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Publikation

Renovell, Á.; Gago, S.; Ruiz-Ruiz, S.; Velázquez, K.; Navarro, L.; Moreno, P.; Vives, M. C.; Guerri, J. Mapping the subgenomic RNA promoter of the Citrus leaf blotch virus coat protein gene by Agrobacterium-mediated inoculation Virology 406, 360-369, (2010) DOI: 10.1016/j.virol.2010.07.034

Citrus leaf blotch virus has a single-stranded positive-sense genomic RNA (gRNA) of 8747 nt organized in three open reading frames (ORFs). The ORF1, encoding a polyprotein involved in replication, is translated directly from the gRNA, whereas ORFs encoding the movement (MP) and coat (CP) proteins are expressed via 3' coterminal subgenomic RNAs (sgRNAs). We characterized the minimal promoter region critical for the CP-sgRNA expression in infected cells by deletion analyses using Agrobacterium-mediated infection of Nicotiana benthamiana plants. The minimal CP-sgRNA promoter was mapped between nucleotides −67 and + 50 nt around the transcription start site. Surprisingly, larger deletions in the region between the CP-sgRNA transcription start site and the CP translation initiation codon resulted in increased CP-sgRNA accumulation, suggesting that this sequence could modulate the CP-sgRNA transcription. Site-specific mutational analysis of the transcription start site revealed that the + 1 guanylate and the + 2 adenylate are important for CP-sgRNA synthesis.
Publikation

Vandenborre, G.; Miersch, O.; Hause, B.; Smagghe, G.; Wasternack, C.; Van Damme, E.J.M. Spodoptera littoralis-Induced Lectin Expression in Tobacco Plant Cell Physiol 50, 1142-1155, (2009) DOI: 10.1093/pcp/pcp065

The induced defense response in plants towards herbivores is mainly regulated by jasmonates and leads to the accumulation of so-called jasmonate-induced proteins. Recently, a jasmonate (JA) inducible lectin called Nicotiana tabacum agglutinin or NICTABA was discovered in tobacco( N. tabacum cv Samsun) leaves. Tobacco plants also accumulate the lectin after insect attack by caterpillars. To study the functional role of NICTABA, the accumulation of the JA precursor 12-oxophytodienoic acid (OPDA), JA as well as different JA metabolites were analyzed in tobacco leaves after herbivory by larvae of the cotton leafworm ( Spodoptera littoralis ) and correlated with NICTABA accumulation. It was shown that OPDA, JA as well as its methyl ester can trigger NICTABA accumulation. However, hydroxylation of JA and its subsequent sulfation and glucosylation results in inactive compounds that have lost the capacity to induce NICTABA gene expression. The expression profi le of NICTABA after caterpillar feeding was recorded in local as well as in systemic leaves, and compared to the expression of several genes encodingdefense proteins, and genes encoding a tobacco systemin and the allene oxide cyclase, an enzyme in JA biosynthesis. Furthermore, the accumulation of NICTABA was quantified after S. littoralis herbivory and immunofl uorescence microscopy was used to study the localization of NICTABA in the tobacco leaf.
Publikation

Carbonell, A.; Martínez de Alba, A.E.; Flores, R.; Gago, S. Double-stranded RNA interferes in a sequence-specific manner with the infection of representative members of the two viroid families Virology 371, 44-53, (2008) DOI: 10.1016/j.virol.2007.09.031

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Publikation

Fortes, A.M.; Miersch, O.; Lange, P.R.; Malho, R.; Testillano, P.S.; del Risueno, M.C.; Wasternack, C.; Pais, M.S. Expression of allene oxide cyclase and accumulation of jasmonates during organogenic nodule formation from hop (<EM>Humulus lupulus</EM> var. Nugget) internodes Plant Cell Physiol. 46, 1713-23, (2005)

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Publikation

Schüler, G.; Mithöfer, A.; Baldwin, I.T.; Berger, S.; Ebel, S.; Santos, J.G.; Herrmann, G.; Hölscher, D.; Kramell, R.; Kutchan, T.M.; Maucher, H.; Schneider, B.; Stenzel, I.; Wasternack, C.; Boland, W. Coronalon: a powerful tool in plant stress physiology FEBS Lett. 563, 17-22, (2004) DOI: 10.1016/S0014-5793(04)00239-X

Coronalon, a synthetic 6-ethyl indanoyl isoleucine conjugate, has been designed as a highly active mimic of octadecanoid phytohormones that are involved in insect and disease resistance. The spectrum of biological activities that is affected by coronalon was investigated in nine different plant systems specifically responding to jasmonates and/or 12-oxo-phytodienoic acid. In all bioassays analyzed, coronalon demonstrated a general strong activity at low micromolar concentrations. The results obtained showed the induction of (i) defense-related secondary metabolite accumulation in both cell cultures and plant tissues, (ii) specific abiotic and biotic stress-related gene expression, and (iii) root growth retardation. The general activity of coronalon in the induction of plant stress responses together with its simple and efficient synthesis suggests that this compound might serve as a valuable tool in the examination of various aspects in plant stress physiology. Moreover, coronalon might become employed in agriculture to elicit plant resistance against various aggressors.
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