The IPB has once again been recognized for its exemplary actions in terms of equal opportunity-oriented personnel and organizational policies and has received the TOTAL E-QUALITY certification for the…
The Plant Science Student Conference (PSSC) has been organised by students from the two Leibniz institutes, IPK and IPB, every year for the last 20 years. In this interview, Christina Wäsch (IPK) and…
Knorrscheidt, A.; Soler, J.; Hünecke, N.; Püllmann, P.; Garcia-Borràs, M.; Weissenborn, M. J.;Simultaneous screening of multiple substrates with an unspecific peroxygenase enabled modified alkane and alkene oxyfunctionalisationsCatal. Sci. Technol.116058(2021)DOI: 10.1039/d0cy02457k
A high throughput GC-MS approach was developed, permitting the
simultaneous analysis of up to three substrates and six products
quantitatively from one reaction mixture. This screening approach was
applied to site-saturation libraries of the novel unspecific
peroxygenase MthUPO. Using this setup enabled substantial
insights from a small mutant library. Enzyme variants were identified
exhibiting selective alkene epoxidation and substantially shifted
regioselectivities to 2- and 1-octanol formations. Computational
modelling rationalised the observed selectivity changes.