Hamdi, I.; Elleuch, A.; Bessaies, N.; Grubb, C. D.; Fakhfakh, H. First report of Citrus viroid V in North Africa J Gen Plant Pathol 81, 87-91, (2015) DOI: 10.1007/s10327-014-0556-9
We tested citrus samples from Tunisia using
reverse transcription-polymerase chain reaction (RT-PCR), and for the
first time, Citrus viroid V (CVd-V) was reported in North Africa.
Fourteen of 38 tested citrus trees were infected by CVd-V including the
majority of varieties grown in Tunisia. Some RT-PCR results were also
supported by biological indexing. After sequencing the RT-PCR products,
three new CVd-V variants were identified, showing 80–91 % nucleotide
sequence identity with those reported previously. Based on phylogenetic
analysis using all CVd-V sequences in GenBank, two main CVd-V groups
were identified. Furthermore, construction of a genetic network of the
detected haplotypes using the same sequences shows a clear geographical
structuring of Tunisian CVd-V variants.