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This chapter presents an overview about the immunocytochemical techniques using in the model legume Medicago truncatula. After a short introduction into the basics of immunocytochemistry, detailed protocols that can be used to perform immunolabelling on light, confocal and electron microscopical level are listed. These protocols are successfully applied in the author’s laboratories to obtain information about the localization of various proteins in a range of tissues from Medicago truncatula. Depending on facilities to perform sectioning and the microscopical equipment, modifications might be required to suit individual demands. Therefore, please use these protocols as a starting point and adjust them after consulting experienced personnel at your local facilities.