@Article{IPB-1561,
author = {Jindaprasert, A. and Samappito, S. and Springob, K. and Schmidt, J. and Gulder, T. and De-Eknamkul, W. and Bringmann, G. and Kutchan, T. M.},
title = {{In Vitro Plants, Callus and Root Cultures of Plumbago indica and Their Biosynthetic Potential for Plumbagin}},
year = {2010},
pages = {53-65},
journal = {King Mongkut\'s Agro-Industry Journal},
volume = {2},
abstract = {In vitro cultured plants of Plumbago indica L. were established from nodal segments and micropropagated on hormone-free LS medium. These in vitro plantlets produced plumbagin with the content 0.79-0.87 mg g-1 dry weight which was more than half of the content found in the whole roots of greenhouse plants. Root and callus cultures were also initiated from stem and young leaf explants, respectively. The root cultures maintained in hormone-free MS medium accumulated 0.28 mg g-1 plumbagin whereas the callus cultures grown on MS medium supplemented with 1.0 mg l-1 2,4-dichloropenoxyacetic acid (2,4-D) and 0.1 mg l-1 kinetin contained only 0.013 mg g-1 of the compound. In addition to plumbagin, its related compounds plumbagic acid and plumbagic acid glucoside were also found specifically in the root tissues of the micropropagated plantlets and the root cultures. These results suggested the biosynthetic potential for the plumbagin-derived compounds in the tissues of in vitro plants and organ cultures which allows us to use them as materials for studying genes and enzymes involved in the naphthoquinone formation in P. indica.}    
}