Glandular Trichomes and Isoprenoid Biosynthesis

In this project, funded by the ERA-SynBio programme, our goal was to develop synthetic regulatory circuits to engineer orthogonal signal propagation in plants. One of the outputs was the development of the split-TALE system, where Transcription Activator-Like Effectors (TALES) are divided in two components, one binding a customized DNA sequence and the other activating transcription, which are brought together by an interacting pair of peptides fused to each of these components. We are now developing circuits based on the sequence specific RNAse Csy4 from the CRISPR for a tight and gene specific control of gene expression.

• Tom Schreiber (Postdoc)
• Anja Prange (Technician)