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Books and chapters

Stenzel, I.; Hause, B.; Feussner, I.; Wasternack, C.; Transcriptional Activation of Jasmonate Biosynthesis Enzymes is not Reflected at Protein Level 267-270 (2003) DOI: 10.1007/978-94-017-0159-4_62
  • Abstract
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Jasmonic acid (JA) and its precursor 12-oxo phytodienoic acid (OPDA) are lipid-derived signals in plant stress responses and development (Wasternack and Hause, 2002). Within the wound-response pathway of tomato, a local response of expression of defense genes such as the proteinase inhibitor 2 gene (PIN2) is preceded by a rise in JA (Herde et al., 1996; Howe et al., 1996) and ethylene (O’Donnell et al., 1996). Mutants affected in JA biosynthesis such as defl (Howe et al., 1996) or spr-2 (Li et al., 2002) clearly indicated that JA biosynthesis is an ultimate part of wound signaling. It is less understood, however, how the rise in JA is regulated.

Books and chapters

Lee, J.; Nürnberger, T.; Is Pore Formation Activity of HrpZ Required for Defence Activation in Plant Cells? 165-173 (2003) DOI: 10.1007/978-94-017-0133-4_18
  • Abstract
  • BibText
  • RIS

The HrpZ gene product, harpin, is an export substrate of the type III secretion system of phytopathogenic Pseudomonas syringae. The role of this protein in pathogenesis is largely unknown. We previously determined that HrpZ binds to lipids and can form cation pores in synthetic lipid bilayers. Such pore-forming activity may allow nutrient release during bacterial colonisation of host plants. In addition. HrpZ is known to trigger plant defence responses in a variety of plants, such as tobacco. We have previously also characterised a binding site in tobacco plasma membranes that likely mediates HrpZ-induced defence responses. In order to reconcile these findings, we pose the question as to whether the activation of plant defence responses by HrpZ is mediated through a “classical” receptor perception mode or if plant membrane perturbation through the inherent pore-forming activity of HrpZ may induce defence responses. As defence in parsley cells can be induced both in a receptor-mediated manner or through ionophores these cells served as an ideal system for our analysis. We first performed ligand binding studies to characterise the presence of a binding site/receptor. We further digested HrpZ with endopeptidases and used subfragments of HrpZ to assess the elicitor-active domain of HrpZ. A C-terminal region of HrpZ appears to be sufficient to elicit plant defence responses. A novel assay involving dye-loaded liposomes was developed to validate previous electrophysiological findings on HrpZ-mediated cation pore formation. More importantly, this assay was used to establish if the elicitor-active C-terminal fragment of HrpZ could form pores. Our findings suggest that the structural requirements for ion pore formation and activation of plant defence responses by HrpZ are different. Thus, ion pore formation alone may not explain the activation of plant defence by HrpZ.

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Scheel, D.; Oxidative burst and the role of reactive oxygen species in plant-pathogen interactions Inzé, D. & van Montagu, M., eds. 137-153 (2002)
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0

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Faust, J.; Wrenger, S.; Reinhold, D.; Kähne, T.; Lorey, S.; Stöckel-Maschek, A.; Brandt, W.; Mrestani-Klaus, C.; Stiebitz, B.; Fuchs, P.; Ansorge, S.; Neubert, K.; Down regulation of T-cell activation by synthetic dipeptidyl peptidase IV inhibitors with the N.terminal MXP sequence Bendetti, E. & Predone, C., eds. 750-751 (2002)
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0

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Clemens, S.; Thomine, S.; Schroeder, J. I.; Molecular mechanisms that control plant tolerance to heavy metals and possible roles towards manipulating metal accumulation 665-691 (2002)
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0

Books and chapters

Bruns, I.; Sutter, K.; Neumann, D.; Krauss, G.-J.; Glutathione accumulation - a specific response of mosses to heavy metal stress 389-391 (2000)
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0

Books and chapters

Scheel, D.; Blume, B.; Brunner, F.; Fellbrich, G.; Dalbøge, H.; Hirt, H.; Kauppinen, S.; Kroj, T.; Ligterink, W.; Nürnberger, T.; Tschöpe, M.; Zinecker, H.; zur Nieden, U.; Receptor-mediated signal transduction in plant defense 131-135 (2000)
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0

Books and chapters

Wessjohann, L. A.; Sontag, B.; Dessoy, M. A.; Enzymatic C-C-Coupling: The Development of Aromatic Prenylation for Organic Synthesis 79-88 (1999)
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0

Books and chapters

Feussner, I.; Balkenhohl, T. J.; Porzel, A.; Kühn, H.; Wasternack, C.; Structural Elucidation of Oxygenated Triacylglycerols in Cucumber and Sunflower Cotyledons 57-58 (1998)
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0

Books and chapters

Feussner, I.; Blée, E.; Weichert, H.; Rousset, C.; Wasternack, C.; Fatty Acid Catabolism at the Lipid Body Membrane of Germinating Cucumber Cotyledons 311-313 (1998)
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