@INBOOK{IPB-2616, author = {Jozefowicz, A. M. and Döll, S. and Mock, H.-P.}, title = {{Plant Cold Acclimation}}, year = {2020}, pages = {161-170}, chapter = {{Proteomic Approaches to Identify Proteins Responsive to Cold Stress}}, journal = {Methods Mol Biol}, editor = {Hincha, D. K. \& Zuther, E., eds.}, doi = {10.1007/978-1-0716-0660-5_12}, url = {https://dx.doi.org/10.1007/978-1-0716-0660-5_12}, volume = {2156}, abstract = {Changing environmental conditions greatly affect the accumulation of many proteins; therefore, the analysis of alterations in the proteome is essential to understand the plant response to abiotic stress. Proteomics provides a platform for the identification and quantification of plant proteins responsive to cold stress and taking part in cold acclimation. Here, we describe the preparation of proteins for LC-MS measurement to monitor the changes of protein patterns during cold treatment in Arabidopsis thaliana. In our protocol, proteins are precipitated using TCA/acetone, quantified with 2D Quant Kit and digested with trypsin using a filter-based method and analyzed using an LC-MS approach. The acquired results can be further applied for label-free protein quantification.} }