Omanische Heilpflanze im Fokus der Phytochemie IPB-Wissenschaftler und Partner aus Dhofar haben jüngst die omanische Heilpflanze Terminalia dhofarica unter die phytochemische Lupe genommen. Die Pflanze ist reich an…
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The pollen tube is a tip-growing system that delivers sperm to the ovule and thus is essential for sexual plant reproduction. Sucrose and other microelements act as nutrients and signaling molecules through pathways that are not yet fully understood. Taking advantage of high-throughput liquid chromatography coupled to mass spectrometry (LC-MS), we performed a label-free shotgun proteomic analysis of pollen in response to nutrient limitation using mass accuracy precursor alignment. We compared 168 LC-MS analyses and more than 1 million precursor ions and could define the proteomic phenotypes of pollen under different conditions. In total, 166 proteins and 42 phosphoproteins were identified as differentially regulated. These proteins are involved in a variety of signaling pathways, providing new insights into the multifaceted mechanism of nutrient function. The phosphorylation of proteins involved in cytoskeleton dynamics was found to be specifically responsive to Ca2+ and sucrose deficiency, suggesting that sucrose and extracellular Ca2+ influx are necessary for the maintenance of cytoskeleton polymerization. Sucrose limitation leads to widespread accumulation of proteins involved in carbohydrate metabolism and protein degradation. This highlights the wide range of metabolic and cellular processes that are modulated by sucrose but complicates dissection of the signaling pathways.
Publikation
Yeboah, S. O.; Mitei, Y. C.; Ngila, J. C.; Wessjohann, L.; Schmidt, J.;Compositional and structural studies of the oils from two edible seeds: Tiger nut, Cyperus esculentum, and asiato, Pachira insignis, from GhanaFood Res. Int.47259-266(2012)DOI: 10.1016/j.foodres.2011.06.036
A comprehensive lipid profiling has been carried out on the seed oils of tiger nut, Cyperus esculentum, and asiato, Pachira insignis, from Ghana in order to evaluate their potential uses for the region. Composition of FAs and phytosterols were determined using GC–MS, while tocol composition was determined with HPLC. TAG composition and regiochemistry were determined using ES-FTICR-MS and 13C NMR, respectively. The major FA components in asiato seed oil were palmitic acid (56.58%) and sterculic and dihydrosterculic acids (20.06% combined). The major FA components in tiger nut oil were oleic (65.55%), palmitic (16. 32%), and linoleic (12.13%) acids. Asiato seed oil had 18 major TAG classes, dominated by, C51:1 (38.45%) and C50:1 (13.941%). Tiger nut oil had 7 major TAG classes, with C54:3 (29.00%) and C52:2 (27.82%) dominating. The sn-1/3 and sn-2 positions in the TAGS for asiato oil were predominantly occupied by saturated acyl chains (87.25%) and cyclic acyl chains (71.44%), respectively. Oleoyl chain primarily occupied both sn-1/3 (52.68%) and sn-2 (77.62%) positions in the tiger nut oil. Total tocol content in asiato oil was 200.31 μg/g, with γ-tocopherol (182.99 μg/g) dominating. Tiger nut oil had a total tocol content of 120.10 μg/g, dominated by α-tocopherol (86.73) and β-tocopherol (33.37 μg/g).Tiger nut oil had total 4-desmethylsterol content of 986 μg/g, dominated by β-sitosterol (517.25 μg/g) and stigmasterol (225.25 μg/g), while asiato oil had total desmethylsterol content of 590.24 μg/g, dominated by β-sitosterol (518.91 μg/g). The presence of cyclopropenoid FAs in asiato oil makes it unsuitable for food uses. Tiger nut oil can replace imported olive oil in food products in the West African region.