Dem IPB wird erneut ein beispielhaftes Handeln im Sinne einer chancengleichheitsorientierten Personal- und Organisationspolitik bescheinigt. Das Institut erhält zum 6. Mal in Folge das TOTAL E-QUALITY…
Die Plant Science Student Conference (PSSC) wird seit 20 Jahren im jährlichen Wechsel von Studierenden der beiden Leibniz-Institute IPK und IPB organisiert. Im Interview erläutern Christina Wäsch…
Parks, N. A.; Fischer, T. G.; Blankenburg, C.; Scalfani, V. F.; McEwen, L. R.; Herres-Pawlis, S.; Neumann, S.;The current landscape of author guidelines in chemistry through the lens of research data sharingPure and Applied Chemistryaop1-12(2023)DOI: 10.1515/pac-2022-1001
As the primary method of communicating research results, journals garner an enormous impact on community behavior. Publishing the underlying research data alongside journal articles is widely considered good scientific practice. Ideally, journals and their publishers place these recommendations or requirements in their author guidelines and data policies. Several efforts are working to improve the infrastructure, processes, and uptake of research data sharing, including the NFDI4Chem consortium, working groups within the RDA, and IUPAC, including the WorldFAIR Chemistry project. In this article, we present the results of a large-scale analysis of author guidelines from several publishers and journals active in chemistry research, showing how well the publishing
landscape supports different criteria and where there is room for improvement. While the requirement for deposition of X-ray diffraction data is commonplace, guidelines rarely mention machine-readable chemical structures and metadata/minimum information standards. Further evaluation criteria included recommendations on persistent identifiers, data availability statements, data deposition into repositories as well as of open analytical data formats. Our survey shows that publishers and journals are starting to include aspects of research data in their guidelines. We as authors should accept and embrace the guidelines with increasing requirements for data availability, data interoperability, and re-usability to improve chemistry research.
Preprints
Mik, V.; Poslíšil, T.; Brunoni, F.; Grúz, J.; Nožková, V.; Wasternack, C.; Miersch, O.; Strnad, M.; Floková, K.; Novák, O.; Široká, J.;Synthetic and analytical routes to the L-amino acid conjugates of cis-OPDA and their identification and quantification in plantsChemRxiv(2023)DOI: 10.26434/chemrxiv-2023-qlzj4
Cis-(+)-12-oxophytodienoic acid (cis-(+)-OPDA) is a bioactive jasmonate, a precursor of jasmonic acid, which also displays signaling activity on its own. Modulation of cis-(+)-OPDA actions may be carried out via biotransformation leading to metabolites of various functions, similar to other phytohormones. This work introduces a methodology for the synthesis of racemic cis-OPDA conjugates with amino acids (OPDA-aa) and their deuterium-labeled analogs, which enables the identification and accurate quantification of these compounds in plants. We have developed a highly sensitive liquid chromatography-tandem mass spectrometry-based method for the reliable determination of seven OPDA-aa (OPDA-Alanine, OPDA-Aspartate, OPDA-Glutamate, OPDA-Glycine, OPDA-Isoleucine, OPDA-Phenylalanine, and OPDA-Valine) from minute amount of plant material. The extraction from 10 mg of fresh plant tissue by 10% aqueous methanol followed by single-step sample clean-up on hydrophilic–lipophilic balanced columns prior to final analysis was optimized. The method was validated in terms of accuracy and precision, and the method parameters such as process efficiency, recovery and matrix effects were evaluated. In mechanically wounded 30-day-old Arabidopsis thaliana leaves, five endogenous (+)-OPDA-aa were identified and their endogenous levels reached a maximum of pmol/g. The time-course accumulation revealed a peak 60 min after the wounding, roughly corresponding to the accumulation of cis-(+)-OPDA. Current synthetic and analytical methodologies support studies on cis-(+)-OPDA conjugation with amino acids and research into the biological significance of these metabolites in plants.