Dem IPB wird erneut ein beispielhaftes Handeln im Sinne einer chancengleichheitsorientierten Personal- und Organisationspolitik bescheinigt. Das Institut erhält zum 6. Mal in Folge das TOTAL E-QUALITY…
Die Plant Science Student Conference (PSSC) wird seit 20 Jahren im jährlichen Wechsel von Studierenden der beiden Leibniz-Institute IPK und IPB organisiert. Im Interview erläutern Christina Wäsch…
Fu, N.; Becker, T.; Brandt, W.; Kunert, M.; Burse, A.; Boland, W.;Involvement of
CYP347W1
in neurotoxin 3‐nitropropionic acid based chemical defense in mustard leaf beetle
Phaedon cochleariaeInsect Sci.29453–466(2022)DOI: 10.1111/1744-7917.12944
Chrysomelina beetles store 3-nitropropionic acid in form of a pre-toxin,
isoxazolin-5-one glucoside conjugated ester, to protect themselves
against predators. Here we identified a cytochrome P450 monooxygenase, CYP347W1, to be involved in the production of the 3-nitropropionic acid moiety of the isoxazolin-5-one glucoside ester. Knocking down CYP347W1
led to a significant depletion in the concentration of the
isoxazolin-5-one glucoside ester and an increase in the concentration of
the isoxazolin-5-one glucoside in the larval hemolymph. Enzyme assays
with the heterologously expressed CYP347W1 showed free β-alanine was not the direct substrate. Homology modeling indicated that β-alanine-CoA ester can fit into CYP347W1’s active site. Furthermore, we proved that Phaedon cochleariae eggs are not able to de novo
synthesize 3-NPA, although both isoxazolin-5-one glucoside and its
3-NPA conjugated ester are present in the eggs. These results provide
direct evidence for the involvement of CYP347W1 in the biosynthesis of a P. cochleariae chemical defense compound.