@Article{IPB-1725, author = {Ziegler, J. and Qwegwer, J. and Schubert, M. and Erickson, J.L. and Schattat, M. and Bürstenbinder, K. and Grubb, C.D. and Abel, S.}, title = {{Simultaneous analysis of apolar phytohormones and 1-aminocyclopropan-1-carboxylic acid by high performance liquid chromatography/electrospray negative ion tandem mass spectrometry via 9-fluorenylmethoxycarbonyl chloride derivatization}}, year = {2014}, pages = {102-109}, journal = {J Chromatogr A}, doi = {10.1016/j.chroma.2014.08.029}, url = {http://www.sciencedirect.com/science/article/pii/S002196731401259X}, volume = {1362}, abstract = {A strategy to detect and quantify the polar ethylene precursor 1-aminocyclopropan-1-carboxylic acid (ACC) along with the more apolar phytohormones abscisic acid (ABA), indole-3-acetic acid (IAA), jasmonic acid (JA), jasmonic acid-isoleucine conjugate (JA-Ile), 12-oxo-phytodienoic acid (OPDA), trans-zeatin, and trans-zeatin 9-riboside using a single extraction is presented. Solid phase resins commonly employed for extraction of phytohormones do not allow the recovery of ACC. We circumvent this problem by attaching an apolar group to ACC via derivatization with the amino group specific reagent 9-fluorenylmethoxycarbonyl chloride (Fmoc-Cl). Derivatization in the methanolic crude extract does not modify other phytohormones. The derivatized ACC could be purified and detected together with the more apolar phytohormones using common solid phase extraction resins and reverse phase HPLC/electrospray negative ion tandem mass spectrometry. The limit of detection was in the low nanomolar range for all phytohormones, a sensitivity sufficient to accurately determine the phytohormone levels from less than 50 mg (fresh weight) of Arabidopsis thaliana and Nicotiana benthamiana tissues. Comparison with previously published phytohormone levels and the reported changes in phytohormone levels after stress treatments confirmed the accuracy of the method.} } @Article{IPB-699, author = {Sharma, V.K. and Monostori, T. and Hause, B. and Maucher, H. and Göbel, C. and Hornung, E. and Hänsch, R. and Bittner, F. and Wasternack, C. and Feussner, I. and Mendel, R.R. and Schulze, J.}, title = {{Genetic transformation of barley to modify expression of a 13-lipoxygenase}}, year = {2005}, pages = {33-34 }, journal = {Acta Biol. Szeged }, url = {http://www2.sci.u-szeged.hu/ABS/2005/Acta%20HP/4933.pdf}, volume = {49}, abstract = {Immature scutella of barley were transformed with cDNA coding for a 13-li-poxygenase of barley (LOX-100) via particle bombardment. Regenerated plants were tested by PAT-assay, Western-analysis and PCR-screening. Immunocytochemical assay of T0 plants showed expression of the LOX cDNA both in the chloroplasts and in the cytosol, depending on the presence of the chloroplast signal peptide sequences in the cDNA. A few transgenic plants containing higher amounts of LOX-derived products have been found. These are the candidates for further analysis concerning pathogen resistance.} }