorn, R. A.;Plant biology: distinct new players in processing peptide hormones during abscissionCurr. Biol.30R715-R717(2020)DOI: 10.1016/j.cub.2020.04.072
Flower organ abscission in Arabidopsis is regulated by a peptide hormone that is released from its precursor by a network of redundant subtilases. An exciting new study describes how drought-induced flower abscission in tomato is regulated similarly, but distinctly via a single, different subtilase that releases a very different peptide hormone.