Omanische Heilpflanze im Fokus der Phytochemie IPB-Wissenschaftler und Partner aus Dhofar haben jüngst die omanische Heilpflanze Terminalia dhofarica unter die phytochemische Lupe genommen. Die Pflanze ist reich an…
Geschmack ist vorhersagbar: Mit FlavorMiner. FlavorMiner heißt das Tool, das IPB-Chemiker und Partner aus Kolumbien jüngst entwickelt haben. Das Programm kann, basierend auf maschinellem Lernen (KI), anhand der…
Bette, M.; Rüffer, T.; Bruhn, C.; Schmidt, J.; Steinborn, D.;Synthesis, Characterization, and Reactivity of Diacetylplatinum(II) and -platinum(IV) Complexes Bearing κ2- and κ3-Coordinated Scorpionate LigandsOrganometallics313700-3710(2012)DOI: 10.1021/om3001907
Reactions of the dinuclear platina-β-diketone [Pt2{(COR)2H}2(μ-Cl)2] (1) with K[(pz)3BH] and K[(3,5-Me2pz)3BH] (pz = pyrazolyl; 3,5-Me2pz = 3,5-dimethylpyrazolyl) afforded neutral diacetyl(hydrido)platinum(IV) complexes [Pt(COMe)2H{(pz)3BH}] (4a) and [Pt(COMe)2H{(3,5-Me2pz)3BH}] (4b), bearing κ3-bonded tris(pyrazolyl)borate (scorpionate) ligands. These complexes were found to decompose in chloroform solution under formation of the respective chlorido complexes [Pt(COMe)2Cl{(pz)3BH}] (5a) and [Pt(COMe)2Cl{(3,5-Me2pz)3BH}] (5b) as the initial step. Diacetylplatinum(II) complexes with κ2-coordinated scorpionate ligands (K[Pt(COMe)2{(pz)3BH}], 6a; K[Pt(COMe)2{(3,5-Me2pz)3BH}], 6b; K[Pt(COMe)2{(pz)4B}], 7; K[{Pt(COMe)2}2{(pz)4B}], 8) were obtained in ligand exchange reactions of [Pt(COMe)2(NH2Bn)2] (3; Bn = benzyl) with the respective potassium (pyrazolyl)borates. The deprotonation of the hydrido complexes 4 with potassium methoxide led also to the formation of 6. Diacetylplatinum(II) complexes 6a and 7 were found to react in oxidative addition reactions with alkyl halides to yield diacetylplatinum(IV) complexes of the type [Pt(COMe)2R{(pz)3BH)}] (R = Me, 9a; Et, 9b; Bn, 9c) and [Pt(COMe)2R{(pz)4B}] (R = Me, 10a; Et, 10b; Bn, 10c), respectively, with κ3-bonded scorpionate ligands. The identities of all platinum complexes were unambiguously proved by microanalyses or by high-resolution mass spectrometric investigations, by NMR (1H, 13C, 195Pt) and IR spectroscopies, and by single-crystal X-ray diffraction analyses (4a, 5a, 7·(18C6), 9c; 18C6 = 18-crown-6). The reactivity of the complexes is discussed in terms of hemilability of the scorpionate ligands.
Publikation
Werner, S.; Engler, C.; Weber, E.; Gruetzner, R.; Marillonnet, S.;Fast track assembly of multigene constructs using Golden Gate cloning and the MoClo systemBioengineered338-43(2012)DOI: 10.4161/bbug.3.1.18223
Recent progress in the field of synthetic biology has led to the creation of cells containing synthetic genomes. Although these first synthetic organisms contained copies of natural genomes, future work will be directed toward engineering of organisms with modified genomes and novel phenotypes. Much work, however, remains to be done to be able to routinely engineer novel biological functions. As a tool that will be useful for such purpose, we have recently developed a modular cloning system (MoClo) that allows high throughput assembly of multiple genetic elements. We present here new features of this cloning system that allow to increase the speed of assembly of multigene constructs. As an example, 68 DNA fragments encoding basic genetic elements were assembled using three one-pot cloning steps, resulting in a 50 kb construct containing 17 eukaryotic transcription units. This cloning system should be useful for generating the multiple construct variants that will be required for developing gene networks encoding novel functions, and fine-tuning the expression levels of the various genes involved.