The Agilent 1260 Bioinert HPLC system consists of a quaternary pump, a high capacity multisampler, and a thermostated column compartment. The flow path through these modules is metal-free, thus minimizing unwanted surface interactions of biological samples. The modules provide superior tolerance to high salt conditions and enable the use of solvents covering a wider pH range (between 2 and 13) compared to conventional HPLC systems.

The system is equipped with an Agilent 1290 DAD Detector (for UV and VIS detection) and an Agilent 1260 fluorescence detector.

Alternatively, analyte detection can proceed by amperometry using a pulsed amperometric detector (PAD) from Antec Scientific. This detector records the current if redox-active compounds are oxidized or reduced on an electrode. This detection method is commonly used for the analysis of carbohydrates after their separation on high performance anion exchange columns under high pH conditions.

Contact Person: Dr. Jörg Ziegler

Suitable for the analysis of:
Carbohydrates
UV / VIS active substances
Fluorescent molecules, e.g. Fmoc derivatized amines ( 10.1007/978-1-4939-9639-1_27)