Publikationen - Molekulare Signalverarbeitung
Aktive Filter
Suchfilter
- Typ der Publikation
- Publikation (1)
- Erscheinungsjahr
- Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert
- Virus Res. (1)
- Autor Nach Häufigkeit alphabetisch sortiert
- Wasternack, C. (9)
- Quint, M. (7)
- Hause, B. (6)
- Miersch, O. (5)
- Abel, S. (3)
- Gago, S. (3)
- Bürstenbinder, K. (2)
- Carbonell, A. (2)
- Delker, C. (2)
- Feussner, I. (2)
- Flores, R. (2)
- Gray, W. M. (2)
- Grosse, I. (2)
- Huang, H. (2)
- Lange, P. R. (2)
- Martínez de Alba, A.-E. (2)
- Müller, J. (2)
- Schmidt, J. (2)
- Stenzel, I. (2)
- Acosta, I. F. (1)
- Adamson, A. W. (1)
- Alonso, J. (1)
- Bartsch, M. (1)
- Bassel, G. W. (1)
- Bentsink, L. (1)
- Blée, E. (1)
- Brandt, W. (1)
- Brüx, A. (1)
- Böttcher, C. (1)
- Chaâbene, Z. (1)
- Chételat, A. (1)
- Costa, N. (1)
- De-Eknamkul, W. (1)
- Dekkers, B. J. (1)
- Delgado, S. (1)
- Demuth, H.-U. (1)
- Di Serio, F. (1)
- Dinesh, D. C. (1)
- Dippe, M. (1)
- Domergue, F. (1)
- Drira, N. (1)
- Drost, H.-G. (1)
- Duran-Vila, N. (1)
- Elleuch, A. (1)
- Farmer, E. E. (1)
- Fellenberg, C. (1)
- Floß, D. S. (1)
- Forner, S. (1)
- Gago-Zachert, S. P. (1)
- Gao, X. (1)
- Gasperini, D. (1)
- Geserick, C. (1)
- Gilbert, J. (1)
- Grau, O. (1)
- Grubb, C. D. (1)
- Grubb, D. C. (1)
- Holdsworth, M. J. (1)
- Huard, C. (1)
- Iglesias, N. G. (1)
- Ito, H. (1)
- Jindaprasert, A. (1)
- Joubès, J. (1)
- Khemakhem, B. (1)
- Kienow, L. (1)
- King, J. R. (1)
- Kohlschmidt, J. (1)
- Kolomiets, M. (1)
- Kombrink, E. (1)
- Kopycki, J. (1)
- Krasnogor, N. (1)
- Krebs, M. (1)
- Kutchan, T. M. (1)
- Kwong, R. (1)
- Küster, H. (1)
- Lange, P.-R. (1)
- Leubner-Metzger, G. (1)
- Liu, T.-Y. (1)
- Lohmann, J. U. (1)
- Léger, A. (1)
- Lübberstedt, T. (1)
- Marshall, A. (1)
- Mejdoub, H. (1)
- Milkowski, C. (1)
- Mongrand, S. (1)
- Müller, K. (1)
- Navarro-Quezada, A. (1)
- Neumerkel, J. (1)
- Noel, L. D. (1)
- Oka, M. (1)
- Pearce, S. (1)
- Pedras, M. S. (1)
- Plata, M. I. (1)
- Poeschl, Y. (1)
- Proels, R. (1)
- Raffaele, S. (1)
- Raschke, A. (1)
- Robledo, G. (1)
- Roby, D. (1)
- Rodio, M.-E. (1)
- Roitsch, T. (1)
Zeige Ergebnisse 1 bis 1 von 1.
Serra, P.; Gago, S.; Duran-Vila, N.; A single nucleotide change in Hop stunt viroid modulates citrus cachexia symptoms Virus Res. 138, 130-134, (2008) DOI: 10.1016/j.virusres.2008.08.003
Cachexia disease of citrus is caused by Hop stunt viroid (HSVd). In citrus, pathogenic and non-pathogenic strains differ by a “cachexia expression motif” of five to six nucleotides located in the variable domain of the proposed rod-like secondary structure. Here, site-directed mutants were generated to investigate if all these nucleotides were required for infectivity and/or symptom expression. Specifically an artificial cachexia inducing mutant M0 was generated by introducing the six nucleotides changes of the “cachexia expression motif” into a non-pathogenic sequence variant and M0 was used as a template to systematically restore some of the introduced changes. The resulting mutants in which specific changes introduced to generate M0, were restored presented a variety of responses: (i) M1, obtained by introducing two insertions forming a base-pair, was infectious but non-pathogenic; (ii) M2, obtained by introducing an insertion and restoring a substitution, presented low infectivity and the resulting progeny reverted to M0; (iii) M3, obtained by restoring a single substitution in the lower strand of the viroid secondary structure, was infectious but induced only mild cachexia symptoms; (iv) M4, obtained by restoring a single susbtitution in the upper strand of the viroid secondary structure, was non-infectious. These results confirm that the “cachexia expression motif” plays a major role in inciting cachexia symptoms, and that subtle changes within this motif affect symptom severity and may even suppress symptom expression.