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Bioinspired, synthetic porphyrin complexes are important catalysts in organic synthesis and play a pivotal role in efficient carbene transfer reactions. The advances in this research area stimulated recent, “chemo‐inspired” developments in biocatalysis. Today, both synthetic iron complexes and enzymes play an important role to conduct carbene transfer reactions. The advances and potential developments in both research areas are discussed in this concept article.
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Catalyst discovery and development requires the screening of large reaction sets necessitating analytic methods with the potential for high‐throughput screening. These techniques often suffer from substrate dependency or the requirement of expert knowledge. Chromatographic techniques (GC/LC) can overcome these limitations but are generally hampered by long analysis time or the need for special equipment. The herein developed multiple injections in a single experimental run (MISER) GC‐MS technique allows a substrate independent 96‐well microtiter plate analysis within 60 min. This method can be applied to any laboratory equipped with a standard GC‐MS. With this concept novel, unspecific peroxygenase (UPO) chimeras, could be identified, consisting of subdomains from three different fungal UPO genes. The GC‐technique was additionally applied to evaluate an YfeX library in an E. coli whole‐cell system for the carbene‐transfer reaction on indole, which revealed the thus far unknown axial heme ligand tryptophan.