@Article{IPB-2326, author = {Schreiber, T. and Prange, A. and Hoppe, T. and Tissier, A.}, title = {{Split-TALE: A TALE-Based Two-Component System for Synthetic Biology Applications in Planta}}, year = {2019}, pages = {1001-1012}, journal = {Plant Physiol}, doi = {10.1104/pp.18.01218}, url = {https://dx.doi.org/10.1104/pp.18.01218}, volume = {179}, abstract = {Transcription activator-like effectors (TALEs) are bacterial Type-III effector proteins from phytopathogenic Xanthomonas species that act as transcription factors in plants. The modular DNA-binding domain of TALEs can be reprogrammed to target nearly any DNA sequence. Here, we designed and optimized a two-component AND-gate system for synthetic circuits in plants based on TALEs. In this system, named split-TALE (sTALE), the TALE DNA binding domain and the transcription activation domain are separated and each fused to protein interacting domains. Physical interaction of interacting domains leads to TALE-reconstitution and can be monitored by reporter gene induction. This setup was used for optimization of the sTALE scaffolds, which result in an AND-gate system with an improved signal-to-noise ratio. We also provide a toolkit of ready-to-use vectors and single modules compatible with Golden Gate cloning and MoClo syntax. In addition to its implementation in synthetic regulatory circuits, the sTALE system allows the analysis of protein-protein interactions in planta.} }