Suchfilter
- Erscheinungsjahr
-
2005 (1)
-
- Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert
-
Plant J. (8)J. Biol. Chem. (4)PLOS ONE (4)0 (2)Amino Acids (2)BMC Plant Biol. (2)Curr. Biol. (2)Phytochemistry (2)Plant Physiol. (2)Anal. Biochem. (1)BMC Biol. (1)Botany (1)Cell Rep. (1)Equine Vet. J. (1)J. Biotechnol. (1)J. Chromatogr. A (1)J. Exp. Bot. (1)Metabolomics (1)Mol. Plant (1)Nat. Plants (1)Pharmazie (1)Plant Mol. Biol. (1)Planta (1)Plants (1)Sci. Rep. (1)Toxins (1)bioRxiv (1)eLife (1)
-
- Autor Nach Häufigkeit alphabetisch sortiert
-
Strack, D. (18)Hause, B. (12)Wasternack, C. (8)Milkowski, C. (6)Feussner, I. (5)Vogt, T. (5)Scheel, D. (4)Walter, M. H. (4)Clemens, S. (3)Fester, T. (3)Kutchan, T. M. (3)Schmidt, J. (3)Atzorn, R. (2)Böhm, H. (2)Delker, C. (2)Dräger, B. (2)Fellenberg, C. (2)Fischer, K. (2)Kindl, H. (2)Kramell, R. (2)Maier, W. (2)Miersch, O. (2)Mittasch, J. (2)Parthier, B. (2)Rosahl, S. (2)Weichert, H. (2)Wray, V. (2)Abel, S. (1)Albert, A. (1)Ammer, C. (1)Apel, K. (1)Bakker, A. (1)Baumert, A. (1)Beekwilder, J. (1)Bektas, I. (1)Birschwilks, M. (1)Bisseling, T. (1)Bloß, T. (1)Brabant, A. (1)Bramley, P. M. (1)Brückner, C. (1)Böttcher, C. (1)Chapple, C. (1)Chen, D. L. (1)Christ, R. (1)Dammann, U. (1)De Lange, E. S. (1)De-Eknamkul, W. (1)Deepak, S. (1)Delatorre, C. A. (1)Dunaeva, M. (1)Floss, D. S. (1)Fodor, J. (1)Franken, P. (1)Fraser, P. D. (1)Frenzel, A. (1)Funke, E. (1)Gao, X. (1)Gebhardt, C. (1)Gerhardt, B. (1)Gesell, A. (1)Giuliano, G. (1)Gresshoff, P. (1)Grimm, R. (1)Groß, N. (1)Grunwald, U. (1)Guex, N. (1)Guo, W. (1)Görschen, E. (1)Halim, V. A. (1)Hammer, K. (1)Handrick, V. (1)Hans, J. (1)Hause, G. (1)Heuer, S. (1)Hilpert, B. (1)Hohlfeld, H. (1)Höhne, M. (1)Hückelhoven, R. (1)Isayenkov, S. (1)Jones, P. (1)Kaiser, H. (1)Keiner, R. (1)Kini, R. K. (1)Kogel, K.-H. (1)Kolbe, A. (1)Kolomiets, M. (1)Krajinski, F. (1)Kraus, A. (1)Köck, M. (1)Kühn, H. (1)Küllertz, G. (1)Küster, H. (1)Küster, N. (1)Lehmann, J. (1)Ziegler, J. (0)
-
- Ergebnisse als:
- Druckansicht
- Endnote (RIS)
- BibTeX
- Tabelle: CSV | HTML
Publikation
Benzylisoquinoline alkaloids constitute a group of about 2,500 structures and are mainly produced by plants of the order Ranunculales. But only the opium poppy, Papaver somniferum, and Papaver setigerum are able to produce morphine. In this study, we started to investigate by gene expression analysis the molecular basis for this exceptional biosynthetic ability. A sequencing project from P. somniferum seedlings was initiated using a method based on the amplified fragment length polymorphism technique that resulted in 849 UniGenes. These cDNAs were analysed on macroarrays for differential expression between morphine-containing P. somniferum plants and eight other Papaver species, which accumulate other benzylisoquinolines instead of morphine. Three cDNAs showing increased expression in P. somniferum compared to all the other Papaver species were identified. Whereas two showed no significant homology to any known protein, one putatively encoded an O-methyltransferase. Analysis of substrate specificity of the heterologously expressed protein and mass spectrometric identification of the enzymatic products identified this protein as S-adenosyl-L-methionine:(R,S)-3′-hydroxy-N-methylcoclaurine 4′-O-methyltransferase (EC 2.1.1.116). Unlike other O-methyltransferases of different positional specificities implicated in benzylisoquinoline metabolism, the enzyme only accepted tetrahydroxylated tetrahydrobenzylisoquinolines as substrates; methylation was tolerated only at the 6-hydroxy position.