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  1. IPB Halle
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    • Research Mission and Profile
    • Trenner 0
    • Molecular Signal Processing
      • Secretariat & All Staff
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        • Nutrient Sensing
        • Symbiosis Signaling
        • Jasmonate Signaling
    • Bioorganic Chemistry
      • Secretariat & All Staff
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        • Biofunctional Synthesis
        • Computational Chemistry
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    • Biochemistry of Plant Interactions
      • Secretariat & All Staff
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Eschen-Lippold, L.; Rosahl, S.; Westermann, B.; Arnold, N.; Aus Pilzen isolierte Substanz gegen den Erreger der Kraut- und Knollenfäule Kartoffelbau 63 18-21 (2012)
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0

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Eschen-Lippold, L.; Landgraf, R.; Smolka, U.; Schulze, S.; Heilmann, M.; Heilmann, I.; Hause, G.; Rosahl, S.; Activation of defense against Phytophthora infestans in potato by down-regulation of syntaxin gene expression New Phytol. 193 985-996 (2012) DOI: 10.1111/j.1469-8137.2011.04024.x
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• The oomycete Phytophthora infestans is the causal agent of late blight, the most devastating disease of potato. The importance of vesicle fusion processes and callose deposition for defense of potato against Phytophthora infestans was analyzed.• Transgenic plants were generated, which express RNA interference constructs targeted against plasma membrane‐localized SYNTAXIN‐RELATED 1 (StSYR1) and SOLUBLE N‐ETHYLMALEIMIDE‐SENSITIVE FACTOR ADAPTOR PROTEIN 33 (StSNAP33), the potato homologs of Arabidopsis AtSYP121 and AtSNAP33, respectively.• Phenotypically, transgenic plants grew normally, but showed spontaneous necrosis and chlorosis formation at later stages. In response to infection with Phytophthora infestans, increased resistance of StSYR1‐RNAi plants, but not StSNAP33‐RNAi plants, was observed. This increased resistance correlated with the constitutive accumulation of salicylic acid and PR1 transcripts. Aberrant callose deposition in Phytophthora infestans‐infected StSYR1‐RNAi plants coincided with decreased papilla formation at penetration sites. Resistance against the necrotrophic fungus Botrytis cinerea was not significantly altered. Infiltration experiments with bacterial solutions of Agrobacterium tumefaciens and Escherichia coli revealed a hypersensitive phenotype of both types of RNAi lines.• The enhanced defense status and the reduced growth of Phytophthora infestans on StSYR1‐RNAi plants suggest an involvement of syntaxins in secretory defense responses of potato and, in particular, in the formation of callose‐containing papillae.

Publications

Eschen-Lippold, L.; Rosahl, S.; Die Abwehr der Kartoffel gegen den Erreger der Kraut- und Knollenfäule Kartoffelbau 63 38-39 (2012)
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Eschen-Lippold, L.; Lübken, T.; Smolka, U.; Rosahl, S.; Characterization of potato plants with reduced StSYR1 expression Plant Signal Behav. 7 559-562 (2012) DOI: 10.4161/psb.19866
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Vesicle fusion processes in plants are important for both development and stress responses. Transgenic potato plants with reduced expression of SYNTAXIN-RELATED1 (StSYR1), a gene encoding the potato homolog of Arabidopsis PENETRATION1 (AtPEN1), display spontaneous necrosis and chlorosis at later stages of development. In accordance with this developmental defect, tuber number, weight and overall yield are significantly reduced in StSYR1-RNAi lines. Enhanced resistance of StSYR1-RNAi plants to Phytophthora infestans, the causal agent of late blight disease of potato, correlates with enhanced levels of salicylic acid, whereas levels of 12-oxophytodienoic acid and jasmonic acid are unaltered. Cultured cells of StSYR1-RNAi lines secrete at least two compounds which are not detectable in the supernatant of control cells, suggesting an involvement of StSYR1 in secretion processes to the apoplast.

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