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Lange Nacht, die Wissen schafft

4. Juli 2025 am IPB
IPB-Newsletter | April 2025
Wir verstehen Pflanzen!

Der Film zum Institut
Molekulare Signalverarbeitung

Prof. Dr. Steffen Abel
Wie Pflanzen ihrer Umwelt trotzen.
Natur- und Wirkstoffchemie

Prof. Dr. Ludger Wessjohann
Die Chemie der Natur – Basis für Wirkstoffe zur Gesunderhaltung von Mensch und Pflanze.
Biochemie pflanzlicher Interaktionen

Prof. Dr. Tina Romeis
Wechselwirkungen in, von und mit Pflanzen.
Stoffwechsel- und Zellbiologie

Prof. Dr. Alain Tissier
Sekundär jedoch nicht zweitrangig - Die biologischen Funktionen pflanzlicher Sekundärmetaboliten.
Unabhängige Nachwuchsgruppe

Dr. Mariana Schuster

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FlavorMiner heißt das Tool, das IPB-Chemiker und Partner aus Kolumbien jüngst entwickelt haben. Das Programm kann, basierend auf maschinellem Lernen (KI), anhand der…

Im Rahmen der Wirkstofftage am 9. April 2025 verlieh das Leibniz-Forschungsnetzwerk Wirkstoffe den Preis „Leibniz-Wirkstoff des Jahres“ 2025 an Dr. Ibrahim Morgan, Dr. Robert Rennert, Dr. Tuvshinjargal Budragchaa und Prof.…

Seit Februar 2021 bietet Wolfgang Brandt, ehemaliger Leiter der Arbeitsgruppe Computerchemie am IPB, sein Citizen Science-Projekt zur Pilzbestimmung an. Dafür hat er in regelmäßigen Abständen öffentliche Vorträge zur Vielfalt…

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Laborantin bzw. Technischer Assistent*in (m/w/d) (9/2025)

Datum: 08.04.2025 Referenznummer: 9/2025

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Publikation

Baky, M. H.; Kamal, I. M.; Wessjohann, L. A.; Farag, M. A.; Assessment of metabolome diversity in black and white pepper in response to autoclaving using MS- and NMR-based metabolomics and in relation to its remote and direct antimicrobial effects against food-borne pathogens RSC Adv. 14 10799-10813 (2024) DOI: 10.1039/d4ra00100a

Publikation

Farag, M. A.; Baky, M. H.; Morgan, I.; Khalifa, M. R.; Rennert, R.; Mohamed, O. G.; El-Sayed, M. M.; Porzel, A.; Wessjohann, L. A.; Ramadan, N. S.; Comparison of Balanites aegyptiaca parts: metabolome providing insights into plant health benefits and valorization purposes as analyzed using multiplex GC-MS, LC-MS, NMR-based metabolomics, and molecular networking RSC Adv. 13 21471-21493 (2023) DOI: 10.1039/d3ra03141a

Balanites aegyptiaca (L.) Delile (Zygophyllaceae), also known as the desert date, is an edible fruit-producing tree popular for its nutritional and several health benefits. In this study, multi-targeted comparative metabolic profiling and fingerprinting approaches were conducted for the assessment of the nutrient primary and secondary metabolite heterogeneity in different parts, such as leaves, stems, seeds, unripe, and ripe fruits of B. aegyptiaca using nuclear magnetic resonance (NMR), ultra-performance liquid chromatography (UPLC-MS), and gas chromatography mass-spectrometry (GC-MS) based metabolomics coupled to multivariate analyses and in relation to its cytotoxic activities. NMR-based metabolomic study identified and quantified 15 major primary and secondary metabolites belonging to alkaloids, saponins, flavonoids, sugars, and amino and fatty acids. Principal component analysis (PCA) of the NMR dataset revealed α-glucose, sucrose, and isorhamnetin as markers for fruit and stem and unsaturated fatty acids for predominated seeds. Orthogonal projections to latent structure discriminant analysis (OPLS-DA) revealed trigonelline as a major distinctive metabolite in the immature fruit and isorhamnetin as a major distinct marker in the mature fruit. UPLC-MS/MS analysis using feature-based molecular networks revealed diverse chemical classes viz. steroidal saponins, N-containing metabolites, phenolics, fatty acids, and lipids as the constitutive metabolome in Balanites. Gas chromatography-mass spectroscopy (GC-MS) profiling of primary metabolites led to the detection of 135 peaks belonging to sugars, fatty acids/esters, amino acids, nitrogenous, and organic acids. Monosaccharides were detected at much higher levels in ripe fruit and disaccharides in predominate unripe fruits, whereas B. aegyptiaca vegetative parts (leaves and stem) were rich in amino acids and fatty acids. The antidiabetic compounds, viz, nicotinic acid, and trigonelline, were detected in all parts especially unripe fruit in addition to the sugar alcohol D-pinitol for the first time providing novel evidence for B. aegyptiaca use in diabetes. In vitro cytotoxic activity revealed the potential efficacy of immature fruit and seeds as cytotoxic agents against human prostate cancer (PC3) and human colorectal cancer (HCT-116) cell lines. Collectively, such detailed profiling of parts provides novel evidence for B. aegyptiaca medicinal uses.

Publikation

Blatt-Janmaat, K.; Neumann, S.; Schmidt, F.; Ziegler, J.; Qu, Y.; Peters, K.; Impact of in vitro phytohormone treatments on the metabolome of the leafy liverwort Radula complanata (L.) Dumort Metabolomics 19 17 (2023) DOI: 10.1007/s11306-023-01979-y

Introduction Liverworts are a group of non-vascular plants that possess unique metabolism not found in other plants. Many liverwort metabolites have interesting structural and biochemical characteristics, however the fluctuations of these metabolites in response to stressors is largely unknown. Objectives To investigate the metabolic stress-response of the leafy liverwort Radula complanata. Methods Five phytohormones were applied exogenously to in vitro cultured R. complanata and an untargeted metabolomic analysis was conducted. Compound classification and identification was performed with CANOPUS and SIRIUS while statistical analyses including PCA, ANOVA, and variable selection using BORUTA were conducted to identify metabolic shifts.Results It was found that R. complanata was predominantly composed of carboxylic acids and derivatives, followed by benzene and substituted derivatives, fatty acyls, organooxygen compounds, prenol lipids, and flavonoids. The PCA revealed that samples grouped based on the type of hormone applied, and the variable selection using BORUTA (Random Forest) revealed 71 identified and/or classified features that fluctuated with phytohormone application. The stress-response treatments largely reduced the production of the selected primary metabolites while the growth treatments resulted in increased production of these compounds. 4-(3-Methyl-2-butenyl)-5-phenethylbenzene-1,3-diol was identified as a biomarker for the growth treatments while GDP-hexose was identified as a biomarker for the stress-response treatments. Conclusion Exogenous phytohormone application caused clear metabolic shifts in Radula complanata that deviate from the responses of vascular plants. Further identification of the selected metabolite features can reveal metabolic biomarkers unique to liverworts and provide more insight into liverwort stress responses.

Publikation

Serag, A.; Baky, M. H.; Döll, S.; Farag, M. A.; UHPLC-MS metabolome based classification of umbelliferous fruit taxa: a prospect for phyto-equivalency of its different accessions and in response to roasting RSC Adv. 10 76-85 (2020) DOI: 10.1039/C9RA07841J

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Lange Nacht, die Wissen schafft

IPB-Newsletter | April 2025

Wir verstehen Pflanzen!

Molekulare Signalverarbeitung

Natur- und Wirkstoffchemie

Biochemie pflanzlicher Interaktionen

Stoffwechsel- und Zellbiologie

Unabhängige Nachwuchsgruppe

Aktuelles

+++ Newsticker Wissenschaft #181 +++ Maschinelles Lernen +++

Preisverleihung zu den Wirkstofftagen: “Leibniz-Wirkstoff des Jahres 2025” geht an IPB-Team für Krebsforschungsprojekt

Pilzsachverständige in Sachsen-Anhalt: Jubiläumsstimmung zur Frühjahrstagung

Stellenausschreibungen

Laborantin bzw. Technischer Assistent*in (m/w/d) (9/2025)

Publikationen

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