Schreiber, T.; Prange, A.; Schäfer, P.; Iwen, T.; Grützner, R.; Marillonnet, S.; Lepage, A.; Javelle, M.; Paul, W.; Tissier, A.; Efficient scar-free knock-ins of several kilobases in plants by engineered CRISPR/Cas endonucleases Mol. Plant (2024) DOI: 10.1016/j.molp.2024.03.013
Grützner, R.; König, K.; Horn, C.; Engler, C.; Laub, A.; Vogt, T.; Marillonnet, S.; A transient expression tool box for anthocyanin biosynthesis in Nicotiana benthamiana Plant Biotechnol. J. (2023) DOI: 10.1111/pbi.14261
Schreiber, T.; Tripathee, S.; Iwen, T.; Prange, A.; Vahabi, K.; Grützner, R.; Horn, C.; Marillonnet, S.; Tissier, A.; DNA double strand breaks lead to de novo transcription and translation of damage-induced long RNAs in planta bioRxiv (2022) DOI: 10.1101/2022.05.11.491484
Stellmach, H.; Hose, R.; Räde, A.; Marillonnet, S.; Hause, B.; A new set of Golden-Gate-Based organelle marker plasmids for colocalization studies in plants Plants 11, 2620, (2022) DOI: 10.3390/plants11192620
Yang, C.; Marillonnet, S.; Tissier, A.; The scarecrow-like transcription factor SlSCL3 regulates volatile terpene biosynthesis and glandular trichome size in tomato (Solanum lycopersicum) Plant J. 107, 1102-1118, (2021) DOI: 10.1111/tpj.15371
Stuttmann, J.; Barthel, K.; Martin, P.; Ordon, J.; Erickson, J. L.; Herr, R.; Ferik, F.; Kretschmer, C.; Berner, T.; Keilwagen, J.; Marillonnet, S.; Bonas, U.; Highly efficient multiplex editing: one‐shot generation of 8× Nicotiana benthamiana and 12× Arabidopsis mutants Plant J. 106, 8-22, (2021) DOI: 10.1111/tpj.15197
Grützner, R.; Martin, P.; Horn, C.; Mortensen, S.; Cram, E. J.; Lee-Parsons, C. W.; Stuttmann, J.; Marillonnet, S.; High-efficiency genome editing in plants mediated by a Cas9 gene containing multiple introns Plant Communications 2, 100135, (2021) DOI: 10.1016/j.xplc.2020.100135
Grützner, R.; Schubert, R.; Horn, C.; Yang, C.; Vogt, T.; Marillonnet, S.; Engineering Betalain Biosynthesis in Tomato for High Level Betanin Production in Fruits Front. Plant Sci. 12, 682443, (2021) DOI: 10.3389/fpls.2021.682443
Püllmann, P.; Knorrscheidt, A.; Münch, J.; Palme, P. R.; Hoehenwarter, W.; Marillonnet, S.; Alcalde, M.; Westermann, B.; Weissenborn, M. J.; A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases Commun. Biol. 4, 562, (2021) DOI: 10.1038/s42003-021-02076-3
Sheikh, A. H.; Fraz Hussain, R. M.; Tabassum, N.; Badmi, R.; Marillonnet, S.; Scheel, D.; Lee, J.; Sinha, A.; Possible role of WRKY transcription factors in regulating immunity in Oryza sativa ssp. indica Physiol. Mol. Plant Pathol. 114, 101623, (2021) DOI: 10.1016/j.pmpp.2021.101623
Püllmann, P.; Knorrscheidt, A.; Münch, J.; Palme, P. R.; Hoehenwarter, W.; Marillonnet, S.; Alcalde, M.; Westermann, B.; Weissenborn, M. J.; A modular two yeast species secretion system for the production and preparative application of fungal peroxygenases bioRxiv (2020) DOI: 10.1101/2020.07.22.216432
Grützner, R.; Martin, P.; Horn, C.; Mortensen, S.; Cram, E. J.; Lee-Parsons, C. W. T.; Stuttmann, J.; Marillonnet, S.; Addition of Multiple Introns to a Cas9 Gene Results in Dramatic Improvement in Efficiency for Generation of Gene Knockouts in Plants bioRxiv (2020) DOI: 10.1101/2020.04.03.023036
Barthel, K.; Martin, P.; Ordon, J.; Erickson, J. L.; Gantner, J.; Erickson, J. L.; Herr, R.; Kretschmer, C.; Ferik, F.; Berner, T.; Keilwagen, J.; Marillonnet, S.; Stuttmann, J.; Bonas, U.; One-shot generation of duodecuple (12x) mutant Arabidopsis: Highly efficient routine editing in model species bioRxiv (2020) DOI: 10.1101/2020.03.31.018671
Grunewald, S.; Marillonnet, S.; Hause, G.; Haferkamp, I.; Neuhaus, H. E.; Veß, A.; Hollemann, T.; Vogt, T.; The Tapetal Major Facilitator NPF2.8 is Required for Accumulation of Flavonol Glycosides on the Pollen Surface in Arabidopsis thaliana Plant Cell 32, 1727-1748, (2020) DOI: 10.1105/tpc.19.00801
Ordon, J.; Bressan, M.; Kretschmer, C.; Dall’Osto, L.; Marillonnet, S.; Bassi, R.; Stuttmann, J.; Optimized Cas9 expression systems for highly efficient Arabidopsis genome editing facilitate isolation of complex alleles in a single generation Funct. Integr. Genomics 20, 151-162, (2020) DOI: 10.1007/s10142-019-00665-4
Marillonnet, S.; Grützner, R.; Synthetic DNA Assembly Using Golden Gate Cloning and the Hierarchical Modular Cloning Pipeline Curr. Protoc. Mol. Biol. 130, e115, (2020) DOI: 10.1002/cpmb.115
Marillonnet, S.; Werner, S.; Assembly of Multigene Constructs Using the Modular Cloning System MoClo (In: Chandran S., George K.). Methods Mol. Biol. 2205, 125-141, (2020) ISBN: 978-1-0716-0907-1 DOI: 10.1007/978-1-0716-0908-8_8
Grützner, R.; Marillonnet, S.; Generation of MoClo Standard Parts Using Golden Gate Cloning (In: Chandran S., George K.). Methods Mol. Biol. 2205, 107-123, (2020) ISBN: 978-1-0716-0907-1 DOI: 10.1007/978-1-0716-0908-8_7
Schubert, R.; Dobritzsch, S.; Gruber, C.; Hause, G.; Athmer, B.; Schreiber, T.; Marillonnet, S.; Okabe, Y.; Ezura, H.; Acosta, I. F.; Tarkowská, D.; Hause, B.; Tomato MYB21 Acts in Ovules to Mediate Jasmonate-Regulated Fertility Plant Cell 31, 1043-1062, (2019) DOI: 10.1105/tpc.18.00978
Hausner, J.; Jordan, M.; Otten, C.; Marillonnet, S.; Büttner, D.; Modular Cloning of the Type III Secretion Gene Cluster from the Plant-Pathogenic Bacterium Xanthomonas euvesicatoria ACS Synth. Biol. 8, 532-547, (2019) DOI: 10.1021/acssynbio.8b00434
Püllmann, P.; Ulpinnis, C.; Marillonnet, S.; Gruetzner, R.; Neumann, S.; Weissenborn, M. J.; Golden Mutagenesis: An efficient multi-site-saturation mutagenesis approach by Golden Gate cloning with automated primer design Sci. Rep. 9, 10932, (2019) DOI: 10.1038/s41598-019-47376-1
Marillonnet, S.; Werner, S.; Assembly of Complex Pathways Using Type IIs Restriction Enzymes (Santos, C. N. S. & Ajikumar, P. K., eds.). Methods Mol. Biol. 1927, 93-109, (2019) ISBN: 978-1-4939-9142-6 DOI: 10.1007/978-1-4939-9142-6_7
Püllmann, P.; Ulpinnis, C.; Marillonnet, S.; Gruetzner, R.; Neumann, S.; Weissenborn, M. J.; Golden Mutagenesis: An efficient multi-site-saturation mutagenesis approach by Golden Gate cloning with automated primer design bioRxiv (2018) DOI: 10.1101/453621
Ordon, J.; Bressan, M.; Kretschmer, C.; Dall'Osto, L.; Marillonnet, S.; Bassi, R.; Stuttmann, J.; Optimized Cas9 expression systems for highly efficient Arabidopsis genome editing facilitate isolation of complex alleles in a single generation bioRxiv (2018) DOI: 10.1101/393439
Stauder, R.; Welsch, R.; Camagna, M.; Kohlen, W.; Balcke, G. U.; Tissier, A.; Walter, M. H.; Strigolactone Levels in Dicot Roots Are Determined by an Ancestral Symbiosis-Regulated Clade of the PHYTOENE SYNTHASE Gene Family Front. Plant Sci. 9, 255, (2018) DOI: 10.3389/fpls.2018.00255
Kowarschik, K.; Hoehenwarter, W.; Marillonnet, S.; Trujillo, M.; UbiGate: a synthetic biology toolbox to analyse ubiquitination New Phytol. 217, 1749-1763, (2018) DOI: 10.1111/nph.14900
Gantner, J.; Ordon, J.; Ilse, T.; Kretschmer, C.; Gruetzner, R.; Löfke, C.; Dagdas, Y.; Bürstenbinder, K.; Marillonnet, S.; Stuttmann, J.; Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system PLOS ONE 13, e0197185, (2018) DOI: 10.1371/journal.pone.0197185
Gantner, J.; Ilse, T.; Ordon, J.; Kretschmer, C.; Gruetzner, R.; Löfke, C.; Dagdas, Y.; Bürstenbinder, K.; Marillonnet, S.; Stuttmann, J.; Peripheral infrastructure vectors and an extended set of plant parts for the modular cloning system bioRxiv (2017) DOI: 10.1101/237768
Scheibner, F.; Marillonnet, S.; Büttner, D.; The TAL Effector AvrBs3 from Xanthomonas campestris pv. vesicatoria Contains Multiple Export Signals and Can Enter Plant Cells in the Absence of the Type III Secretion Translocon Front. Microbiol. 8, 2180, (2017) DOI: 10.3389/fmicb.2017.02180
Scheler, U.; Brandt, W.; Porzel, A.; Rothe, K.; Manzano, D.; Božić, D.; Papaefthimiou, D.; Balcke, G. U.; Henning, A.; Lohse, S.; Marillonnet, S.; Kanellis, A. K.; Ferrer, A.; Tissier, A.; Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast Nat. Commun. 7, 12942, (2016) DOI: 10.1038/ncomms12942
Scheibner, F.; Schulz, S.; Hausner, J.; Marillonnet, S.; Büttner, D.; Type III-Dependent Translocation of HrpB2 by a Nonpathogenic hpaABC Mutant of the Plant-Pathogenic Bacterium Xanthomonas campestris pv. vesicatoria Appl. Environ. Microbiol. 82, 3331-3347, (2016) DOI: 10.1128/AEM.00537-16
Dobritzsch, M.; Lübken, T.; Eschen-Lippold, L.; Gorzolka, K.; Blum, E.; Matern, A.; Marillonnet, S.; Böttcher, C.; Dräger, B.; Rosahl, S.; MATE Transporter-Dependent Export of Hydroxycinnamic Acid Amides Plant Cell 28, 583-596, (2016) DOI: 10.1105/tpc.15.00706
Brückner, K.; Schäfer, P.; Weber, E.; Grützner, R.; Marillonnet, S.; Tissier, A.; A library of synthetic transcription activator-like effector-activated promoters for coordinated orthogonal gene expression in plants Plant J. 82, 707-716, (2015) DOI: 10.1111/tpj.12843
Patron, N. J.; Orzaez, D.; Marillonnet, S.; Warzecha, H.; Matthewman, C.; Youles, M.; Raitskin, O.; Leveau, A.; Farré, G.; Rogers, C.; Smith, A.; Hibberd, J.; Webb, A. A. R.; Locke, J.; Schornack, S.; Ajioka, J.; Baulcombe, D. C.; Zipfel, C.; Kamoun, S.; Jones, J. D. G.; Kuhn, H.; Robatzek, S.; Van Esse, H. P.; Sanders, D.; Oldroyd, G.; Martin, C.; Field, R.; O'Connor, S.; Fox, S.; Wulff, B.; Miller, B.; Breakspear, A.; Radhakrishnan, G.; Delaux, P.-M.; Loqué, D.; Granell, A.; Tissier, A.; Shih, P.; Brutnell, T. P.; Quick, W. P.; Rischer, H.; Fraser, P. D.; Aharoni, A.; Raines, C.; South, P. F.; Ané, J.-M.; Hamberger, B. R.; Langdale, J.; Stougaard, J.; Bouwmeester, H.; Udvardi, M.; Murray, J. A. H.; Ntoukakis, V.; Schäfer, P.; Denby, K.; Edwards, K. J.; Osbourn, A.; Haseloff, J.; Standards for plant synthetic biology: a common syntax for exchange of DNA parts New Phytol. 208, 13-19, (2015) DOI: 10.1111/nph.13532
Walter, M. H.; Stauder, R.; Tissier, A.; Evolution of root-specific carotenoid precursor pathways for apocarotenoid signal biogenesis Plant Sci. 233, 1-10, (2015) DOI: 10.1016/j.plantsci.2014.12.017
Heinze, M.; Brandt, W.; Marillonnet, S.; Roos, W.; “Self” and “Non-Self” in the Control of Phytoalexin Biosynthesis: Plant Phospholipases A2 with Alkaloid-Specific Molecular Fingerprints Plant Cell 27, 448-462, (2015) DOI: 10.1105/tpc.114.135343
Marillonnet, S.; Werner, S.; Assembly of Multigene Constructs Using Golden Gate Cloning (Castilho, A., ed.). Methods Mol. Biol. 1321, 269-284, (2015) ISBN: 978-1-4939-2760-9 DOI: 10.1007/978-1-4939-2760-9_19
Schneider, J. D.; Marillonnet, S.; Castilho, A.; Gruber, C.; Werner, S.; Mach, L.; Klimyuk, V.; Mor, T. S.; Steinkellner, H.; Oligomerization status influences subcellular deposition and glycosylation of recombinant butyrylcholinesterase in Nicotiana benthamiana Plant Biotechnol. J. 12, 832-839, (2014) DOI: 10.1111/pbi.12184
Engler, C.; Youles, M.; Gruetzner, R.; Ehnert, T.-M.; Werner, S.; Jones, J. D. G.; Patron, N. J.; Marillonnet, S.; A Golden Gate Modular Cloning Toolbox for Plants ACS Synth. Biol. 3, 839-843, (2014) DOI: 10.1021/sb4001504
Thieme, F.; Marillonnet, S.; Quick and Clean Cloning (Valla, S. & Lale, R., eds.). Methods Mol. Biol. 1116, 37-48, (2014) ISBN: 978-1-62703-764-8 DOI: 10.1007/978-1-62703-764-8_3
Engler, C.; Marillonnet, S.; Golden Gate Cloning (Valla, S. & Lale, R., eds.). Methods Mol. Biol. 1116, 119-131, (2014) ISBN: 978-1-62703-764-8 DOI: 10.1007/978-1-62703-764-8_9
Engler, C.; Marillonnet, S.; Combinatorial DNA Assembly Using Golden Gate Cloning (Polizzi, K. M. & Kontoravdi, C., eds.). Methods Mol. Biol. 1073, 141-156, (2013) ISBN: 978-1-62703-625-2 DOI: 10.1007/978-1-62703-625-2_12
Werner, S.; Engler, C.; Weber, E.; Gruetzner, R.; Marillonnet, S.; Fast track assembly of multigene constructs using Golden Gate cloning and the MoClo system Bioengineered 3, 38-43, (2012) DOI: 10.4161/bbug.3.1.18223
Weber, E.; Gruetzner, R.; Werner, S.; Engler, C.; Marillonnet, S.; Assembly of Designer TAL Effectors by Golden Gate Cloning PLOS ONE 6, e19722, (2011) DOI: 10.1371/journal.pone.0019722
Weber, E.; Engler, C.; Gruetzner, R.; Werner, S.; Marillonnet, S.; A Modular Cloning System for Standardized Assembly of Multigene Constructs PLOS ONE 6, e16765, (2011) DOI: 10.1371/journal.pone.0016765
Thieme, F.; Engler, C.; Kandzia, R.; Marillonnet, S.; Quick and Clean Cloning: A Ligation-Independent Cloning Strategy for Selective Cloning of Specific PCR Products from Non-Specific Mixes PLOS ONE 6, e20556, (2011) DOI: 10.1371/journal.pone.0020556
Engler, C.; Marillonnet, S.; Generation of Families of Construct Variants Using Golden Gate Shuffling (Lu, C. et al., eds.). Methods Mol. Biol. 729, 167-181, (2011) ISBN: 978-1-61779-065-2 DOI: 10.1007/978-1-61779-065-2_11
Engler, C.; Gruetzner, R.; Kandzia, R.; Marillonnet, S.; Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes PLOS ONE 4, e5553, (2009) DOI: 10.1371/journal.pone.0005553
Engler, C.; Kandzia, R.; Marillonnet, S.; A One Pot, One Step, Precision Cloning Method with High Throughput Capability PLOS ONE 3, e3647, (2008) DOI: 10.1371/journal.pone.0003647