New versatile approach for high-throughput analysis using GC-MS
IPB scientist have developed a versatile high-throughput screening method that aims to accelerate catalyst discovery and development. In order to avoid long analysis time, the method combines gas chromatography-mass spectrometry (GC-MS) with the so-called MISER approach, in which several samples are injected into a chromatography system in quick succession.
MISER-GC-MS is especially suitable for screening enzyme libraries. Thereby, reaction products are quantified selectively to identify mutagenesis-generated enzyme variants with increased activity. The analysis of a 96-well microtiter plate is feasible in 60 minutes with MISER-GC-MS. The method is substrate-independent, since the reaction products do not need to be labeled. Additionally, subsequent data evaluation is facilitated by an R-script.
As proof of concept, the chemists screened different enzyme libraries. They identified promising and novel unspecific peroxygenase chimeras (UPOs) consisting of subunits from three different fungal UPO genes. The study will be published as a cover story in the ChemCatChem Journal.
Reference:
Anja Knorrscheidt, Pascal Püllmann, Eugen Schell, Dominik Homann, Erik Freier and Martin J. Weissenborn. Identification of novel unspecific peroxygenase chimeras and unusual YfeX axial heme ligand by a versatile high-throughput GCMS approach. ChemCatChem DOI: 10.1002/cctc.202000618
