@INBOOK{IPB-21, author = {Poeschl, Y. and Möller, B. and Müller, L. and Bürstenbinder, K. and}, year = {2020}, pages = {349-363}, chapter = {{User-friendly assessment of pavement cell shape features with PaCeQuant: Novel functions and tools}}, journal = {Methods Cell Biol.}, editor = {Charles T. Anderson, Elizabeth S. Haswell, Ram Dixit}, doi = {10.1016/bs.mcb.2020.04.010}, url = {https://doi.org/10.1016/bs.mcb.2020.04.010}, volume = {160}, abstract = {Leaf epidermis pavement cells develop complex jigsaw puzzle-like shapes in many plant species, including the model plant Arabidopsis thaliana. Due to their complex morphology, pavement cells have become a popular model system to study shape formation and coordination of growth in the context of mechanically coupled cells at the tissue level. To facilitate robust assessment and analysis of pavement cell shape characteristics in a high-throughput fashion, we have developed PaCeQuant and a collection of supplemental tools. The ImageJ-based MiToBo plugin PaCeQuant supports fully automatic segmentation of cell contours from microscopy images and the extraction of 28 shape features for each detected cell. These features now also include the Largest Empty Circle criterion as a proxy for mechanical stress. In addition, PaCeQuant provides a set of eight features for individual lobes, including the categorization as type I and type II lobes at two- and three-cell junctions, respectively. The segmentation and feature extraction results of PaCeQuant depend on the quality of input images. To allow for corrections in case of local segmentation errors, the LabelImageEditor is provided for user-friendly manual postprocessing of segmentation results. For statistical analysis and visualization, PaCeQuant is supplemented with the R package PaCeQuantAna, which provides statistical analysis functions and supports the generation of publication-ready plots in ready-to-use R workflows. In addition, we recently released the FeatureColorMapper tool which overlays feature values over cell regions for user-friendly visual exploration of selected features in a set of analyzed cells.} } @INBOOK{IPB-20, author = {Mielke, S. and Gasperini, D. and}, title = {{Jasmonate in Plant Biology}}, year = {2020}, pages = {69-78}, chapter = {{Plant–Insect Bioassay for Testing Arabidopsis Resistance to the Generalist Herbivore Spodoptera littoralis}}, journal = {Methods Mol. Biol.}, editor = {Champion, A. \& Laplaze, L., eds.}, doi = {10.1007/978-1-0716-0142-6_5}, volume = {2085}, abstract = {Jasmonates are essential engineers of plant defense responses against many pests, including herbivorous insects. Herbivory induces the production of jasmonic acid (JA) and its bioactive conjugate jasmonoyl-l-isoleucine (JA-Ile), which then triggers a large transcriptional reprogramming to promote plant acclimation. The contribution of the JA pathway, including its components and regulators, to defense responses against insect herbivory can be evaluated by conducting bioassays with a wide range of host plants and insect pests. Here, we describe a detailed and reproducible protocol for testing feeding behavior of the generalist herbivore Spodoptera littoralis on the model plant Arabidopsis thaliana and hence infer the contribution of JA-mediated plant defense responses to a chewing insect.} } @INBOOK{IPB-85, author = {Wasternack, C. and Hause, B. and}, title = {{Festkolloquium der Leopoldina anlässlich des 80. Geburtstages von Herrn Altpräsidenten Benno Parthier}}, year = {2013}, pages = {29-38}, chapter = {{Benno Parthier und die Jasmonatforschung in Halle}}, journal = {Nova Acta Leopoldina}, editor = {Hacker, J., ed.}, url = {https://www.leopoldina.org/publikationen/detailansicht/publication/festkolloquium-der-leopoldina-anlaesslich-des-80-geburtstages-von-herrn-altpraesidenten-benno-parthie/}, volume = {Supplementum Nr. 28}, } @INBOOK{IPB-112, author = {Flores, R. and Carbonell, A. and De la Peña, M. and Gago, S. and}, title = {{Herramientas Biotecnológicas en Fitopatología}}, year = {2007}, pages = {407-425}, chapter = {{RNAs Autocatalíticos: Ribozimas de Cabeza de Martillo}}, } @INBOOK{IPB-118, author = {Wasternack, C. and Hause, B. and Stenzel, I. and Goetz, S. and Feussner, I. and Miersch, O. and}, title = {{Proceeding of the 17th Int. Symp. on Plant Lipids}}, year = {2007}, pages = {107-111}, chapter = {{Jasmonate signaling in tomato – The input of tissue-specific occurrence of allene oxide cyclase and JA metabolites}}, editor = {Benning C., Ollrogge, J.}, }