The IPB has once again been recognized for its exemplary actions in terms of equal opportunity-oriented personnel and organizational policies and has received the TOTAL E-QUALITY certification for the…
The Plant Science Student Conference (PSSC) has been organised by students from the two Leibniz institutes, IPK and IPB, every year for the last 20 years. In this interview, Christina Wäsch (IPK) and…
Mass spectrometry-based metabolomics approaches can enable detection and
quantification of many thousands of metabolite features simultaneously.
However, compound identification and reliable quantification are
greatly complicated owing to the chemical complexity and dynamic range
of the metabolome. Simultaneous quantification of many metabolites
within complex mixtures can additionally be complicated by ion
suppression, fragmentation and the presence of isomers. Here we present
guidelines covering sample preparation, replication and randomization,
quantification, recovery and recombination, ion suppression and peak
misidentification, as a means to enable high-quality reporting of liquid
chromatography– and gas chromatography-mass spectrometry-based
metabolomics-derived data.