Dem IPB wird erneut ein beispielhaftes Handeln im Sinne einer chancengleichheitsorientierten Personal- und Organisationspolitik bescheinigt. Das Institut erhält zum 6. Mal in Folge das TOTAL E-QUALITY…
Die Plant Science Student Conference (PSSC) wird seit 20 Jahren im jährlichen Wechsel von Studierenden der beiden Leibniz-Institute IPK und IPB organisiert. Im Interview erläutern Christina Wäsch…
Sultani, H. N.; Morgan, I. M.; Hussain, H.; Haeri, H. H.; Hinderberger, D.; Kaluđerović, G. N.; Westermann, B.;Synthesis of rhodamine TEMPO conjugates via isonitrile‐based multicomponent‐reactions for mitochondria‐targeted ROS‐detection in cancer cellsAdvanced Sensor Research42400180(2025)DOI: 10.1002/adsr.202400180
A novel series of profluorescent rhodamine nitroxide conjugates are synthesized utilizing well‐known isonitrile‐based multicomponent reactions (IMCRs). The synthesized conjugates are rationally designed as mitochondria‐targeting probes for the detection of reactive oxygen species in living cells. Herein, the synthesized probes demonstrate high selectivity to target the mitochondria of both of PC3‐ and NIH3T3‐cells which represent cancer and normal cell lines. Attaching TEMPO nitroxide to rhodamine leads to fluorescence quenching, allowing for ROS detection and quantification. The prepared sensors provide a reliable method for distinguishing between different oxidative environments in living organisms through different levels of fluorescence to be measured. The use of the Ugi multicomponent reaction enables an efficient and versatile synthetic approach, offering significant advantages over previously reported methods for constructing ROS‐detecting probes. The simplicity of the reaction setup and the ability to generate a diverse library of products by varying Ugi components make this protocol highly adaptable for further chemical modification and potential applications in biological systems.
Publikation
Otify, A. M.; Serag, A.; Porzel, A.; Wessjohann, L. A.; Farag, M. A.;NMR metabolome-based classification of Cymbopogon Species: a prospect for phyto-equivalency of its different accessions using chemometric toolsFood Analytical Methods152095-2106(2022)DOI: 10.1007/s12161-022-02257-8
Cymbopogon species are widely distributed worldwide and known for their high essential oil content with potential commercial and medicinal benefits justifying for their inclusion in food and cosmetics. Most species received scant characterization regarding their full complement of bioactive constituents necessary to explain their medicinal activities. In this study, the metabolite profiles of 5 Cymbopogon species, C. citratus, C. flexuosus, C. procerus, C. martini, and C. nardus, were characterized via NMR-based metabolomics. The results of 13 shoot accessions revealed the identification and quantification of 23 primary and secondary metabolites belonging to various compound classes. Multivariate analyses were used for species classification, though found not successful in discrimination based on geographical origin. Nevertheless, C. citratus was found particularly enriched in neral, geranial, (E)-aconitic acid, isoorientin, and caffeic acid as the major characterizing metabolites compared to other species, while an unknown apigenin derivative appeared to discriminate C. martini. The high essential oil and phenolic content in C. citratus emphasizes its strong antioxidant activity, whereas (E)-aconitic acid accounts for its traditional use as insecticide. This study affords the first insight into metabolite compositional differences among Cymbopogon species. Moreover, antimicrobial, insecticidal, antidiabetic, and antioxidant compounds were identified that can be utilized as biomarkers for species authentication.