Unser 10. Leibniz Plant Biochemistry Symposium am 7. und 8. Mai war ein großer Erfolg. Thematisch ging es in diesem Jahr um neue Methoden und Forschungsansätze der Naturstoffchemie. Die exzellenten Vorträge über Wirkstoffe…
Omanische Heilpflanze im Fokus der Phytochemie IPB-Wissenschaftler und Partner aus Dhofar haben jüngst die omanische Heilpflanze Terminalia dhofarica unter die phytochemische Lupe genommen. Die Pflanze ist reich an…
Geschmack ist vorhersagbar: Mit FlavorMiner. FlavorMiner heißt das Tool, das IPB-Chemiker und Partner aus Kolumbien jüngst entwickelt haben. Das Programm kann, basierend auf maschinellem Lernen (KI), anhand der…
Cotrim, C. A.; Weidner, A.; Strehmel, N.; Bisol, T. B.; Meyer, D.; Brandt, W.; Wessjohann, L. A.; Stubbs, M. T.;A Distinct Aromatic Prenyltransferase Associated with the Futalosine PathwayChemistrySelect29319-9325(2017)DOI: 10.1002/slct.201702151
Menaquinone (MK) is an electron carrier molecule essential for respiration in most Gram positive bacteria. A crucial step in MK biosynthesis involves the prenylation of an aromatic molecule, catalyzed by integral membrane prenyltransferases of the UbiA (4‐hydroxybenzoate oligoprenyltransferase) superfamily. In the classical MK biosynthetic pathway, the prenyltransferase responsible is MenA (1,4‐dihydroxy‐2‐naphthoate octaprenyltransferase). Recently, an alternative pathway for formation of MK, the so‐called futalosine pathway, has been described in certain micro‐organisms. Until now, five soluble enzymes (MqnA‐MqnE) have been identified in the first steps. In this study, the genes annotated as ubiA from T. thermophilus and S. lividans were cloned, expressed and investigated for prenylation activity. The integral membrane proteins possess neither UbiA nor MenA activity and represent a distinct class of prenyltransferases associated with the futalosine pathway that we term MqnP. We identify a critical residue within a highly conserved Asp‐rich motif that serves to distinguish between members of the UbiA superfamily.
Publikation
Anh, N. T. H.; Sung, T. V.; Wessjohann, L. A.;Further constituents from Ophiopogon japonicusVietnam J. Chem.42261-264(2004)
Anh, N. T. H.; Sung, T. V.; Wessjohann, L. A.; Adam, G.;Some hydroxycinnamic acid esters of phenylethyl alcohol glycosides from Rehmannia glutinosa LiboschVietnam J. Chem.40175-179(2002)