TY - JOUR ID - 1851 TI - A previously undescribed jasmonate compound in flowering Arabidopsis thaliana – The identification of cis-(+)-OPDA-Ile. JO - Phytochemistry PY - 2016 SP - 230-237 AU - Floková, K. AU - Feussner, K. AU - Herrfurth, C. AU - Miersch, O. AU - Mik, V. AU - Tarkowská, D. AU - Strnad, M. AU - Feussner, I. AU - Wasternack, C. AU - Novák, O. VL - 122 UR - DO - 10.1016/j.phytochem.2015.11.012 AB - Jasmonates (JAs) are plant hormones that integrate external stress stimuli with physiological responses. (+)-7-iso-JA-L-Ile is the natural JA ligand of COI1, a component of a known JA receptor. The upstream JA biosynthetic precursor cis-(+)-12-oxo-phytodienoic acid (cis-(+)-OPDA) has been reported to act independently of COI1 as an essential signal in several stress-induced and developmental processes. Wound-induced increases in the endogenous levels of JA/JA-Ile are accompanied by two to tenfold increases in the concentration of OPDA, but its means of perception and metabolism are unknown. To screen for putative OPDA metabolites, vegetative tissues of flowering Arabidopsis thaliana were extracted with 25% aqueous methanol (v/v), purified by single-step reversed-phase polymer-based solid-phase extraction, and analyzed by high throughput mass spectrometry. This enabled the detection and quantitation of a low abundant OPDA analog of the biologically active (+)-7-iso-JA-L-Ile in plant tissue samples. Levels of the newly identified compound and the related phytohormones JA, JA-Ile and cis-(+)-OPDA were monitored in wounded leaves of flowering Arabidopsis lines (Col-0 and Ws) and compared to the levels observed in Arabidopsis mutants deficient in the biosynthesis of JA (dde2-2, opr3) and JA-Ile (jar1). The observed cis-(+)-OPDA-Ile levels varied widely, raising questions concerning its role in Arabidopsis stress responses. A2 - C1 - ER - TY - JOUR ID - 1650 TI - UHPLC-MS/MS based target profiling of stress-induced phytohormones JO - Phytochemistry PY - 2014 SP - 147-157 AU - Floková, K. AU - Tarkowská, D. AU - Miersch, O. AU - Strnad, M. AU - Wasternack, C. AU - Novak, O. VL - 105 UR - http://www.sciencedirect.com/science/journal/00319422 DO - 10.1016/j.phytochem.2014.05.015 AB - Stress-induced changes in phytohormone metabolite profiles have rapid effects on plant metabolic activity and growth. The jasmonates (JAs) are a group of fatty acid-derived stress response regulators with roles in numerous developmental processes. To elucidate their dual regulatory effects, which overlap with those of other important defence-signalling plant hormones such as salicylic acid (SA), abscisic acid (ABA) and indole-3-acetic acid (IAA), we have developed a highly efficient single-step clean-up procedure for their enrichment from complex plant matrices that enables their sensitive quantitative analysis using hyphenated mass spectrometry technique. The rapid extraction of minute quantities of plant material (less than 20 mg fresh weight, FW) into cold 10% methanol followed by one-step reversed-phase polymer-based solid phase extraction significantly reduced matrix effects and increased the recovery of labile JA analytes. This extraction and purification protocol was paired with a highly sensitive and validated ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method and used to simultaneously profile sixteen stress-induced phytohormones in minute plant material samples, including endogenous JA, several of its biosynthetic precursors and derivatives, as well as SA, ABA and IAA. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 1378 TI - Role of cis-12-oxo-phytodienoic acid in tomato embryo development. JO - Plant Physiol PY - 2012 SP - 1715-1727 AU - Goetz, S. AU - Hellwege, A. AU - Stenzel, I. AU - Kutter, C. AU - Hauptmann, V. AU - Forner, S. AU - McCaig, B. AU - Hause, G. AU - Miersch, O. AU - Wasternack, C. AU - Hause, B. VL - 158 UR - https://dx.doi.org/10.1104/pp.111.192658 DO - 10.1104/pp.111.192658 AB - Oxylipins including jasmonates are signaling compounds in plant growth, development, and responses to biotic and abiotic stresses. In Arabidopsis (Arabidopsis thaliana) most mutants affected in jasmonic acid (JA) biosynthesis and signaling are male sterile, whereas the JA-insensitive tomato (Solanum lycopersicum) mutant jai1 is female sterile. The diminished seed formation in jai1 together with the ovule-specific accumulation of the JA biosynthesis enzyme allene oxide cyclase (AOC), which correlates with elevated levels of JAs, suggest a role of oxylipins in tomato flower/seed development. Here, we show that 35S::SlAOC-RNAi lines with strongly reduced AOC in ovules exhibited reduced seed set similarly to the jai1 plants. Investigation of embryo development of wild-type tomato plants showed preferential occurrence of AOC promoter activity and AOC protein accumulation in the developing seed coat and the embryo, whereas 12-oxo-phytodienoic acid (OPDA) was the dominant oxylipin occurring nearly exclusively in the seed coat tissues. The OPDA- and JA-deficient mutant spr2 was delayed in embryo development and showed an increased programmed cell death in the developing seed coat and endosperm. In contrast, the mutant acx1a, which accumulates preferentially OPDA and residual amount of JA, developed embryos similar to the wild type, suggesting a role of OPDA in embryo development. Activity of the residual amount of JA in the acx1a mutant is highly improbable since the known reproductive phenotype of the JA-insensitive mutant jai1 could be rescued by wound-induced formation of OPDA. These data suggest a role of OPDA or an OPDA-related compound for proper embryo development possibly by regulating carbohydrate supply and detoxification. KW - Cell Biology A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER - TY - JOUR ID - 1437 TI - ALLENE OXIDE CYCLASE (AOC) gene family members of Arabidopsis thaliana: tissue- and organ-specific promoter activities and in vivo heteromerization JO - J Exp Bot PY - 2012 SP - 6125-6138 AU - Stenzel, I. AU - Otto, M. AU - Delker, C. AU - Kirmse, N. AU - Schmidt, D. AU - Miersch, O. AU - Hause, B. AU - Wasternack, C. VL - 63 UR - https://dx.doi.org/10.1093/jxb/ers261 DO - 10.1093/jxb/ers261 AB - Jasmonates are important signals in plant stress responses and plant development. An essential step in the biosynthesis of jasmonic acid (JA) is catalysed by ALLENE OXIDE CYCLASE (AOC) which establishes the naturally occurring enantiomeric structure of jasmonates. In Arabidopsis thaliana, four genes encode four functional AOC polypeptides (AOC1, AOC2, AOC3, and AOC4) raising the question of functional redundancy or diversification. Analysis of transcript accumulation revealed an organ-specific expression pattern, whereas detailed inspection of transgenic lines expressing the GUS reporter gene under the control of individual AOC promoters showed partially redundant promoter activities during development: (i) In fully developed leaves, promoter activities of AOC1, AOC2, and AOC3 appeared throughout all leaf tissue, but AOC4 promoter activity was vascular bundle-specific; (ii) only AOC3 and AOC4 showed promoter activities in roots; and (iii) partially specific promoter activities were found for AOC1 and AOC4 in flower development. In situ hybridization of flower stalks confirmed the GUS activity data. Characterization of single and double AOC loss-of-function mutants further corroborates the hypothesis of functional redundancies among individual AOCs due to a lack of phenotypes indicative of JA deficiency (e.g. male sterility). To elucidate whether redundant AOC expression might contribute to regulation on AOC activity level, protein interaction studies using bimolecular fluorescence complementation (BiFC) were performed and showed that all AOCs can interact among each other. The data suggest a putative regulatory mechanism of temporal and spatial fine-tuning in JA formation by differential expression and via possible heteromerization of the four AOCs. KW - Cell Biology A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER - TY - JOUR ID - 1219 TI - De-regulation of abscisic acid contents causes abnormal endosperm development in the barley mutant seg8 JO - Plant J PY - 2010 SP - 589-603 AU - Sreenivasulu, N. AU - Radchuk, V. AU - Alawady, A. AU - Borisjuk, L. AU - Weier, D. AU - Staroske, N. AU - Fuchs, J. AU - Miersch, O. AU - Strickert, M. AU - Usadel, B. AU - Wobus, U. AU - Grimm, B. AU - Weber, H. AU - Weschke, W. VL - 64(4) UR - http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2010.04350.x/abstract DO - 10.1111/j.1365-313X.2010.04350.x AB - Grain development of the maternal effect shrunken endosperm mutant seg8 was analysed by comprehensive molecular, biochemical and histological methods. The most obvious finding was de-regulation of ABA levels, which were lower compared to wild-type during the pre-storage phase but higher during the transition from cell division/differentiation to accumulation of storage products. Ploidy levels and ABA amounts were inversely correlated in the developing endosperms of both mutant and wild-type, suggesting an influence of ABA on cell-cycle regulation. The low ABA levels found in seg8 grains between anthesis and beginning endosperm cellularization may result from a gene dosage effect in the syncytial endosperm that causes impaired transfer of ABA synthesized in vegetative tissues into filial grain parts. Increased ABA levels during the transition phase are accompanied by higher chlorophyll and carotenoid/xanthophyll contents. The data suggest a disturbed ABA-releasing biosynthetic pathway. This is indicated by up-regulation of expression of the geranylgeranyl reductase (GGR) gene, which may be induced by ABA deficiency during the pre-storage phase. Abnormal cellularization/differentiation of the developing seg8 endosperm and reduced accumulation of starch are phenotypic characteristics that reflect these disturbances. The present study did not reveal the primary gene defect causing the seg8 phenotype, but presents new insights into the maternal/filial relationships regulating barley endosperm development. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 1039 TI - PAMP-induced defense responses in potato require both salicylic acid and jasmonic acid JO - Plant Journal PY - 2009 SP - 230 - 242 AU - Halim, V.A. AU - Altmann, S. AU - Ellinger, D. AU - Eschen-Lippold, L. AU - Miersch, O. AU - Scheel, D. AU - Rosahl, S. VL - 57 UR - http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2008.03688.x/abstract DO - 10.1111/j.1365-313X.2008.03688.x AB - To elucidate the molecular mechanisms underlying pathogen-associated molecular pattern (PAMP)-induced defense responses in potato (Solanum tuberosum), the role of the signaling compounds salicylic acid (SA) and jasmonic acid (JA) was analyzed. Pep-13, a PAMP from Phytophthora, induces the accumulation of SA, JA and hydrogen peroxide, as well as the activation of defense genes and hypersensitive-like cell death. We have previously shown that SA is required for Pep-13-induced defense responses. To assess the importance of JA, RNA interference constructs targeted at the JA biosynthetic genes, allene oxide cyclase and 12- oxophytodienoic acid reductase, were expressed in transgenic potato plants. In addition, expression of the F-box protein COI1 was reduced by RNA interference. Plants expressing the RNA interference constructs failed to accumulate the respective transcripts in response to wounding or Pep-13 treatment, neither did they contain significant amounts of JA after elicitation. In response to infiltration of Pep-13, the transgenic plants exhibited a highly reduced accumulation of reactive oxygen species as well as reduced hypersensitive cell death. The ability of the JA-deficient plants to accumulate SA suggests that SA accumulation is independent or upstream of JA accumulation. These data show that PAMP responses in potato require both SA and JA and that, in contrast to Arabidopsis, these compounds act in the same signal transduction pathway. Despite their inability to fully respond to PAMP treatment, the transgenic RNA interference plants are not altered in their basal defense against Phytophthora infestans. A2 - C1 - Stress and Developmental Biology; Molecular Signal Processing ER - TY - JOUR ID - 1080 TI - (+)-7-iso-Jasmonoyl-L-isoleucine is the endogenous bioactive jasmonate JO - Nat Chem Biol PY - 2009 SP - 344-350 AU - Fonseca, S. AU - Chini, A. AU - Hamberg, M. AU - Adie, B. AU - Porzel, A. AU - Kramell, R. AU - Miersch, O. AU - Wasternack, C. AU - Solano, R. VL - 5 UR - http://www.nature.com/nchembio/journal/v5/n5/full/nchembio.161.html DO - 10.1038/nchembio.161 AB - Hormone-triggered activation of the jasmonate signaling pathway in Arabidopsis thaliana requires SCFCOI1-mediated proteasome degradation of JAZ repressors. (-)-JA-L-Ile is the proposed bioactive hormone, and SCFCOI1 is its likely receptor. We found that the biological activity of (-)-JA-L-Ile is unexpectedly low compared to coronatine and the synthetic isomer (+)-JA-L-Ile, which suggests that the stereochemical orientation of the cyclopentanone-ring side chains greatly affects receptor binding. Detailed GC-MS and HPLC analyses showed that the (-)-JA-L-Ile preparations currently used in ligand binding studies contain small amounts of the C7 epimer (+)-7-iso-JA-L-Ile. Purification of each of these molecules demonstrated that pure (-)-JA-L-Ile is inactive and that the active hormone is (+)-7-iso-JA-L-Ile, which is also structurally more similar to coronatine. In addition, we show that pH changes promote conversion of (+)-7-iso-JA-L-Ile to the inactive (-)-JA-L-Ile form, thus providing a simple mechanism that can regulate hormone activity through epimerization. A2 - C1 - Molecular Signal Processing; Bioorganic Chemistry ER - TY - CHAP ID - 1499 TI - Chronobiologische Phänomene und Jasmonatgehalt bei Viscum album L. T2 - Die Mistel in der Tumortherapie 2. Aktueller Stand der Forschung und klinische Anwendung PB - PY - 2009 SP - 49-56 AU - Dorka, R. AU - Miersch, O. AU - Hause, B. AU - Weik, P. AU - Wasternack, C. VL - UR - SN - 978-3-933351-82 AB - A2 - Scheer, R.; Bauer, R.; Bekker, A.; Berg, P. A.; Fintelmann, V. C1 - Molecular Signal Processing ER - TY - JOUR ID - 1043 TI - Jasmonates act with salicylic acid to confer basal thermotolerance in Arabidopsis thaliana JO - New Phytol PY - 2009 SP - 175-187 AU - Clarke, S.M. AU - Cristescu, S.M. AU - Miersch, O. AU - Harren, F.J.M. AU - Wasternack, C. AU - Mur, L.A.J. VL - 182 UR - http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2008.02735.x/abstract DO - 10.1111/j.1469-8137.2008.02735.x AB - The cpr5-1 Arabidopsis thaliana mutant exhibits constitutive activation of salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) signalling pathways and displays enhanced tolerance of heat stress (HS). cpr5-1 crossed with jar1-1 (a JA-amino acid synthetase) was compromised in basal thermotolerance, as were the mutants opr3 (mutated in OPDA reductase3) and coi1-1 (affected in an E3 ubiquitin ligase F-box; a key JA-signalling component). In addition, heating wild-type Arabidopsis led to the accumulation of a range of jasmonates: JA, 12-oxophytodienoic acid (OPDA) and a JA-isoleucine (JA-Ile) conjugate. Exogenous application of methyl jasmonate protected wild-type Arabidopsis from HS. Ethylene was rapidly produced during HS, with levels being modulated by both JA and SA. By contrast, the ethylene mutant ein2-1 conferred greater thermotolerance. These data suggest that JA acts with SA, conferring basal thermotolerance while ET may act to promote cell death. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 1042 TI - ADP-glucose pyrophosphorylase-deficient pea embryos reveal specific transcriptional and metabolic changes of carbon-nitrogen metabolism and stress responses JO - Plant Physiol PY - 2009 SP - 395-411 AU - Weigelt, K. AU - Küster, H. AU - Rutten, T. AU - Fait, A. AU - Fernie, A.R. AU - Miersch, O. AU - Wasternack, C. AU - Emery, R.J.N. AU - Desel, C. AU - Hosein, F. AU - Müller, M. AU - Saalbach, I. AU - Weber, H. VL - 149 UR - DO - 10.1104/pp.108.129940 AB - We present a comprehensive analysis of ADP-glucose pyrophosphorylase (AGP)-repressed pea (Pisum sativum) seeds using transcript and metabolite profiling to monitor the effects that reduced carbon flow into starch has on carbon-nitrogen metabolism and related pathways. Changed patterns of transcripts and metabolites suggest that AGP repression causes sugar accumulation and stimulates carbohydrate oxidation via glycolysis, tricarboxylic acid cycle, and mitochondrial respiration. Enhanced provision of precursors such as acetyl-coenzyme A and organic acids apparently support other pathways and activate amino acid and storage protein biosynthesis as well as pathways fed by cytosolic acetyl-coenzyme A, such as cysteine biosynthesis and fatty acid elongation/metabolism. As a consequence, the resulting higher nitrogen (N) demand depletes transient N storage pools, specifically asparagine and arginine, and leads to N limitation. Moreover, increased sugar accumulation appears to stimulate cytokinin-mediated cell proliferation pathways. In addition, the deregulation of starch biosynthesis resulted in indirect changes, such as increased mitochondrial metabolism and osmotic stress. The combined effect of these changes is an enhanced generation of reactive oxygen species coupled with an up-regulation of energy-dissipating, reactive oxygen species protection, and defense genes. Transcriptional activation of mitogen-activated protein kinase pathways and oxylipin synthesis indicates an additional activation of stress signaling pathways. AGP-repressed embryos contain higher levels of jasmonate derivatives; however, this increase is preferentially in nonactive forms. The results suggest that, although metabolic/osmotic alterations in iAGP pea seeds result in multiple stress responses, pea seeds have effective mechanisms to circumvent stress signaling under conditions in which excessive stress responses and/or cellular damage could prematurely initiate senescence or apoptosis. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 1087 TI - Spodoptera littoralis-Induced Lectin Expression in Tobacco JO - Plant Cell Physiol PY - 2009 SP - 1142-1155 AU - Vandenborre, G. AU - Miersch, O. AU - Hause, B. AU - Smagghe, G. AU - Wasternack, C. AU - Van Damme, E.J.M. VL - 50 UR - 10.1093/pcp/pcp065 DO - 10.1093/pcp/pcp065 AB - The induced defense response in plants towards herbivores is mainly regulated by jasmonates and leads to the accumulation of so-called jasmonate-induced proteins. Recently, a jasmonate (JA) inducible lectin called Nicotiana tabacum agglutinin or NICTABA was discovered in tobacco( N. tabacum cv Samsun) leaves. Tobacco plants also accumulate the lectin after insect attack by caterpillars. To study the functional role of NICTABA, the accumulation of the JA precursor 12-oxophytodienoic acid (OPDA), JA as well as different JA metabolites were analyzed in tobacco leaves after herbivory by larvae of the cotton leafworm ( Spodoptera littoralis ) and correlated with NICTABA accumulation. It was shown that OPDA, JA as well as its methyl ester can trigger NICTABA accumulation. However, hydroxylation of JA and its subsequent sulfation and glucosylation results in inactive compounds that have lost the capacity to induce NICTABA gene expression. The expression profi le of NICTABA after caterpillar feeding was recorded in local as well as in systemic leaves, and compared to the expression of several genes encodingdefense proteins, and genes encoding a tobacco systemin and the allene oxide cyclase, an enzyme in JA biosynthesis. Furthermore, the accumulation of NICTABA was quantified after S. littoralis herbivory and immunofl uorescence microscopy was used to study the localization of NICTABA in the tobacco leaf. A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER - TY - JOUR ID - 984 TI - The AOC promoter of tomato is regulated by developmental and environmental stimuli JO - Phytochemistry PY - 2008 SP - 1859-1869 AU - Stenzel, I. AU - Hause, B. AU - Proels, R. AU - Miersch, O. AU - Oka, M. AU - Roitsch, T. AU - Wasternack, C. VL - 69 UR - DO - 10.1016/j.phytochem.2008.03.007 AB - A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER - TY - JOUR ID - 983 TI - Reduced V-ATPase Activity in the trans-Golgi Network Causes Oxylipin-Dependent Hypocotyl Growth Inhibition in Arabidopsis JO - The Plant Cell PY - 2008 SP - 1088-1100 AU - Brüx, A. AU - Liu, T-Y. AU - Krebs, M. AU - Stierhof, Y.-D. AU - Lohmann, J.U. AU - Miersch, O. AU - Wasternack, C. AU - Schumacher, K. VL - 20 UR - DO - 10.1105/tpc.108.058362 AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 981 TI - A MYB Transcription Factor Regulates Very-Long-Chain Fatty Acid Biosynthesis for Activation of the Hypersensitive Cell Death Response in Arabidopsis JO - The Plant Cell PY - 2008 SP - 752-767 AU - Raffaele, S. AU - Vailleau, F. AU - Léger, A. AU - Joubès, J. AU - Miersch, O. AU - Huard, C. AU - Blée, E. AU - Mongrand, S. AU - Domergue, F. AU - Roby, D. VL - 20 UR - DO - 10.1105/tpc.107.054858 AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 980 TI - Jasmonates meet fatty acids: functional analysis of a new acyl-coenzyme A synthetase family from Arabidopsis thaliana JO - J. Exp. Bot. PY - 2008 SP - 403-419 AU - Kienow, L. AU - Schneider, K. AU - Bartsch, M. AU - Stuible, H.-P. AU - Weng, H. AU - Miersch, O. AU - Wasternack, C. AU - Kombrink, E. VL - 59 (2) UR - DO - 10.1093/jxb/erm325 AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 943 TI - Hydroxylated jasmonates are commonly occurring metabolites of jasmonic acid and contribute to a partial switch-off in jasmonate signaling JO - New Phytologist PY - 2008 SP - 114-127 AU - Miersch, O. AU - Neumerkel, J. AU - Dippe, M. AU - Stenzel, I. AU - Wasternack, C. VL - 177 UR - DO - 10.1111/j.1469-8137.2007.02252.x AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 942 TI - Chronobiological phenomena and seasonal changes in jasmonate levels during the course of the year and under constant conditions in mistletoe (Viscum album L.) JO - Phytomedicine PY - 2007 SP - 15 AU - Dorka, R. AU - Miersch, O. AU - Wasternack, C. AU - Weik, P. VL - 14 UR - DO - 10.1016/j.phymed.2007.07.014 AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 916 TI - Endogenous jasmonates in dry and imbibed sunflower seeds from plants grown at different soil moisture contents JO - Seed Sci. Res. PY - 2007 SP - 91-98 AU - Vigliocco, A. AU - Alemano, S. AU - Miersch, O. AU - Alvarez, D. AU - Abdala, G. VL - 17 UR - DO - 10.1017/S0960258507708371 AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 908 TI - Cold and water stresses produce changes in endogenous jasmonates in two populations of Pinus pinaster Ait JO - Plant Growth Regul. PY - 2007 SP - 111-116 AU - Pedranzani, H. AU - Sierro-de-Grado, R. AU - Vigliocco, A. AU - Miersch, O. AU - Abdala, G. VL - 52 UR - DO - 10.1007/s10725-007-9166-2 AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 891 TI - The Jasmonate-Induced Expression of the Nicotiana tabacum Leaf Lectin JO - Plant and Cell Physiol. PY - 2007 SP - 1207-1218 AU - Lannoo, N. AU - Vandenborre, G. AU - Miersch, O. AU - Smagghe, G. AU - Wasternack, C. AU - Peumans, W.J. AU - Van Damme, E.J.M. VL - 48 UR - DO - 10.1093/pcp/pcm090 AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 877 TI - Jasmonate biosynthesis in Arabidopsis thaliana requires peroxisomal β-oxidation enzymes – Additional proof by properties of pex6 and aim1 JO - Phytochemistry PY - 2007 SP - 1642-1650 AU - Delker, C. AU - Zolman, B.K. AU - Miersch, O. AU - Wasternack, C. VL - 68 UR - DO - 10.1016/j.phytochem.2007.04.024 AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 845 TI - Substrate specificity and products of side-reactions catalyzed by jasmonate:amino acid synthetase (JAR1) JO - FEBS Letters PY - 2007 SP - 815-820 AU - Guranowski, A. AU - Miersch, O. AU - Staswick, P.E. AU - Suza, W. AU - Wasternack, C. VL - 581 UR - DO - 10.1016/j.febslet.2007.01.049 AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 781 TI - Jasmonate Biosynthesis in Arabidopsis thaliana - Enzymes, Products, Regulation JO - Plant Biol. PY - 2006 SP - 297-306 AU - Delker, C. AU - Stenzel, I. AU - Hause, B. AU - Miersch, O. AU - Feussner, I. AU - Wasternack, C. VL - 8 UR - http://onlinelibrary.wiley.com/doi/10.1055/s-2006-923935/abstract DO - 10.1055/s-2006-923935 AB - Among the plant hormones jasmonic acid and related derivatives are known to mediate stress responses and several developmental processes. Biosynthesis, regulation, and metabolism of jasmonic acid in Arabidopsis thaliana are reviewed, including properties of mutants of jasmonate biosynthesis. The individual signalling properties of several jasmonates are described. A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER - TY - JOUR ID - 701 TI - The wound response in tomato - Role of jasmonic acid JO - J. Plant Physiol PY - 2006 SP - 297-306 AU - Wasternack, C. AU - Stenzel, I. AU - Hause, B. AU - Hause, G. AU - Kutter, C. AU - Maucher, H. AU - Neumerkel, J. AU - Feussner, I. AU - Miersch, O. VL - 163 UR - DO - 10.1016/j.jplph.2005.10.014 AB - A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER - TY - JOUR ID - 794 TI - Comparative transcript and alkaloid profiling in Papaver species identifies a short chain dehydrogenase/reductase involved in morphine biosynthesis JO - Plant J PY - 2006 SP - 177-192 AU - Ziegler, J. AU - Voigtländer, S. AU - Schmidt, J. AU - Kramell, R. AU - Miersch, O. AU - Ammer, C. AU - Gesell, A. AU - Kutchan, T.M. VL - 48 UR - http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2006.02860.x/full DO - 10.1111/j.1365-313X.2006.02860.x AB - Plants of the order Ranunculales, especially members of the species Papaver, accumulate a large variety of benzylisoquinoline alkaloids with about 2500 structures, but only the opium poppy (Papaver somniferum) and Papaver setigerum are able to produce the analgesic and narcotic morphine and the antitussive codeine. In this study, we investigated the molecular basis for this exceptional biosynthetic capability by comparison of alkaloid profiles with gene expression profiles between 16 different Papaver species. Out of 2000 expressed sequence tags obtained from P. somniferum, 69 show increased expression in morphinan alkaloid-containing species. One of these cDNAs, exhibiting an expression pattern very similar to previously isolated cDNAs coding for enzymes in benzylisoquinoline biosynthesis, showed the highest amino acid identity to reductases in menthol biosynthesis. After overexpression, the protein encoded by this cDNA reduced the keto group of salutaridine yielding salutaridinol, an intermediate in morphine biosynthesis. The stereoisomer 7-epi-salutaridinol was not formed. Based on its similarities to a previously purified protein from P. somniferum with respect to the high substrate specificity, molecular mass and kinetic data, the recombinant protein was identified as salutaridine reductase (SalR; EC Unlike codeinone reductase, an enzyme acting later in the pathway that catalyses the reduction of a keto group and which belongs to the family of the aldo-keto reductases, the cDNA identified in this study as SalR belongs to the family of short chain dehydrogenases/reductases and is related to reductases in monoterpene metabolism. A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER - TY - JOUR ID - 792 TI - Interaction between nitric oxide and ethylene in the induction of alternative oxidase in ozone-treated tobacco plants JO - Plant Physiol. PY - 2006 SP - 595-608 AU - Ederli, L. AU - Morettini, R. AU - Borgogni, A. AU - Wasternack, C. AU - Miersch, O. AU - Reale, L. AU - Ferranti, F. AU - Tosit, N. AU - Pasqualini, S. VL - 142 UR - DO - 10.1104/pp.106.085472 AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 786 TI - Gene expression patterns reveal tissue-specific signaling networks controlling programmed cell death and ABA-regulated maturation in developing barley seeds JO - The Plant J. PY - 2006 SP - 310-327 AU - Sreenivasulu, N. AU - Radchuk, V. AU - Strickert, M. AU - Miersch, O. AU - Weschke, W. AU - Wobus, U. VL - 47 UR - DO - 10.1111/j.1365-313X.2006.02789.x AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 696 TI - The outcomes of concentration specific interactions between salicylate and jasmonate signaling include synergy, antagonism and the activation of cell death JO - Plant Physiol. PY - 2006 SP - 249-262 AU - Mur, L.A.J. AU - Kenton, P. AU - Atzorn, R. AU - Miersch, O. AU - Wasternack, C. VL - 140 UR - DO - 10.1104/pp.105.072348 AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 690 TI - Simultaneous determination of multiple phytohormones in plant extracts by liquid chromatography-electrospray tandem mass spectrometry JO - J. Agric. Food Chem. PY - 2005 SP - 8437-8442 AU - Durgbanshi, A. AU - Arbona, V. AU - Pozo, O. AU - Miersch, O. AU - Sancho, J.V. AU - Gómez-Cadenas, A. VL - 53 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 787 TI - Octadecanoid levels during stolon to tuber transition in potato. JO - Potato Res. PY - 2005 SP - 107-115 AU - Denzano, A.M. AU - Vigliocco, A. AU - Miersch, O. AU - Abdala, G. VL - UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 693 TI - Lack of mycorrhizal autoregulation and phytohormonal changes in the supernodulating soybean mutant nts1007 JO - Planta PY - 2005 SP - 709-715 AU - Meixner, C. AU - Ludwig-Müller, J. AU - Miersch, O. AU - Gresshoff, P. AU - Staehlin, C. AU - Vierheilig, H. VL - 222 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 433 TI - A new type of peroxisomal acyl-coenzyme A synthetase from Arabidopsis thaliana has the catalytic capacity of activate biosynthetic precursors of jasmonic acid JO - J. Biol. Chem. PY - 2005 SP - 13962-13972 AU - Schneider, K. AU - Kienow, L. AU - Schmelzer, E. AU - Colby, T. AU - Bartsch, M. AU - Miersch, O. AU - Wasternack, C. AU - Kombrink, E. AU - Stuible, H.-P. VL - 280 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 689 TI - Endogenous jasmonates and octadecanoids during germination and seedling development: their relation with hypersensitive tomato mutants to abiotic stress JO - Seed Sci. Res. PY - 2005 SP - 309-318 AU - Andrade, A. AU - Vigliocco, A. AU - Alemano, S. AU - Miersch, O. AU - Botella, M.A. VL - 15 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 691 TI - Expression of allene oxide cyclase and accumulation of jasmonates during organogenic nodule formation from hop (Humulus lupulus var. Nugget) internodes JO - Plant Cell Physiol. PY - 2005 SP - 1713-23 AU - Fortes, A.M. AU - Miersch, O. AU - Lange, P.R. AU - Malho, R. AU - Testillano, P.S. AU - del Risueno, M.C. AU - Wasternack, C. AU - Pais, M.S. VL - 46 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 435 TI - Concurrent activation of cell death-regulating signaling pathways by singlet oxygen in Arabidopsis thaliana JO - Plant J. PY - 2005 SP - 68-80 AU - Danon, A. AU - Miersch, O. AU - Felix, G. AU - op den Camp, R.G.L. AU - Apel, K. VL - 41 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 694 TI - Changes in jasmonates and 12-oxophytodienoic acid contents of Medicago sativa L. during somatic embryogenesis JO - Acta Physiol. Plantar. PY - 2005 SP - 497-504 AU - Rudus, I. AU - Kepczynska, E. AU - Kepczynski, J. AU - Wasternack, C. AU - Miersch, O. VL - 27 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 542 TI - Ethylene-mediated cross-talk between calcium-dependent protein kinase and MAPK signaling controls stress responses in plants JO - PNAS PY - 2005 SP - 10736-10741 AU - Ludwig, A.A. AU - Saitoh, H. AU - Felix, G. AU - Freymark, G. AU - Miersch, O. AU - Wasternack, C. AU - Boller, T. AU - Jones, J.D.G. AU - Romeis, T. VL - 102 UR - AB - A2 - C1 - Molecular Signal Processing; Biochemistry of Plant Interactions ER - TY - JOUR ID - 371 TI - The allene oxide cyclase of barley (Hordeum vulgare L.) - cloning and organ-specific expression JO - Phytochemistry PY - 2004 SP - 801-811 AU - Maucher, H. AU - Stenzel, I. AU - Miersch, O. AU - Stein, N. AU - Prasad, M. AU - Zierold, U. AU - Schweizer, P. AU - Dorer, C. AU - Hause, B. AU - Wasternack, C. VL - 65 UR - DO - 10.1016/j.phytochem.2004.01.009 AB - A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER - TY - JOUR ID - 360 TI - Biochemical and molecular characterization of a hydroxy-jasmonate sulfotransferase from Arabidopsis thaliana JO - J. Biol. Chem. PY - 2003 SP - 17895-17900 AU - Gidda, K.S. AU - Miersch, O. AU - Schmidt, J. AU - Wasternack, C. AU - Varin, L. VL - 278 UR - http://www.jbc.org/content/by/year DO - 10.1074/jbc.M211943200 AB - 12-Hydroxyjasmonate, also known as tuberonic acid, was first isolated from Solanum tuberosum and was shown to have tuber-inducing properties. It is derived from the ubiquitously occurring jasmonic acid, an important signaling molecule mediating diverse developmental processes and plant defense responses. We report here that the gene AtST2a from Arabidopsis thaliana encodes a hydroxyjasmonate sulfotransferase. The recombinant AtST2a protein was found to exhibit strict specificity for 11- and 12-hydroxyjasmonate with Km values of 50 and 10 µM, respectively. Furthermore, 12-hydroxyjasmonate and its sulfonated derivative are shown to be naturally occurring in A. thaliana. The exogenous application of methyljasmonate to A. thaliana plants led to increased levels of both metabolites, whereas treatment with 12-hydroxyjasmonate led to increased level of 12-hydroxyjasmonate sulfate without affecting the endogenous level of jasmonic acid. AtST2a expression was found to be induced following treatment with methyljasmonate and 12-hydroxyjasmonate. In contrast, the expression of the methyljasmonate-responsive gene Thi2.1, a marker gene in plant defense responses, is not induced upon treatment with 12-hydroxyjasmonate indicating the existence of independent signaling pathways responding to jasmonic acid and 12-hydroxyjasmonic acid. Taken together, the results suggest that the hydroxylation and sulfonation reactions might be components of a pathway that inactivates excess jasmonic acid in plants. Alternatively, the function of AtST2a might be to control the biological activity of 12-hydroxyjasmonic acid. A2 - C1 - Molecular Signal Processing; Bioorganic Chemistry ER - TY - JOUR ID - 359 TI - Selective desensitization of jasmonate- and pH-dependent signaling in the induction of benzophenanthridine biosynthesis in cells of Eschscholzia californica JO - Phytochemistry PY - 2003 SP - 491-500 AU - Färber, K. AU - Schumann, B. AU - Miersch, O. AU - Roos, W. VL - 62 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 367 TI - Multiple hormones cooperatively control a susceptible tomato pathogen defense response JO - Plant Physiol. PY - 2003 SP - 1181-1189 AU - O'Donnell, P.J. AU - Schmelz, E. AU - Block, A. AU - Miersch, O. AU - Wasternack, C. AU - Jones, J.B. AU - Klee, H.J. VL - 133 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 358 TI - Jasmonate and octadecanoid occurrence in tomato hairy roots. Endogenous level changes in response to NaCl JO - Plant Growth Regul. PY - 2003 SP - 21-27 AU - Abdala, G. AU - Miersch, O. AU - Kramell, R. AU - Vigliocco, A. AU - Agostini, E. AU - Forchetti, G. AU - Alemano, S. VL - 40 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 452 TI - Induction of jasmonate biosynthesis in arbuscular mycorrhizal barley roots JO - Plant Physiol. PY - 2002 SP - 1213-1220 AU - Hause, B. AU - Maier, W. AU - Miersch, O. AU - Kramell, R. AU - Strack, D. VL - 130 UR - http://www.ncbi.nlm.nih.gov/pmc/journals/69/ DO - 10.1104/pp.006007 AB - Colonization of barley (Hordeum vulgare cv Salome) roots by an arbuscular mycorrhizal fungus, Glomus intraradices Schenck & Smith, leads to elevated levels of endogenous jasmonic acid (JA) and its amino acid conjugate JA-isoleucine, whereas the level of the JA precursor, oxophytodienoic acid, remains constant. The rise in jasmonates is accompanied by the expression of genes coding for an enzyme of JA biosynthesis (allene oxide synthase) and of a jasmonate-induced protein (JIP23). In situ hybridization and immunocytochemical analysis revealed that expression of these genes occurred cell specifically within arbuscule-containing root cortex cells. The concomitant gene expression indicates that jasmonates are generated and act within arbuscule-containing cells. By use of a near-synchronous mycorrhization, analysis of temporal expression patterns showed the occurrence of transcript accumulation 4 to 6 d after the appearance of the first arbuscules. This suggests that the endogenous rise in jasmonates might be related to the fully established symbiosis rather than to the recognition of interacting partners or to the onset of interaction. Because the plant supplies the fungus with carbohydrates, a model is proposed in which the induction of JA biosynthesis in colonized roots is linked to the stronger sink function of mycorrhizal roots compared with nonmycorrhizal roots. A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER - TY - JOUR ID - 355 TI - Activation of jasmonic acid production in Zea mays L. infected by the maize rough dwarf virus-Río Cuarto. Reversion of symptoms by salicylic acid JO - Biocell PY - 2002 SP - 369-374 AU - Vigliocco, A. AU - Bonamico, M.B. AU - Alemano, S. AU - Miersch, O. AU - Abdala, G. VL - 26(3) UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 352 TI - Cell death and salicylate- and jasmonate-dependent stress responses in Arabidopsis are controlled by single cet genes JO - Planta PY - 2002 SP - 120-128 AU - Nibbe, M. AU - Hilpert, B. AU - Wasternack, C. AU - Miersch, O. AU - Apel, K. VL - 216 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 347 TI - Changes in jasmonate and gibberellin levels during development of potato plants (Solanum tuberosum) JO - Plant Growth Reg. PY - 2002 SP - 121-126 AU - Abdala, G. AU - Castro, G. AU - Miersch, O. AU - Pierce, D. VL - 36 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 344 TI - Isolation and characterization of signal transduction mutants of Arabidopsis thaliana that constitutively activate the octadecanoid pathway and form necrotic microlesions JO - Plant J. PY - 2001 SP - 435-446 AU - Hilpert, B. AU - Bohlmann, H. AU - Den Camp, R.O. AU - Przybyla, D. AU - Miersch, O. AU - Buchala, A. AU - Apel, K. VL - 26 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 97 TI - Molecular cloning of allene oxide cyclase: The enzyme establishing the stereochemistry of octadecanoids and jasmonates JO - J. Biol. Chem. PY - 2000 SP - 19132-19138 AU - Ziegler, J. AU - Stenzel, I. AU - Hause, B. AU - Maucher, H. AU - Miersch, O. AU - Hamberg, M. AU - Grimm, M. AU - Ganal, M. AU - Wasternack, C. VL - 275 UR - http://www.jbc.org/content/275/25/19132.abstract?sid=04f09be3-5f6e-4d78-aa97-a7b681940e00 DO - 10.1074/jbc.M002133200 AB - Allene oxide cyclase (AOC) catalyses the stereospecific cyclisation of an unstable allene oxide to 9(S),13(S)-12-oxo-10,15(Z)-phytodienoic acid, the ultimate precursor of jasmonic acid. This enzyme has previously been purified, and two identical N-terminal peptides were found suggesting AOC to be a homodimeric protein. Furthermore, the native protein was N-terminal processed. Using degenerate primers, a PCR fragment could be generated from tomato, which was further used to isolate a full length cDNA clone of 1kb coding for a protein with 245 amino acids with a molecular mass of 26 kDa. Whereas expression of the whole coding region failed to detect AOC activity, a 5-'truncated protein showed high activity, suggesting that additional amino acids impair the enzymatic function. Steric analysis of the 12-oxo-phytodienoic acid formed by the recombinant AOC revealed exclusive (>99%) formation of the 9(S),13(S) enantiomer. Exclusive formation of this enantiomer was also found in wounded tomato leaves. Southern analysis and genetic mapping revealed the existence of a single gene for AOC located on chromosome 2 of tomato. Inspection of the N-terminus revealed the presence of a chloroplastic transit peptide, and the location of AOC protein in that compartment could be shown by immunohistochemical methods. Concomitant with the jasmonate levels, the accumulation of AOC mRNA was transiently induced after wounding of tomato leaves. A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER - TY - JOUR ID - 339 TI - Octadecanoid and jasmonate signaling in tomato leaves (Lycopersicon esculentum Mill.): Endogenous jasmonates do not induce jasmonate biosynthesis JO - Biol. Chem. PY - 2000 SP - 715-722 AU - Miersch, O. AU - Wasternack, C. VL - 381 UR - AB - Jasmonates and their precursors, the octadecanoids, are signals in stress-induced alteration of gene expression. Several mRNAs coding for enzymes of jasmonic acid (JA) biosynthesis are up-regulated upon JA treatment or endogenous rise of JA level. Here we inspected the positive feed back of endogenous JA on JA formation as well as its beta-oxidation steps. JA responsive gene expression was recorded in terms of proteinase inhibitor2 (pin2) mRNA accumulation. JA formed upon treatment of tomato (Lycopersicon esculentum cv. Moneymaker) leaves with JA derivatives carrying different length of the carboxylic acid side chain was quantified by gas chromatography-mass spectrometry (GC-MS). The data reveal that beta-oxidation of the side chain occurs up to a butyric acid moiety. The amount of JA formed from side-chain modified JA derivatives, correlated with pin2-mRNA accumulation. JA derivatives with a carboxylic side chain of 3, 5 or 7 carbon atoms were unable to form JA and to express on pin2, whereas even numbered derivatives were active. After treatment of tomato leaves with (10-2H)-(-)-12-oxophytoenoic acid, (4-2H)-(-)-JA and its methyl ester were formed and could be quantified separately from the endogenously unlabeled JA pool by GC-MS analysis via isotopic discrimination. The level of 8 nmol per g f.w. JA and its methyl ester originated exclusively from labeled 12-oxophytoenic acid. This and further data indicate that endogenous synthesis of the JA precursor 12-oxophytodienoic acid as well as of JA and its methyl ester are not induced in tomato leaves, suggesting that positive feedback in JA biosynthesis does not function in vivo. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 337 TI - Octadecanoid-derived alteration of gene expression and the 'oxylipin signature' in stressed barley leaves - implications for different signalling pathways JO - Plant Physiol. PY - 2000 SP - 177-186 AU - Kramell, R. AU - Miersch, O. AU - Atzorn, R. AU - Parthier, B. AU - Wasternack, C. VL - 123 UR - AB - Stress-induced gene expression in barley (Hordeum vulgare cv. Salome) leaves has been correlated with temporally changing levels of octadecanoids and jasmonates, quantified by means of gas chromatography/mass spectrometry-single ion monitoring. Application of sorbitol-induced stress led to a low and transient rise of jasmonic acid (JA), its precursor 12-oxophytodienoic acid (OPDA) and the methyl esters JAME and OPDAME, respectively, followed by a large increase in their levels. JA and JAME peaked between 12 and 16 h, about 4 hours before OPDA and OPDAME. However, OPDA accumulated up to a 2.5-fold higher level than the other compounds. Dihomo-jasmonic acid and 9,13-didehydro-12- oxophytoenoic acid were identified as minor components. Kinetic analyses revealed that a transient threshold of jasmonates or octadecanoids is necessary and sufficient to initiate JA responsive gene expression. Although OPDA and OPDAME applied exogenously were metabolized to JA in considerable amounts, both of them can induce gene expression per se as evidenced by those genes which do not respond to endogenously formed JA. Also, coronatine induces JA-responsive genes independently from endogenous JA. As evidenced by application of deuterated JA, endogenous synthesis of JA is not induced by JA treatment. The data are discussed in terms of distinct signalling pathways. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 421 TI - Liquid chromatography of jasmonic acid amine conjugates JO - Chromatographia PY - 1999 SP - 42-46 AU - Kramell, R. AU - Miersch, O. AU - Schneider, G. AU - Wasternack, C. VL - 49 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 422 TI - Jasmonates and related compounds form Fusarium oxysporum JO - Phytochemistry PY - 1999 SP - 517-523 AU - Miersch, O. AU - Bohlmann, H. AU - Wasternack, C. VL - 50 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 331 TI - Structure-activity relations of substituted, deleted or stereospecifically altered jasmonic acid in gene expression of barley leaves JO - Phytochemistry PY - 1999 SP - 353-361 AU - Miersch, O. AU - Kramell, R. AU - Parthier, B. AU - Wasternack, C. VL - 50 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 407 TI - A jasmonic acid conjugate, N-[()-jasmonoyl]-tyramine, from Petunia pollen JO - Phytochemistry PY - 1998 SP - 327-329 AU - Miersch, O. AU - Knöfel, H.-D. AU - Schmidt, J. AU - Kramell, R. AU - Parthier, B. VL - 47 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - CHAP ID - 417 TI - Characterization of isoleucine conjugates of cucurbic acid isomers by reversed-phase and chiral high-performance liquid chromatography T2 - Natural Product Analysis PB - P. Vieweg, Wiesbaden PY - 1998 SP - 77 AU - Kramell, R. AU - Porzel, A. AU - Miersch, O. AU - Schneider, G. VL - 0 UR - AB - A2 - Schreier, P., Herderich, M., Humpf, H.-U., Schwab, W. C1 - Molecular Signal Processing ER - TY - JOUR ID - 404 TI - Wounding and chemicals induce expression of the Arabidopsis gene Thi2.1, encoding a fungal defense thionin, via the octadecanoid pathway JO - FEBS Letters PY - 1998 SP - 281-286 AU - Bohlmann, H. AU - Vignutelli, A. AU - Hilpert, B. AU - Miersch, O. AU - Wasternack, C. AU - Apel, K. VL - 437 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 522 TI - Concentration of dilute protein solutions prior to sodium dodecylsulfate polyacrylamide gel electrophoresis JO - Anal. Biochem PY - 1997 SP - 257-260 AU - Ziegler, J. AU - Vogt, T. AU - Miersch, O. AU - Strack, D. VL - 250 UR - http://www.sciencedirect.com/science/article/pii/S000326979792248X DO - 10.1006/abio.1997.2248 AB - A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER - TY - JOUR ID - 396 TI - Partial purification and characterization of a jasmonic acid conjugate cleaving amidohydrolase from the fungus Botryodiplodia theobromae JO - FEBS Letters PY - 1997 SP - 105-110 AU - Hertel, S. AU - Knöfel, H.-D. AU - Kramell, R. AU - Miersch, O. VL - 407 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 400 TI - Purification and characterization of allene oxide cyclase from dry corn seeds JO - Plant Physiol. PY - 1997 SP - 565-573 AU - Ziegler, J. AU - Hamberg, M. AU - Miersch, O. AU - Parthier, B. VL - 114 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - CHAP ID - 401 TI - Allene oxide cyclase from corn: Partial purification and characterization T2 - Physiology, Biochemistry and Molecular Biology of Plant Lipids PB - Kluwer Academic Publishers, Dordrecht PY - 1997 SP - 99-101 AU - Ziegler, J. AU - Hamberg, M. AU - Miersch, O. VL - 0 UR - AB - A2 - Williams, J.P., Mobashsher, U., Khan, M.U., Lem, N.W. C1 - ER - TY - JOUR ID - 397 TI - Chiral separation of amide conjugates of jasmonic acid by liquid chromatography JO - Chromatographia PY - 1997 SP - 104-108 AU - Kramell, R. AU - Schneider, G. AU - Miersch, O. VL - 45 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 379 TI - Occurrence of jasmonic acid in organs of Solanum tuberosum L. c.v. Spunta and its effect on tuberization JO - Plant Growth Reg. PY - 1996 SP - 139-143 AU - Abdala, G. AU - Castro, G. AU - Guinazu, M. AU - Tizio, R. AU - Miersch, O. VL - 19 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 376 TI - Occurrence and identification of jasmonic acid and its amino acid conjugates induced by osmotic stress in barley leaf tissue JO - J. Plant Growth Reg. PY - 1995 SP - 29-36 AU - Kramell, R. AU - Atzorn, R. AU - Schneider, G. AU - Miersch, O. AU - Brückner, C. AU - Schmidt, J. AU - Sembdner, G. AU - Parthier, B. VL - 14 UR - AB - A2 - C1 - Molecular Signal Processing ER -