IPB Halle: Leibniz-Institut für Pflanzenbiochemie Deutsch

Lange Nacht, die Wissen schafft

4. Juli 2025 am IPB
IPB-Newsletter | April 2025
Wir verstehen Pflanzen!

Der Film zum Institut
Molekulare Signalverarbeitung

Prof. Dr. Steffen Abel
Wie Pflanzen ihrer Umwelt trotzen.
Natur- und Wirkstoffchemie

Prof. Dr. Ludger Wessjohann
Die Chemie der Natur – Basis für Wirkstoffe zur Gesunderhaltung von Mensch und Pflanze.
Biochemie pflanzlicher Interaktionen

Prof. Dr. Tina Romeis
Wechselwirkungen in, von und mit Pflanzen.
Stoffwechsel- und Zellbiologie

Prof. Dr. Alain Tissier
Sekundär jedoch nicht zweitrangig - Die biologischen Funktionen pflanzlicher Sekundärmetaboliten.
Unabhängige Nachwuchsgruppe

Dr. Mariana Schuster

Aktuelles

Unser 10. Leibniz Plant Biochemistry Symposium am 7. und 8. Mai war ein großer Erfolg. Thematisch ging es in diesem Jahr um neue Methoden und Forschungsansätze der Naturstoffchemie. Die exzellenten Vorträge über Wirkstoffe…

Omanische Heilpflanze im Fokus der Phytochemie
IPB-Wissenschaftler und Partner aus Dhofar haben jüngst die omanische Heilpflanze Terminalia dhofarica unter die phytochemische Lupe genommen. Die Pflanze ist reich an…

Geschmack ist vorhersagbar: Mit FlavorMiner.
FlavorMiner heißt das Tool, das IPB-Chemiker und Partner aus Kolumbien jüngst entwickelt haben. Das Programm kann, basierend auf maschinellem Lernen (KI), anhand der…

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Referent*in der Geschäftsführung (m/w/d) (10/2025)

Datum: 14.05.2025 Referenznummer: 10/2025

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Farag, M. A.; Saied, D. B.; Afifi, S. M.; Kunzmann, A.; Wessjohann, L. A.; Westphal, H.; Kühnhold, H.; Stuhr, M.; Metabolic responses of sea anemone and jellyfish to temperature and UV bleaching: Insights into stress adaptation using LCMS-based metabolomics, molecular networking and chemometrics J. Adv. Res. (2024) DOI: 10.1016/j.jare.2024.10.007

Introduction: Climate change poses various threats to marine life, particularly in shallow tropical waters. Objective: The impact of increased temperature and ultraviolet (UV) exposure on two photosymbiotic cnidarians, a common bubble-tip anemone and an upside-down jellyfish, was investigated. Methods: To illustrate the response of aquatic organisms, the metabolomes of unstressed Entacmaea quadricolor and Cassiopea andromeda were compared for detailed metabolite profiling. UHPLC-MS cou-pled with chemometrics and GNPS molecular networking was employed for sample classification and identification of markers unique to stress responses in each organism. Results: Several compounds with bioactive functions, including peptides and terpenoids, were reported for the first time in both organisms, viz. cyclic tetraglutamate, campestriene, and ceramide aminoethyl phosphonate (CEAP d18:2/16:0). Both anemone and jellyfish were subjected to either elevated UV-B light intensity up to 6.6 KJ m-2 or increased temperatures (28°C, 30°C, 32°C, and 34°C) over 4 days. Phospholipids, steroids, and ceramides emerged as chief markers of both types of stress, as revealed by the multivariate data analysis. Lysophosphatidylcholine (LPC 16:0), LPC (18:0/0:0), and echinoclasterol sulfate appeared as markers in both UV and thermal stress models of the anemone, whereas methyl/pro-pyl cholestane-hexa-ol were discriminatory in the UV stress model only. In the case of jellyfish, nonpolar glycosyl ceramide GlcCer (d14:1/28:6) served as a marker for UV stress, whereas polar peptides were ele-vated in the thermal stress model. Interestingly, both models of jellyfish share a phospholipid, lysophos-phatidylethanolamine (LPE 20:4), as a distinctive marker for stress, reported to be associated indirectly with the activity of innate immune response within other photosymbiotic Cnidaria such as corals and appears to be a fundamental stress response in marine organisms. Conclusion: This study presents several bioinformatic tools for the first time in two cnidarian organisms to provide not only a broader coverage of their metabolome but also broader insights into cnidarian bleaching in response to different stressors, i.e., heat and UV light, by comparing their effects in anemone versus jellyfish.

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Farag, M. A.; Abdelwareth, A.; Sallam, I. E.; el Shorbagi, M.; Jehmlich, N.; Fritz-Wallace, K.; Serena Schäpe, S.; Rolle-Kampczyk, U.; Ehrlich, A.; Wessjohann, L. A.; von Bergen, M.; Metabolomics reveals impact of seven functional foods on metabolic pathways in a gut microbiota model J. Adv. Res. 23 47-59 (2020) DOI: 10.1016/j.jare.2020.01.001

Functional food defined as dietary supplements that in addition to their nutritional values, can beneficially modulate body functions becomes more and more popular but the reaction of the intestinal microbiota to it is largely unknown. In order to analyse the impact of functional food on the microbiota itself it is necessary to focus on the physiology of the microbiota, which can be assessed in a whole by untargeted metabolomics. Obtaining a detailed description of the gut microbiota reaction to food ingredients can be a key to understand how these organisms regulate and bioprocess many of these food components. Extracts prepared from seven chief functional foods, namely green tea, black tea, Opuntia ficus-indica (prickly pear, cactus pear), black coffee, green coffee, pomegranate, and sumac were administered to a gut consortium culture encompassing 8 microbes which are resembling, to a large extent, the metabolic activities found in the human gut. Samples were harvested at 0.5 and 24 h post addition of functional food extract and from blank culture in parallel and analysed for its metabolites composition using gas chromatography coupled to mass spectrometry detection (GC-MS). A total of 131 metabolites were identified belonging to organic acids, alcohols, amino acids, fatty acids, inorganic compounds, nitrogenous compounds, nucleic acids, phenolics, steroids and sugars, with amino acids as the most abundant class in cultures. Considering the complexity of such datasets, multivariate data analyses were employed to classify samples and investigate how functional foods influence gut microbiota metabolisms. Results from this study provided a first insights regarding how functional foods alter gut metabolism through either induction or inhibition of certain metabolic pathways, i.e. GABA production in the presence of higher acidity induced by functional food metabolites such as polyphenols. Likewise, functional food metabolites i.e., purine alkaloids acted themselves as direct substrate in microbiota metabolism.

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Farag, M. A.; Porzel, A.; Wessjohann, L. A.; Unequivocal glycyrrhizin isomer determination and comparative in vitro bioactivities of root extracts in four Glycyrrhiza species J. Adv. Res. 6 99-104 (2015) DOI: 10.1016/j.jare.2014.05.001

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Farag, M. A.; Wessjohann, L. A.; Cytotoxic effect of commercial Humulus lupulus L. (hop) preparations – In comparison to its metabolomic fingerprint J. Adv. Res. 4 417-421 (2013) DOI: 10.1016/j.jare.2012.07.006

Mehr erhalten

Lange Nacht, die Wissen schafft

IPB-Newsletter | April 2025

Wir verstehen Pflanzen!

Molekulare Signalverarbeitung

Natur- und Wirkstoffchemie

Biochemie pflanzlicher Interaktionen

Stoffwechsel- und Zellbiologie

Unabhängige Nachwuchsgruppe

Aktuelles

Gelungenes Symposium

+++ Newsticker Wissenschaft #182 +++ Naturstoffe +++

+++ Newsticker Wissenschaft #181 +++ Maschinelles Lernen +++

Stellenausschreibungen

Referent*in der Geschäftsführung (m/w/d) (10/2025)

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