Technische Ausstattung SZB
Metabolomics
LC-MS/MS
UPLC (Acquity, Waters) - QTrap 6500 (Sciex)
- targeted LC-MS/MS of
- central carbon metabolites
- phytohormones
- carotenoids
LDTD Ion Source
Laser Diode Thermal Desorption Ion Source (Phytronix)
- direct sample introduction source requiring no chromatographic separations prior to detection via MS
- e.g., for high-throughput screening of clone libraries
LC-MS
UPLC with absorbance, fluoresence and MS detection (Waters)
GC-MS
GCMS-QP2010 Ultra (Shimadzu)
- quadrupole-based GC-MS of volatile terpenoids
- thermo-desorption (TD-20, Shimadzu)
LC-MS/MS
UPLC (Acquity, Waters) - TripleToF 5600 (Sciex)
- untargeted LC-HR-SWATH-MS/MS of
- plant secondary metabolites
- hydrophilic/charged metabolites
Ion Chromatography System
Ion Chromatograph ICS-5000+ (Dionex, Thermo)
- cation exchange chromatography
- anion exchange chromatography
both coupled to
1. post-column ion suppression and
2. mass spectrometry
Online SPE-UPLC
Online solid phase extraction - UPLC (Symbiosis Pico, Spark Holland)
- sample clean-up
- ultra-sensitive phytohormone analysis
GC-MS
GC (TraceGC Ultra, Thermo) - MS (SQ, Thermo) with Atlas Injector
Proteinexpression und -aufreinigung
Fermenter
Bench-top bioreactor (Labfors 5, Infors HT) with Cleaning and sterilisation unit (LabCIP, Infors HT)
High pressure cell disruption
French Press TS 0.75 (Constant Systems)
FPLC
ÄKTA (GE Healthcare)
Expressionsanalysen
RNA Qualitätskontrolle
QIAxcel
MicroArray
Affymetrix GeneAtlas Personal MicroArray System
Real-Time PCR
BioRad Realtime System
Weitere Geräte
Mikroplattenreader TECAN
Mikrovolumen Spektrophotometer
