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Publikationen - Molekulare Signalverarbeitung

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Publikation

Hause, B.; Vörös, K.; Kogel, K.-H.; Besser, K.; Wasternack, C.; A Jasmonate-responsive Lipoxygenase of Barley Leaves is Induced by Plant Activators but not by Pathogens J. Plant Physiol. 154, 459-462, (1999) DOI: 10.1016/S0176-1617(99)80283-1

Using the recently isolated eDNA clone LOX2 : Hv : 1 which codes for the most abundant jasmonateinducible lipoxygenase (LOX) in barley leaves (Vörös et al., 1998), we analysed the capability of different activators of systemic activated resistance (SAR) to induce the expression of that LOX. Upon treatment of barley leaves with salicylate, 2,6-dichloroisonicotinic acid and benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester, all these compounds were able to induce the expression of the LOX2 : Hv : 1 gene, whereas upon infection with the powdery mildew fungus (Blumeria graminis f. sp. hordei) mRNA accumulation was not detectable in compatible or in incompatible interactions. The induction of the LOX2 : Hv : 1 protein by SAR activators and the expression of different sets of genes induced by jasmonate and salicylate, respectively, are discussed in relation to defense responses against pathogenic fungi.
Publikation

Kramell, R.; Miersch, O.; Schneider, G.; Wasternack, C.; Liquid chromatography of jasmonic acid amine conjugates Chromatographia 49, 42-46, (1999) DOI: 10.1007/BF02467185

Racemic jasmonic acid (3R,7R/3S,7S)-(±)-JA) was chemically conjugated with different biogenic amines originating from aliphatic and aromatic α-amino acids by decarboxylation. The resulting isomeric compounds were subjected to reversed-phase high-performance liquid chromatography (HPLC) and to HPLC on the chiral stationary phases Chiralpak AS and Nucleodex β-PM. Under reversed-phase conditions, all the homologous amine derivatives tested could be separated from each other except the JA-conjugates containing 2-phenyl-ethylamine and 3-methylbutylamine. On both chiral supports the (3R,7R)-(−)-JA conjugates eluted earlier than those of the enantiomeric counterpart (3S,7S)-(+)-JA. On Chiralpak AS all the isomers studied could be separated to baseline with a mobile phase containingn-hexane and 2-propanol. The calculated resolution factors were between 1.80 and 4.17. The pairs of isomers were also chromatographed on the cyclodextrin stationary phase Nucleodex β-PM with methanol-triethylammonium acetate buffer as mobile phase. Under these conditions resolution factors were between 0.74 and 1.29. The individual isomers were chiroptically characterized by measurement of their circular dichroism.
Publikation

Jablonická, V.; Ziegler, J.; Vatehová, Z.; Lišková, D.; Heilmann, I.; Obložinský, M.; Heilmann, M.; Inhibition of phospholipases influences the metabolism of wound-induced benzylisoquinoline alkaloids in Papaver somniferum L. J. Plant Physiol. 223, 1-8, (2018) DOI: 10.1016/j.jplph.2018.01.007

Benzylisoquinoline alkaloids (BIAs) are important secondary plant metabolites and include medicinally relevant drugs, such as morphine or codeine. As the de novo synthesis of BIA backbones is (still) unfeasible, to date the opium poppy plant Papaver somniferum L. represents the main source of BIAs. The formation of BIAs is induced in poppy plants by stress conditions, such as wounding or salt treatment; however, the details about regulatory processes controlling BIA formation in opium poppy are not well studied. Environmental stresses, such as wounding or salinization, are transduced in plants by phospholipid-based signaling pathways, which involve different classes of phospholipases. Here we investigate whether pharmacological inhibition of phospholipase A2 (PLA2, inhibited by aristolochic acid (AA)) or phospholipase D (PLD; inhibited by 5-fluoro-2-indolyl des-chlorohalopemide (FIPI)) in poppy plants influences wound-induced BIA accumulation and the expression of key biosynthetic genes. We show that inhibition of PLA2 results in increased morphinan biosynthesis concomitant with reduced production of BIAs of the papaverine branch, whereas inhibition of PLD results in increased production of BIAs of the noscapine branch. The data suggest that phospholipid-dependent signaling pathways contribute to the activation of morphine biosynthesis at the expense of the production of other BIAs in poppy plants. A better understanding of the effectors and the principles of regulation of alkaloid biosynthesis might be the basis for the future genetic modification of opium poppy to optimize BIA production.
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