Comparative review on synthesis of cell wall polysaccharides in orthogonal hosts.
Plant cells are surrounded by an extracellular matrix that consists mainly of polysaccharides. Many molecular components involved in plant cell wall polymer synthesis have been identified, but it remains largely unknown how these molecular players function together to define the length and decoration pattern of a polysaccharide. By applying synthetic biology, entire biosynthetic machineries of the polysaccharide production were reconstructed successfully in yeast as an orthogonal host.
IPB scientist Dr. Cătălin Voiniciuc and researchers from Düsseldorf, Germany, recently published a review to evaluate the suitability of different hosts – Bacteria, Fungi and Animalia – for the synthesis of plant cell wall polysaccharides. The review compares methods, cost and time requirements as well as benefits and limitations of potential host organisms. Identification of suitable organisms could be useful for the production of polysaccharides and could significantly facilitate research in this field, in particular as single polysaccharides classes are challenging to study in only a single species.
