Binarová, P.; Hause, B.; Dolezel, J.; Dráber, P. Association of g-tubulin with kinetochore/centromeric region of plant chromosomes Plant J. 14, 751-757, (1998) DOI: 10.1046/j.1365-313x.1998.00166.x
Monoclonal antibodies raised against a phylogenetically conserved peptide from the C-terminal domain of g-tubulin molecule were used for immunofluorescence detection of g-tubulin in acentriolar mitotic spindles of plant cells. The antibodies stained kinetochore fibres along their whole length, including the close vicinity of kinetochores. After microtubule disassembly by the anti-microtubular drugs aminoprophos-methyl (APM), oryzalin, and colchicine, g -tubulin was found on remnants of kinetochore fibres attached to chromosomes. In cells recovering from the amiprophosmethyl treatment, g-tubulin was localized with the re-growing kinetochore microtubule fibres nucleated or captured by kinetochore/centromeric regions. On isolated chromosomes, g-tubulin co-localized with g-tubulin in the kinetochore/centromeric region. The presented data suggest that in acentriolar higher plant cells g -tubulin might be directly or indirectly involved in modulation and/or stabilization of kinetochore-microtubule interactions.