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Publications

Püllmann, P.; Weissenborn, M. J.; Pilzliche Peroxygenasen: der Schlüssel zu C-H-Hydroxylierungen und mehr? BIOspektrum 25 572-574 (2019) DOI: 10.1007/s12268-019-1090-2
  • Abstract
  • Internet
  • BibText
  • RIS

Fungal peroxygenases represent an exciting new enzyme class for stereo-selective hydroxylation reactions. They are capable of the oxyfunctionalisation of a large, diverse scope of substrates including alkanes and steroids as well as the heteroatoms sulfur and nitrogen. The outstanding activities and stabilities as well as their reliance on hydrogen peroxide as co-substrate renders it a highly interesting biocatalyst.

Publications

Reisberg, M.; Arnold, N.; Porzel, A.; Neubert, R. H.; Dräger, B.; Malusides, novel glucosylceramides isolated from apple pomace (Malus domestica) Z. Naturforsch. C 73 33-39 (2018) DOI: 10.1515/znc-2017-0059
  • Abstract
  • BibText
  • RIS

Three new glucosylceramides (GluCers) named malusides I–III (1–3) were isolated from apple (cultivars of Malus domestica) pomace (fruit material remaining after juice extraction). An unusual oxo/hydroxy group pattern within the sphingadienine (d18:2) type sphingoid base was observed. All compounds contained the same α-hydroxylated fatty acid (h16:0) and a β-D-glucose moiety. Their structures were assigned on the basis of one- and two-dimensional (1D and 2D) nuclear magnetic resonance (NMR) spectroscopic analyses and mass spectrometry (MS) measurements.

Publications

Kammel, M.; Knorrscheidt, A.; Püllmann, P.; Weissenborn, M. J.; Tackling the numbers problem: Entwicklung nicht-nativer Enzymreaktionen BIOspektrum 23 830-832 (2017) DOI: 10.1007/s12268-017-0876-3
  • Abstract
  • BibText
  • RIS

The screening effort of large protein variant libraries renders the probability of coincidental discovering a new enzyme with non-natural activity to almost zero - the so-called numbers problem. Insights into the origin of life, evolution and enzymatic promiscuity, combined with the inspiration of methods from organic chemistry, offer solutions for this problem. With the newly discovered enzymes synthetic micro production units shall be established in a Leibniz Research Cluster where engineering and biotechnology are combined.

Publications

Berger, R.; Bornscheuer, U.; Liese, A.; Schwaneberg, U.; Syldatk, C.; Wessjohann, L.; Biotechnologie von Morgen: DECHEMA/VAAM-Fachgruppe „Biotransformationen“ BIOspektrum 19 208-210 (2013) DOI: 10.1007/s12268-013-0291-3
  • BibText
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0

Publications

Wessjohann, L.; Vogt, T.; Kufka, J.; Klein, R.; Prenyl- und Methyltransferasen in Natur und Synthese BIOspektrum 18 22-25 (2012) DOI: 10.1007/s12268-012-0137-4
  • Abstract
  • BibText
  • RIS

Late stage enzymatic prenylation and methylation are means to diversify (natural) compounds and to specify their functions. In eukaryotes and microbes, these steps are performed by large enzyme families, the prenyl and methyl transferases, which modify various types of small molecules, like isoprenoids, phenolics or alkaloids, but also DNA and proteins. We investigate the theoretical basis of these processes and possible commercial applications in synthetic chemistry.

Publications

Dissmeyer, N.; CDK-Phosphorylierung in Zellzyklus und Stress: artspezifische Unterschiede BIOspektrum 751-753 (2010)
  • Abstract
  • BibText
  • RIS

In Arabidopsis, deactivation of cyclin-dependent kinases via phosphorylation has no function in cell proliferation, growth, and stress response. In other eukaryotes, however, this is mandatorily required for maintaining genomic integrity.

Publications

Lusebrink, I.; Dettner, K.; Schierling, A.; Müller, T.; Daolio, C.; Schneider, B.; Schmidt, J.; Seifert, K.; New Pyridine Alkaloids from Rove Beetles of the Genus Stenus (Coleoptera: Staphylinidae) Z. Naturforsch. C 64 271-278 (2009) DOI: 10.1515/znc-2009-3-420
  • Abstract
  • BibText
  • RIS

Three new pyridine alkaloids were detected in the pygidial glands of some Stenus species. The chemotaxonomic significance of the occurrence of these alkaloids and stenusine in different Stenus species is discussed. The antimicrobial properties of (Z)- and (E)-3-(2- methyl-1-butenyl)-pyridine and the deterrent activities of stenusine and norstenusine were investigated.

Publications

Stintzing, F.; Schliemann, W.; Pigments of Fly Agaric (Amanita muscaria) Z. Naturforsch. C 62 779-785 (2007) DOI: 10.1515/znc-2007-11-1201
  • Abstract
  • BibText
  • RIS

The complex pigment pattern of fly agaric (Amanita muscaria) cap skins has been studied by LC-DAD and mass spectrometry. Among the betaxanthins the corresponding derivatives of serine, threonine, ethanolamine, alanine, Dopa, phenylalanine and tryptophan are reported for the first time to contribute to the pigment pattern of fly agarics. Betalamic acid, the chromophoric precursor of betaxanthins and betacyanins, muscaflavin and seco-dopas were also detected. Furthermore, the red-purple muscapurpurin and the red muscarubrin were tentatively assigned while further six betacyanin-like components could not be structurally allocated. Stability studies indicated a high susceptibility of pigment extracts to degradation which led to rapid colour loss thus rendering a complete characterization of betacyaninlike compounds impossible at present. Taking into account these difficulties the presented results may be a starting point for a comprehensive characterization of the pigment composition of fly agarics.

Publications

Aulinger, K.; Arnold, N.; Steglich, W.; Metabolites of 2-Aminophenol from Fruit Bodies of Lepiota americana (Agaricales) Z. Naturforsch. C 55 481-484 (2000) DOI: 10.1515/znc-2000-5-628
  • Abstract
  • BibText
  • RIS

From the acetone extract of the North American toadstool Lepiota americana 2-aminophenoxazin-3-one (1) and a novel amino-1,4-benzoquinone derivative, lepiotaquinone (2), were isolated. The structure of 2 was confirmed by its preparation from 2-aminophenol and amino-1,4-benzoquinone.

Publications

Schmidt, J.; Richter, K.; Voigt, B.; Adam, G.; Metabolic Transformation of the Brassinosteroid 24-Epi-castasterone by the Cockroach Periplaneta americana Z. Naturforsch. C 55 233-239 (2000) DOI: 10.1515/znc-2000-3-415
  • Abstract
  • BibText
  • RIS

After feeding of 24-epi-castasterone to the cockroach Periplaneta americana an organspecific epimerization of the brassinosteroid to 2,24-depi-castasterone could be detected in female insects. The metabolite being observed only in the ovaries and not in the testes of the insect was identified by GC/MS in comparison with a synthesized authentic sample. Contrary, 24-epi-brassinolide is not metabolized in the sexual organs of Periplaneta americana. This is the first evidence of a metabolic transformation of a brassinosteroid in insects.

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