The Analytical Laboratory is part of the Metabolomics Facility and primarily functions as a service unit for the entire IPB. It assists in isolating and chemically characterizing relevant metabolites to verify preliminary annotations and determine the structures of new metabolites, with a strong emphasis on NMR spectroscopy. Additionally, the structure elucidation is also supported by various optical spectroscopic methods (IR, UV, alpha, CD). Furthermore, this research unit is associated with a repository of reference compounds derived from plant and fungal metabolites and their derivatives collected in the institute.
The three NMR spectrometer available are used based on their equipment for different purposes. Basic 1D and 2D NMR experiments are necessary for the control of synthesis reactions and structure characterization of isolated natural products. If the concentration is sufficient (>3mg ) the samples were analyzed on the 400 and 500 MHz NMR machines. In cases of low concentrated samples, the 700 MHz NMR machine equipped with a cryo probe is used. The NMR-team can help with advanced experiments to answer the research questions that you have. It comprises experiments at different temperatures, signal-selective and bandselective NMR or the detection of other less abundant nuclei.
The characterization of unknown structures is a time consuming process and needs a lot of training. Therefore, the NMR-team can supports you in this task. Furthermore, a full elucidation support can be provided if necessary.
This page was last modified on 31 Jan 2025 01 Oct 2025 01 Oct 2025 .