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Newsletter +++ April 2024
Leibniz Institute of Plant Biochemistry
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New CRISPR method allows stable knock-in of large genes
Major progress on the CRISPR front. IPB scientists have succeeded in stably and precisely inserting large gene segments into the genome of higher plants very efficiently. To do this, they optimized the CRISPR/Cas gene editing method. The improved CRISPR method offers great opportunities for the targeted modification of genes in higher plants, both for breeding and research. Published in Molecular Plant // >>Read more // >>IPB Press release
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| Arabidopsis F-box protein prolongs JA-based defense response. Commentary in Molecular Plant |
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| As highly respected jasmonate experts, Claus Wasternack and Bettina Hause recently had the honor of commenting on an article by Zhang et al. in the renowned journal Molecular Plant. The subject of the reviewed study is the Botrytis cinerea-induced F-box protein 1 (BFP1) in Arabidopsis, which is expressed upon infection with the gray mold pathogen and increases the plant's resistance to the pathogen by inhibiting jasmonic acid degradation. Published in Molecular Plant // >> Read more |
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| Multicolor pigments in tobacco |
| An efficient way to produce complex natural products is the transient expression of the enzymes involved in Nicotiana benthamiana. However, this does not apply to anthocyanins, which are poorly expressed in N. benthamiana. IPB scientists have now developed a benthamiana expression platform that can be used to produce any desired basic anthocyanin. Published in Plant Biotechnology Journal // >> Read more |
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| Out of the plant - into the yeast! Modern diterpene production through biotechnology |
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IPB scientists have recently generated a substantial library of diterpenoids by combinatorial biosynthesis. In a Golden Gate-based approach, they combined the catalytic activities of ten different diterpene synthases with four cytochrome P450 oxygenases from different plant species. The expression of all possible combinations in individual yeast strains led to the production of more than 200
diterpenoids, 162 of which are considered novel compounds. Published in Metabolic Engineering // >> Read more |
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| LOV-Protein-Engineering durch Machine Learning |
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| LOV photoreceptors control light-dependent processes such as phototropism in higher plants, but are also found in algae, fungi and bacteria. Modified variants of LOV proteins are used as reporter proteins and light sensors for experiments in biological systems. In an interdisciplinary team, IPB scientists have recently further optimized these photoreceptors using machine learning. Published in JACS Au // >> Read more |
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| Localization of the Arabidopsis FAD2 |
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| The proportion of unsaturated phospholipids in plant cell membranes plays an important role in their optimal function. In Arabidopsis, the oleoyl-Δ12-desaturase FAD2 ensures the desaturation of oleic acid. Until now, little was known about the distribution of the enzyme in the cell. MLU and IPB scientists have now determined the subcellular localization of fluorescent FAD2 fusions in Arabidopsis. Published in The Plant Journal // >> Read more |
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| Fascinating world of sesquiterpene lactones |
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| IPB scientists, together with Dutch and Canadian partners, have recently published a review on sesquiterpene lactones (STLs), in which they discuss in detail the biosynthesis, regulation and signaling function of these special natural products. Published in Critical Reviews in Plant Sciences // >> Read more |
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