Omanische Heilpflanze im Fokus der Phytochemie IPB-Wissenschaftler und Partner aus Dhofar haben jüngst die omanische Heilpflanze Terminalia dhofarica unter die phytochemische Lupe genommen. Die Pflanze ist reich an…
Geschmack ist vorhersagbar: Mit FlavorMiner. FlavorMiner heißt das Tool, das IPB-Chemiker und Partner aus Kolumbien jüngst entwickelt haben. Das Programm kann, basierend auf maschinellem Lernen (KI), anhand der…
Heuts, D. P. H. M.; Weissenborn, M. J.; Olkhov, R. V.; Shaw, A. M.; Gummadova, J.; Levy, C.; Scrutton, N. S.;Crystal Structure of a Soluble Form of Human CD73 with Ecto-5′-Nucleotidase ActivityChemBioChem132384-2391(2012)DOI: 10.1002/cbic.201200426
CD73 is a dimeric ecto‐5′‐nucleotidase that is expressed on the exterior side of the plasma membrane. CD73 has important regulatory functions in the extracellular metabolism of certain nucleoside monophosphates, in particular adenosine monophosphate, and has been linked to a number of pathological conditions such as cancer and myocardial ischaemia. Here, we present the crystal structure of a soluble form of human soluble CD73 (sCD73) at 2.2 Å resolution, a truncated form of CD73 that retains ecto‐5′‐nucleotidase activity. With this structure we obtained insight into the dimerisation of CD73, active site architecture, and a sense of secondary modifications of the protein. The crystal structure reveals a conserved loop that is directly involved in the dimer‐dimer interaction showing that the two subunits of the dimer are not linked by disulfide bridges. Using biophotonic microarray imaging we were able to confirm glycosylation of the enzyme and show that the enzyme is decorated with a variety of oligosaccharide structures. The crystal structure of sCD73 will aid the design of inhibitors or activator molecules for the treatment of several diseases and prove useful in explaining the possible roles of single nucleotide polymorphisms in physiology and disease.
Publikation
Arnold, N.; Palfner, G.; Schmidt, J.; Kuhnt, C.; Becerra, J.;CHEMISTRY OF THE AROMA BOUQUET OF THE EDIBLE MUSHROOM "LEBRE" (Cortinarius lebre, BASDIOMYCOTA, AGARICALES) FROM CHILEJ. Chil. Chem. Soc.571333-1335(2012)DOI: 10.4067/S0717-97072012000300029
Cortinarius lebre Garrido, a common edible mushroom from central Chile, is distinct from other Cortinarii by its strong, naphtalene-like smell. The relevant volatile compounds were detected by gas chromatography - mass spectrometry and identified as indole together with 1-octen-3-ol, octane-3-one, and octan-3-ol.