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IPB Newsletter | April 2025
Understanding Plants!

The IPB Video
Molecular Signal Processing

Prof. Dr. Steffen Abel
How plants stand their ground.
Bioorganic Chemistry

Prof. Dr. Ludger Wessjohann
The chemistry of nature - basis for active compounds for human and plant health.
Biochemistry of Plant Interactions

Prof. Dr. Tina Romeis
Interactions in, of and with plants.
Cell and Metabolic Biology

Prof. Dr. Alain Tissier
Secondary but never unimportant - the biological functions of plant secondary metabolites.
Independent Junior Research Group

Dr. Mariana Schuster

The IPB has once again been recognized for its exemplary actions in terms of equal opportunity-oriented personnel and organizational policies and has received the TOTAL E-QUALITY certification for the…

The Plant Science Student Conference (PSSC) has been organised by students from the two Leibniz institutes, IPK and IPB, every year for the last 20 years. In this interview, Christina Wäsch (IPK) and…

Over 600 guests came to the IPB on July 4 for the Long Night of Sciences to learn lots of new things and put their knowledge to the test at our science quiz course. This year, our program was aimed…

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PhD position in biology (m/f/d) (17/2025)

Date: 2025-08-08 Reference Number: 17/2025

PostDoc Researcher for Metabolomic and Proteomic Analysis (m/f/d) (16/2025)

Date: 2025-07-28 Reference Number: 16/2025

Technical Assistant or Laboratory Technician (m/f/d) (15/2025)

Date: 2025-07-25 Reference Number: 15/2025

PhD position in (bio)chemistry (m/f/d) (14/2025)

Date: 2025-07-11 Reference Number: 14/2025

PhD in Machine Learning for Enzyme Design (m/f/d)

Date: 2025-05-16 Reference Number: 11/2025

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Preprints

Rajaraman, J.; Douchkov, D.; Lück, S.; Hensel, G.; Nowara, D.; Pogoda, M.; Rutten, T.; Meitzel, T.; Höfle, C.; Hückelhoven, R.; Klinkenberg, J.; Trujillo, M.; Bauer, E.; Schmutzer, T.; Himmelbach, A.; Mascher, M.; Lazzari, B.; Stein, N.; Kumlehn, J.; Schweizer, P.; The partial duplication of an E3-ligase gene in Triticeae species mediates resistance to powdery mildew fungi bioRxiv (2017) DOI: 10.1101/190728

Preprints

Gantner, J.; Ilse, T.; Ordon, J.; Kretschmer, C.; Gruetzner, R.; Löfke, C.; Dagdas, Y.; Bürstenbinder, K.; Marillonnet, S.; Stuttmann, J.; Peripheral infrastructure vectors and an extended set of plant parts for the modular cloning system bioRxiv (2017) DOI: 10.1101/237768

Standardized DNA assembly strategies facilitate the generation of multigene constructs from collections of building blocks in plant synthetic biology. A common syntax for hierarchical DNA assembly following the Golden Gate principle employing Type IIs restriction endonucleases was recently developed, and underlies the Modular Cloning and GoldenBraid systems. In these systems, transcriptional units and/or multigene constructs are assembled from libraries of standardized building blocks, also referred to as phytobricks, in several hierarchical levels and by iterative Golden Gate reactions. This combinatorial assembly strategy meets the increasingly complex demands in biotechnology and bioengineering, and also represents a cost-efficient and versatile alternative to previous molecular cloning techniques. For Modular Cloning, a collection of commonly used Plant Parts was previously released together with the Modular Cloning toolkit itself, which largely facilitated the adoption of this cloning system in the research community. Here, a collection of approximately 80 additional phytobricks is provided. These phytobricks comprise e.g. modules for inducible expression systems, different promoters or epitope tags, which will increase the versatility of Modular Cloning-based DNA assemblies. Furthermore, first instances of a “peripheral infrastructure” around Modular Cloning are presented: While available toolkits are designed for the assembly of plant transformation constructs, vectors were created to also use coding sequence-containing phytobricks directly in yeast two hybrid interaction or bacterial infection assays. Additionally, DNA modules and assembly strategies for connecting Modular Cloning with Gateway Cloning are presented, which may serve as an interface between available resources and newly adopted hierarchical assembly strategies. The presented material will be provided as a toolkit to the plant research community and will further enhance the usefulness and versatility of Modular Cloning.

Preprints

Emami Khoonsari, P.; Moreno, P.; Bergmann, S.; Burman, J.; Capuccini, M.; Carone, M.; Cascante, M.; de Atauri, P.; Foguet, C.; Gonzalez-Beltran, A.; Hankemeier, T.; Haug, K.; He, S.; Herman, S.; Johnson, D.; Kale, N.; Larsson, A.; Neumann, S.; Peters, K.; Pireddu, L.; Rocca-Serra, P.; Roger, P.; Rueedi, R.; Ruttkies, C.; Sadawi, N.; Salek, R.; Sansone, S.-A.; Schober, D.; Selivanov, V.; Thévenot, E. A.; van Vliet, M.; Zanetti, G.; Steinbeck, C.; Kultima, K.; Spjuth, O.; Interoperable and scalable data analysis with microservices: Applications in Metabolomics bioRxiv (2017) DOI: 10.1101/213603

Preprints

Dissmeyer, N.; Rivas, S.; Graciet, E.; Life and death of proteins after protease cleavage: protein degradation by the N-end rule pathway bioRxiv (2017) DOI: 10.1101/115246

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IPB Newsletter | April 2025

Understanding Plants!

Molecular Signal Processing

Bioorganic Chemistry

Biochemistry of Plant Interactions

Cell and Metabolic Biology

Independent Junior Research Group

IPB receives TOTAL E-QUALITY certification for the sixth time in a row

PSSC: Success for the ‘Iron Lady’

Hands-on science and a big quiz marathon at the Long Night of Sciences

PhD position in biology (m/f/d) (17/2025)

PostDoc Researcher for Metabolomic and Proteomic Analysis (m/f/d) (16/2025)

Technical Assistant or Laboratory Technician (m/f/d) (15/2025)

PhD position in (bio)chemistry (m/f/d) (14/2025)

PhD in Machine Learning for Enzyme Design (m/f/d)

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