Dem IPB wird erneut ein beispielhaftes Handeln im Sinne einer chancengleichheitsorientierten Personal- und Organisationspolitik bescheinigt. Das Institut erhält zum 6. Mal in Folge das TOTAL E-QUALITY…
Die Plant Science Student Conference (PSSC) wird seit 20 Jahren im jährlichen Wechsel von Studierenden der beiden Leibniz-Institute IPK und IPB organisiert. Im Interview erläutern Christina Wäsch…
Sultani, H. N.; Morgan, I. M.; Hussain, H.; Haeri, H. H.; Hinderberger, D.; Kaluđerović, G. N.; Westermann, B.;Synthesis of rhodamine TEMPO conjugates via isonitrile‐based multicomponent‐reactions for mitochondria‐targeted ROS‐detection in cancer cellsAdvanced Sensor Research42400180(2025)DOI: 10.1002/adsr.202400180
A novel series of profluorescent rhodamine nitroxide conjugates are synthesized utilizing well‐known isonitrile‐based multicomponent reactions (IMCRs). The synthesized conjugates are rationally designed as mitochondria‐targeting probes for the detection of reactive oxygen species in living cells. Herein, the synthesized probes demonstrate high selectivity to target the mitochondria of both of PC3‐ and NIH3T3‐cells which represent cancer and normal cell lines. Attaching TEMPO nitroxide to rhodamine leads to fluorescence quenching, allowing for ROS detection and quantification. The prepared sensors provide a reliable method for distinguishing between different oxidative environments in living organisms through different levels of fluorescence to be measured. The use of the Ugi multicomponent reaction enables an efficient and versatile synthetic approach, offering significant advantages over previously reported methods for constructing ROS‐detecting probes. The simplicity of the reaction setup and the ability to generate a diverse library of products by varying Ugi components make this protocol highly adaptable for further chemical modification and potential applications in biological systems.
Publikation
Panda, S.; Chappell-Maor, L.; Alejandro de Haro, L.; Jozwiak, A.; Gharat, S. A.; Kazachkova, Y.; Cai, J.; Vainer, A.; Toppino, L.; Sehrawat, U.; Wizler, G.; Pliner, M.; Meir, S.; Rotino, G. L.; Yasuor, H.; Rogachev, I.; Aharoni, A.;Molecular mechanisms driving the unusual pigmentation shift during eggplant fruit developmentPlant Communications6101321(2025)DOI: 10.1016/j.xplc.2025.101321
Fruit pigmentation is a major signal that attracts frugivores to enable seed dispersal. In most fleshy fruit, green chlorophyll typically accumulates early in development and is replaced by a range of pigments during ripening. In species such as grape and strawberry, chlorophyll is replaced by red anthocyanins produced by the flavonoid biosynthetic pathway. Eggplant (Solanum melongena) is unique, as its fruit accumulates anthocyanins beginning from fruit set, and these are later replaced by the yellow flavonoid-pathway intermediate naringenin chalcone. To decipher the genetic regulation of this extraordinary pigmentation shift, we integrated mRNA and microRNA (miRNA) profiling data obtained from developing eggplant fruit. We discovered that SQUAMOSA PROMOTER BINDING-LIKE (i.e., SPL6a, SPL10, and SPL15), MYB1, and MYB2 transcription factors (TFs) regulate anthocyanin biosynthesis in early fruit development, whereas the MYB12 TF controls later accumulation of naringenin chalcone. We further show that miRNA157 and miRNA858 negatively regulate the expression of SPLs and MYB12, respectively. Taken together, our findings suggest that opposing and complementary expression of miRNAs and TFs controls the pigmentation switch in eggplant fruit skin. Intriguingly, despite the distinctive pigmentation pattern in eggplant, fruit development in other species makes use of homologous regulatory factors to control the temporal and spatial production of different pigment classes.