logo ipb
logo ipb mobile
x
  • Deutsch
  • English
Benutzeranmeldung
  • Research
    • Research Mission and Profile

    • Molecular Signal Processing

      • Secretariat & All Staff
      • Technical Resources
      • Publications
      • Research Groups
        • Nutrient Sensing
          • Projects
          • Staff
          • Publications
          • Collaborations
        • Symbiosis Signaling
          • Projects
          • Staff
          • Publications
          • Collaborations
        • Jasmonate Signaling
          • Projects
          • Staff
          • Publications
          • Collaborations
    • Bioorganic Chemistry

      • Secretariat & All Staff
      • Technical Resources
      • Publications
      • Research Groups
        • Bioactives
          • Projects
          • Staff
          • Publications
          • Collaborations
        • Natural Products & Metabolomics
          • Projects
          • Staff
          • Publications
          • Collaborations
        • Biotechnology
          • Projects
          • Staff
          • Publications
          • Collaborations
        • Biofunctional Synthesis
          • Projects
          • Staff
          • Publications
          • Collaborations
        • Computational Chemistry
          • Projects
          • Staff
          • Publications
          • Collaborations
        • Data & Resources
          • Projects
          • Staff
          • Publications
          • Collaborations
    • Biochemistry of Plant Interactions

      • Secretariat & All Staff
      • Technical Resources
      • Publications
      • Research Groups
        • Calcium-dependent Protein Kinases
          • Projects
          • Staff
          • Publications
          • Collaborations
        • Cellular Signaling
          • Projects
          • Staff
          • Publications
          • Collaborations
        • Metabolite-based Defense Mechanisms
          • Projects
          • Staff
          • Publications
          • Collaborations
        • Nuclear Processes in Plant Defense
          • Projects
          • Staff
          • Publications
          • Collaborations
    • Cell and Metabolic Biology

      • Secretariat & All Staff
      • Technical Resources
      • Publications
      • Research Groups
        • Glandular Trichomes and Isoprenoid Biosynthesis
          • Projects
          • Staff
          • Publications
          • Collaborations
        • Jasmonate Function & Mycorrhiza
          • Projects
          • Staff
          • Publications
          • Collaborations
        • Phenylpropanoid Metabolism
          • Projects
          • Staff
          • Publications
          • Collaborations
        • Synthetic Biology
          • Projects
          • Staff
          • Publications
          • Collaborations
    • Independent Junior Research Groups

      • Research Groups
        • Receptor Biochemistry
          • Projects
          • Staff
          • Publications
          • Collaborations
    • Program Center MetaCom

      • Secretariat & All staff
      • Publikationen
      • Our Equipment
      • Research Groups
        • Metabolomics Facility
          • Projects
          • Staff
          • Publications
          • Methods
        • MetaCom Analytical Laboratory
          • Projects
          • Staff
          • Publications
          • Methods
        • Computational Plant Biochemistry
          • Projects
          • Staff
          • Publications
          • Collaborations
        • MetaCom Junior Research Group
          • Projects
          • Staff
    • Publications

    • Good Scientific Practice

    • Research Funding

    • Networks and Collaborative Projects

      • Collaborative Projects as Coordinator
        • Completed Projects as Coordinator
      • Collaborative Projects as Partner
        • Completed Projects as Partner
      • Networks
    • Symposia and Colloquia

      • Lectures
        • IPB Seminars
      • Leibniz Plant Biochemistry Symposia
        • 2025 Symposium
    • Alumni Research Groups

      • Research Groups
        • Auxin Signaling
          • Projects
          • Publications
        • Bioorganic Chemistry
          • Projects
          • Publications
        • Designer Glycans
          • Projects
          • Publications
        • Jasmonate Mode of Action
          • Publications
        • Protein Recognition and Degradation
          • Projects
          • Publications
        • Regulatory RNAs (MLU-associated group)
          • Projects
          • Publications
        • Signal Integration
          • Projects
          • Publications
        • Ubiquitination in Immunity
          • Projects
          • Publications
        • Cellular Coordination
          • Projects
          • Publications
  • Infrastructure
    • Databases and Tools

      • XCMS
      • Rdisop
      • CAMERA
      • MetShot
      • MassBank
      • MetFrag
      • MetFamily
      • PaCeQuant
      • CytoskeletonAnalyzer
      • GoldenMutagenesis
      • cisHighlight
      • FlagScreen
      • RootDetection
    • Technical Resources

    • Imaging Unit

    • Greenhouses and Phytochambers

    • Library Services

      • OPAC
      • Electronic Journals Library
      • Service for Employees
  • Institute
    • Organizational Chart

    • Management and Boards

      • Board of Trustees
      • Scientific Advisory Board
      • IPB Management / Board of Directors
      • Scientific Council
      • Authorized Representatives of the IPB
      • Staff Council
      • Statutes
    • Administration and Infrastructure

      • Secretariat & All Staff
      • Working Groups
        • Human Resources
        • Finance & Accounting
        • Purchasing
        • IT & Technical Support
        • Experimental Nursery
        • Facility Management
        • Library
        • Digitalization
    • Energy Management

      • Objectives and Measures
      • Energy Management Team
    • Diversity, Family, Equality

      • Diversity
      • Equality
      • Family Support
      • Training and further Education
      • Integration and Health
      • General Equal Treatment Act
    • Public Tendering

    • Patents and Licensing

    • The IPB Welcoming Culture

    • Guest Houses

    • IPB Site Map

    • Brief IPB History

      • Bildergalerie zur Historie
      • Alte Filmsequenzen zum Institut
      • Historischer Massenspektrograph
    • Alumni Network

      • The IPB as a career launching pad
  • Career
    • Data protection information for applicants

    • PhD Program

      • PhD Student Representatives
      • DoCou - Doctoral Training Courses
      • PSSC
    • Postdocs

    • Berufsausbildung

  • Public Relations
    • News

      • 2024
      • 2023
      • 2022
      • Archiv Aktuelles
        • 2021
        • 2020
        • 2019
        • 2018
        • 2017
        • 2016
        • 2015
        • 2014
        • vor 2014
    • News Ticker Science

      • News Ticker 2024
      • News Ticker 2023
      • News Ticker 2022
      • News Ticker Archive
        • News Ticker 2021
        • News Ticker 2020
        • News Ticker 2019
    • Press Releases

      • 2024
      • 2023
      • 2022
      • Archive Press Releases
        • 2021
        • 2020
        • 2019
        • 2018
        • 2017
        • 2016
        • 2015
        • 2014
        • 2013
        • 2012
        • 2011
        • 2010
        • 2009
        • 2008
        • 2007
        • 2006
        • 2005
        • 2004
        • 2003
        • 2002
    • IPB Pressespiegel

    • LANGE NACHT, DIE WISSEN SCHAFFT: PROGRAMM

    • IPB Newsletter

    • Printed / Information Material

    • Scientific Reports / Research Highlights

    • Events

      • 2024 Long Night of Sciences
      • 2024 Leibniz Plant Biochemistry Symposium
      • Archiv Veranstaltungen
    • Cover Art

    • Citizen Science: Pilzberatung

      • Das Reich der Pilze
      • Pilzberatung
      • Forschung an Pilzen
  • Contact
    • Directions for Visitors

    • Staff Directory

    • Imprint

    • Data Protection

    • Accessibility

  1. IPB Halle
  2. Research
  3. Publications

    • Research Mission and Profile
    • Trenner 0
    • Molecular Signal Processing
      • Secretariat & All Staff
      • Technical Resources
      • Publications
      • Research Groups
        • Nutrient Sensing
        • Symbiosis Signaling
        • Jasmonate Signaling
    • Bioorganic Chemistry
      • Secretariat & All Staff
      • Technical Resources
      • Publications
      • Research Groups
        • Bioactives
        • Natural Products & Metabolomics
        • Biotechnology
        • Biofunctional Synthesis
        • Computational Chemistry
        • Data & Resources
    • Biochemistry of Plant Interactions
      • Secretariat & All Staff
      • Technical Resources
      • Publications
      • Research Groups
        • Calcium-dependent Protein Kinases
        • Cellular Signaling
        • Metabolite-based Defense Mechanisms
        • Nuclear Processes in Plant Defense
    • Cell and Metabolic Biology
      • Secretariat & All Staff
      • Technical Resources
      • Publications
      • Research Groups
        • Glandular Trichomes and Isoprenoid Biosynthesis
        • Jasmonate Function & Mycorrhiza
        • Phenylpropanoid Metabolism
        • Synthetic Biology
    • Independent Junior Research Groups
      • Research Groups
        • Receptor Biochemistry
    • Program Center MetaCom
      • Secretariat & All staff
      • Publikationen
      • Our Equipment
      • Research Groups
        • Metabolomics Facility
        • MetaCom Analytical Laboratory
        • Computational Plant Biochemistry
        • MetaCom Junior Research Group
    • Trenner 1
    • Publications
    • Good Scientific Practice
    • Research Funding
    • Trenner
    • Networks and Collaborative Projects
      • Collaborative Projects as Coordinator
        • Completed Projects as Coordinator
      • Collaborative Projects as Partner
        • Completed Projects as Partner
      • Networks
    • Symposia and Colloquia
      • Lectures
        • IPB Seminars
      • Leibniz Plant Biochemistry Symposia
        • 2025 Symposium
    • Trenner
    • Alumni Research Groups
      • Research Groups
        • Auxin Signaling
        • Bioorganic Chemistry
        • Designer Glycans
        • Jasmonate Mode of Action
        • Protein Recognition and Degradation
        • Regulatory RNAs (MLU-associated group)
        • Signal Integration
        • Ubiquitination in Immunity
        • Cellular Coordination

Advanced Search

  • Type of publication
    • Publication (2)
  • Year
    • 2020 (1)
      2024 (1)
  • Journal / Volume / Preprint Server Sorted by frequency and by alphabetical order
    • Plant Cell (2)
  • Author Sorted by frequency and by alphabetical order
    • Hause, B. (8)
      Lee, J. (7)
      Scheel, D. (6)
      Wasternack, C. (6)
      Hause, G. (5)
      Hoehenwarter, W. (5)
      Schuster, M. (5)
      Böttcher, C. (4)
      Eschen-Lippold, L. (4)
      Marillonnet, S. (4)
      Romeis, T. (4)
      Dissmeyer, N. (3)
      Heilmann, I. (3)
      Matschi, S. (3)
      Nowack, M. K. (3)
      Nürnberger, T. (3)
      Rosahl, S. (3)
      Schnittger, A. (3)
      Stenzel, I. (3)
      Trujillo, M. (3)
      Athmer, B. (2)
      Dohmann, E. M. (2)
      Furlan, G. (2)
      Glawischnig, E. (2)
      Hake, K. (2)
      Herde, M. (2)
      Ischebeck, T. (2)
      Jiang, X. (2)
      Kramell, R. (2)
      Krishnamoorthy, P. (2)
      Lederer, S. (2)
      Lee, C.-W. (2)
      Majovsky, P. (2)
      Miersch, O. (2)
      Parker, J. E. (2)
      Pusch, S. (2)
      Schulze, W. X. (2)
      Schwechheimer, C. (2)
      Tissier, A. (2)
      Turner, J. G. (2)
      Weimer, A. K. (2)
      Westphal, L. (2)
      Wirthmueller, L. (2)
      Wirtz, M. (2)
      Witte, C.-P. (2)
      Acosta, I. F. (1)
      Alsharafa, K. (1)
      Altmann, S. (1)
      Anderson, R. G. (1)
      Antolín-Llovera, M. (1)
      Atzorn, R. (1)
      Baginsky, S. (1)
      Balazadeh, S. (1)
      Balcerowicz, D. (1)
      Balcke, G. U. (1)
      Bao, Z. (1)
      Barry, C. S. (1)
      Bennewitz, S. (1)
      Bergau, N. (1)
      Betsuyaku, S. (1)
      Bevan, M. (1)
      Bhat, R. (1)
      Bi, D. (1)
      Binarová, P. (1)
      Blum, E. (1)
      Blume, B. (1)
      Blée, E. (1)
      Bolger, A. M. (1)
      Boss, W. F. (1)
      Bouyer, D. (1)
      Brandt, W. (1)
      Brash, A. (1)
      Bravo-Cazar, A. L. (1)
      Brearley, C. (1)
      Brillada, C. (1)
      Bruffett, K. (1)
      Brunner, F. (1)
      Brüx, A. (1)
      Bucher, M. (1)
      Busch, W. (1)
      Bögre, L. (1)
      Bürstenbinder, K. (1)
      Cagna, G. (1)
      Calderon-Villalobos, L. I. (1)
      Calderon-Villalobos, L. I. A. (1)
      Cenklová, V. (1)
      Chapple, C. (1)
      Cheng, Y. T. (1)
      Colby, T. (1)
      Costa, A. (1)
      Courty, P.-E. (1)
      Culley, L. K. (1)
      Cusumano, J. C. (1)
      Darwish, E. (1)
      De Masi, R. (1)
      De Schutter, K. (1)
      De Veylder, L. (1)
      De Winter, F. (1)
      Dean, G. H. (1)
      Deeken, R. (1)
  • Year
  • Type of publication
Search narrowed by: Journal / Volume / Preprint Server Sorted by frequency and by alphabetical order: Plant Cell Author Sorted by frequency and by alphabetical order: Schulze, W. X. Remove all filters
Displaying results 1 to 2 of 2.
  • Results as:
  • Print view
  • Endnote (RIS)
  • BibTeX
  • Table: CSV | HTML
Results per page:
  • 1

Publications

Liese, A.; Eichstädt, B.; Lederer, S.; Schulz, P.; Oehlschläger, J.; Matschi, S.; Feijó, J. A.; Schulze, W. X.; Konrad, K. R.; Romeis, T.; Imaging of plant calcium-sensor kinase conformation monitors real time calcium-dependent decoding in planta Plant Cell 36 276-296 (2024) DOI: 10.1093/plcell/koad196
  • Abstract
  • Internet
  • BibText
  • RIS

Changes in cytosolic calcium (Ca2+) concentration are among the earliest reactions to a multitude of stress cues. While a plethora of Ca2+-permeable channels may generate distinct Ca2+ signatures and contribute to response specificities, the mechanisms by which Ca2+ signatures are decoded are poorly understood. Here we developed a genetically encoded FRET (Förster resonance energy transfer)-based reporter that visualizes the conformational changes in Ca2+-dependent protein kinases (CDPKs/CPKs). We focused on two CDPKs with distinct Ca2+-sensitivities, highly Ca2+-sensitive Arabidopsis (Arabidopsis thaliana) AtCPK21 and rather Ca2+-insensitive AtCPK23, to report conformational changes accompanying kinase activation. In tobacco (Nicotiana tabacum) pollen tubes, which naturally display coordinated spatial and temporal Ca2+ fluctuations, CPK21-FRET, but not CPK23-FRET, reported oscillatory emission ratio changes mirroring cytosolic Ca2+ changes, pointing to the isoform-specific Ca2+-sensitivity and reversibility of the conformational change. In Arabidopsis guard cells, CPK21-FRET-monitored conformational dynamics suggest that CPK21 serves as a decoder of signal-specific Ca2+ signatures in response to abscisic acid and the flagellin peptide flg22. Based on these data, CDPK-FRET is a powerful approach for tackling real-time live-cell Ca2+ decoding in a multitude of plant developmental and stress responses.

Publications

Durian, G.; Sedaghatmehr, M.; Matallana-Ramirez, L. P.; Schilling, S. M.; Schaepe, S.; Guerra, T.; Herde, M.; Witte, C.-P.; Mueller-Roeber, B.; Schulze, W. X.; Balazadeh, S.; Romeis, T.; Calcium-Dependent Protein Kinase CPK1 Controls Cell Death by In Vivo Phosphorylation of Senescence Master Regulator ORE1 Plant Cell 32 1610-1625 (2020) DOI: 10.1105/tpc.19.00810
  • Abstract
  • BibText
  • RIS

Calcium-regulated protein kinases are key components of are key components of intracellular signaling in plants that mediate rapid stress-induced responses to changes in the environment. To identify in vivo phosphorylation substrates of CALCIUM-DEPENDENT PROTEIN KINASE1 (CPK1), we analyzed the conditional expression of constitutively active CPK1 in conjunction with in vivo phosphoproteomics. We identified Arabidopsis thaliana ORESARA1 (ORE1), the developmental master regulator of senescence, as a direct CPK1 phosphorylation substrate. CPK1 phosphorylates ORE1 at a hotspot within an intrinsically disordered region. This augments transcriptional activation by ORE1 of its downstream target gene BIFUNCTIONAL NUCLEASE1 (BFN1). Plants that overexpress ORE1, but not an ORE1 variant lacking the CPK1 phosphorylation hotspot, promote early senescence. Furthermore, ORE1 is required for enhanced cell death induced by CPK1 signaling. Our data validate the use of conditional expression of an active enzyme combined with phosphoproteomics to decipher specific kinase target proteins of low abundance, of transient phosphorylation, or in yet undescribed biological contexts. Here, we have identified that senescence is not just under molecular surveillance manifested by stringent gene regulatory control over ORE1. In addition, the decision to die is superimposed by an additional layer of control towards ORE1 via its post-translational modification linked to the calcium-regulatory network through CPK1.

  • 1

Print

  • IPB Halle
  • News
  • Lectures
  • Publications
  • Public Tendering
  • IPB Remote & Mail
  • Imprint
  • Data Protection
  • Accessibility
  • Die Leibniz-Gemeinschaft
  • Wege zu einer pflanzenbasierten Wirtschaft
  • Martin-Luther Universität Halle
  • Erfolgsfaktor Familie
  • TOTAL E-QUALITY
  • Research
    • Research Mission and Profile

    • Molecular Signal Processing

    • Bioorganic Chemistry

    • Biochemistry of Plant Interactions

    • Cell and Metabolic Biology

    • Independent Junior Research Groups

    • Program Center MetaCom

    • Publications

    • Good Scientific Practice

    • Research Funding

    • Networks and Collaborative Projects

    • Symposia and Colloquia

    • Alumni Research Groups

  • Infrastructure
    • Databases and Tools

    • Technical Resources

    • Imaging Unit

    • Greenhouses and Phytochambers

    • Library Services

  • Institute
    • Organizational Chart

    • Management and Boards

    • Administration and Infrastructure

    • Energy Management

    • Diversity, Family, Equality

    • Public Tendering

    • Patents and Licensing

    • The IPB Welcoming Culture

    • Guest Houses

    • IPB Site Map

    • Brief IPB History

    • Alumni Network

  • Career
    • Data protection information for applicants

    • PhD Program

    • Postdocs

    • Berufsausbildung

  • Public Relations
    • News

    • News Ticker Science

    • Press Releases

    • IPB Pressespiegel

    • LANGE NACHT, DIE WISSEN SCHAFFT: PROGRAMM

    • IPB Newsletter

    • Printed / Information Material

    • Scientific Reports / Research Highlights

    • Events

    • Cover Art

    • Citizen Science: Pilzberatung

  • IPB Remote & Mail