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Molecular Signal Processing
Bioorganic Chemistry
Biochemistry of Plant Interactions
Cell and Metabolic Biology
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Publications - Cell and Metabolic Biology
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The discovery of proteins with programmable DNA-binding specificities triggered a whole array of applications in synthetic biology, including genome editing, regulation of transcription, and epigenetic modifications. Among those, transcription activator-like effectors (TALEs) due to their natural function as transcription regulators, are especially well-suited for the development of orthogonal systems for the control of gene expression. We describe here the construction and testing of libraries of synthetic TALE-activated promoters which are under the control of a single TALE with a given DNA-binding specificity. These libraries consist of a fixed DNA-binding element for the TALE, a TATA box, and variable sequences of 19 bases upstream and 43 bases downstream of the DNA-binding element. These libraries were cloned using a Golden Gate cloning strategy making them usable as standard parts in a modular cloning system. The broad range of promoter activities detected and the versatility of these promoter libraries make them valuable tools for applications in the fine-tuning of expression in metabolic engineering projects or in the design and implementation of regulatory circuits.
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Bisher war die Funktion der Polyphenoloxidasen (PPO) unklar. Inzwischen konnte aber gezeigt werden, dass eine Tyrosinase an der Betacyan‐Biosynthese des Portulakröschens (siehe Bild) und der Roten Rübe sowie eine Chalkon‐spezifische PPO an der Auronbildung in gelben Löwenmaulblüten beteiligt ist.
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This page was last modified on 27 Jan 2025 .
