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  1. IPB Halle
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    • Research Mission and Profile
    • Trenner 0
    • Molecular Signal Processing
      • Secretariat & All Staff
      • Technical Resources
      • Publications
      • Research Groups
        • Nutrient Sensing
        • Symbiosis Signaling
        • Jasmonate Signaling
    • Bioorganic Chemistry
      • Secretariat & All Staff
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        • Bioactives
        • Natural Products & Metabolomics
        • Biotechnology
        • Biofunctional Synthesis
        • Computational Chemistry
        • Data & Resources
    • Biochemistry of Plant Interactions
      • Secretariat & All Staff
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      • Publications
      • Research Groups
        • Calcium-dependent Protein Kinases
        • Cellular Signaling
        • Metabolite-based Defense Mechanisms
        • Nuclear Processes in Plant Defense
    • Cell and Metabolic Biology
      • Secretariat & All Staff
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    • Publications
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    • Alumni Research Groups
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        • Auxin Signaling
        • Bioorganic Chemistry
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        • Jasmonate Mode of Action
        • Protein Recognition and Degradation
        • Regulatory RNAs (MLU-associated group)
        • Signal Integration
        • Ubiquitination in Immunity
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Hornung, E.; Krueger, C.; Pernstich, C.; Gipmans, M.; Porzel, A.; Feussner, I.; Production of (10E,12Z)-conjugated linoleic acid in yeast and tobacco seeds BBA-Mol. Cell Biol. Lipids 1738 105-114 (2005) DOI: 10.1016/j.bbalip.2005.11.004
  • Abstract
  • BibText
  • RIS

The polyenoic fatty acid isomerase from Propioniumbacterium acnes (PAI) was expressed in E. coli and biochemically characterized. PAI catalyzes the isomerization of a methylene-interrupted double bond system to a conjugated double bond system, creating (10E,12Z)-conjugated linoleic acid (CLA). PAI accepted a wide range of free polyunsaturated fatty acids as substrates ranging from 18:2 fatty acids to 22:6, converting them to fatty acids with two or three conjugated double bonds. For expression of PAI in yeast the PAI-sequence encoding 20 N-terminal amino acid residues was altered for optimal codon usage, yielding codon optimized PAI (coPAI). The percentage of 10,12-CLA of total esterified fatty acids was 8 times higher in yeast transformed with coPAI than in cells transformed with PAI. CLA was detected in amounts up to 5.7% of total free fatty acids in yeast transformed with coPAI but none was detected in yeast transformed with PAI. PAI or coPAI under the control of the constitutive CaMV 35S promoter or the seed-specific USP promoter was transformed into tobacco plants. CLA was only detected in seeds in coPAI-transgenic plants. The amount of CLA detected in esterified fatty acids was up to 0.3%, in free fatty acids up to 15%.

Publications

Thuy, T. T.; Porzel, A.; Franke, K.; Wessjohann, L.; Sung, T. V.; Isoquinolone and protoberberine alkaloids from Stephania rotunda Pharmazie 60 701-704 (2005)
  • Abstract
  • BibText
  • RIS

Chemical investigation of Stephania rotunda Lour. growing in Viet Nam led to the isolation and structural elucidation of three new alkaloids, 5-hydroxy-6,7-dimethoxy-3,4-dihydroisoquinolin-1(2H)-one (1), thaicanine 4-O-β-L-glucoside (6), as well as (–)-thaicanine N-oxide (4-hydroxycorynoxidine) (8), along with 23 known alkaloids. These structures were determined on the basis of MS and NMR spectroscopic data.

Publications

Teichert, A.; Lübken, T.; Schmidt, J.; Porzel, A.; Arnold, N.; Wessjohann, L.; Unusual Bioactive 4-Oxo-2-alkenoic Fatty Acids from Hygrophorus eburneus Z. Naturforsch. B 60 25-32 (2005) DOI: 10.1515/znb-2005-0105
  • Abstract
  • BibText
  • RIS

From fruit bodies of the basidiomycete Hygrophorus eburneus (Bull.: Fr.) Fr. (Tricholomataceae) eight fatty acids (C16, C18) with γ -oxocrotonate partial structure could be isolated. Initial tests demonstrate their bactericidal and fungicidal activity. The structures of (2E,9E)-4-oxooctadeca- 2,9,17-trienoic acid (1), (2E,11Z)-4-oxooctadeca-2,11,17-trienoic acid (2), (E)-4-oxohexadeca-2,15- dienoic acid (3), (E)-4-oxooctadeca-2,17-dienoic acid (4), (2E,9E)-4-oxooctadeca-2,9-dienoic acid (5), (2E,11Z)-4-oxooctadeca-2,11-dienoic acid (6), (E)-4-oxohexadec-2-enoic acid (7), and (E)-4- oxooctadec-2-enoic acid (8) were elucidated on the basis of their spectroscopic data.

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