@Article{IPB-2427, author = {Schulze, A. and Zimmer, M. and Mielke, S. and Stellmach, H. and Melnyk, C. W. and Hause, B. and Gasperini, D.}, title = {{Wound-Induced Shoot-to-Root Relocation of JA-Ile Precursors Coordinates Arabidopsis Growth}}, year = {2019}, pages = {1383-1394}, journal = {Mol Plant}, doi = {10.1016/j.molp.2019.05.013}, url = {https://dx.doi.org/10.1016/j.molp.2019.05.013}, volume = {12}, abstract = {Multicellular organisms rely on the movement of signaling molecules across cells, tissues, and organs to communicate among distal sites. In plants, localized leaf damage activates jasmonic acid (JA)-dependent transcriptional reprogramming in both harmed and unharmed tissues. Although it has been indicated that JA species can translocate from damaged into distal sites, the identity of the mobile compound(s), the tissues through which they translocate, and the effect of their relocation remain unknown. Here, we found that following shoot wounding, the relocation of endogenous jasmonates through the phloem is essential to initiate JA signaling and stunt growth in unharmed roots of Arabidopsis thaliana. By employing grafting experiments and hormone profiling, we uncovered that the hormone precursor cis-12-oxo-phytodienoic acid (OPDA) and its derivatives, but not the bioactive JA-Ile conjugate, translocate from wounded shoots into undamaged roots. Upon root relocation, the mobile precursors cooperatively regulated JA responses through their conversion into JA-Ile and JA signaling activation. Collectively, our findings demonstrate the existence of long-distance translocation of endogenous OPDA and its derivatives, which serve as mobile molecules to coordinate shoot-to-root responses, and highlight the importance of a controlled redistribution of hormone precursors among organs during plant stress acclimation.} } @Article{IPB-831, author = {Schilling, S. and Stenzel, I. and von Bohlen, A. and Wermann, M. and Schulz, K. and Demuth, H.-U. and Wasternack, C.}, title = {{Isolation and characterization of the glutaminyl cyclases from Solanum tuberosum and Arabidopsis thaliana: implications for physiological functions}}, year = {2007}, pages = {145-153}, journal = {Biol. Chem}, doi = {10.1515/BC.2007.016}, volume = {388}, } @Article{IPB-388, author = {Feussner, I. and Fritz, I.G. and Hause, B. and Ullrich, W.R. and Wasternack, C.}, title = {{Induction of a new lipoxygenase form in cucumber leaves by salicylic acid or 2,6-dichloroisonicotinic acid}}, year = {1997}, pages = {101-108}, journal = {Bot. Acta}, doi = {10.1111/j.1438-8677.1997.tb00616.x}, url = {http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1438-8677/issues}, volume = {110}, abstract = {Changes in lipoxygenase (LOX) protein pattern and/or activity were investigated in relation to acquired resistance of cucumber (Cucumis sativus L.) leaves against two powdery mildews, Sphaerotheca fuliginea (Schlecht) Salmon and Erysiphe cichoracearum DC et Merat. Acquired resistance was established by spraying leaves with salicylic acid (SA) or 2,6-dichloroisonicotinic acid (INA) and estimated in whole plants by infested leaf area compared to control plants. SA was more effective than INA. According to Western blots, untreated cucumber leaves contained a 97 kDa LOX form, which remained unchanged for up to 48 h after pathogen inoculation. Upon treatment with SA alone for 24 h or with INA plus pathogen, an additional 95 kDa LOX form appeared which had an isoelectric point in the alkaline range. For the induction of this form, a threshold concentration of 1 mM SA was required, higher SA concentrations did not change LOX-95 expression which remained similar between 24 h and 96 h but further increased upon mildew inoculation. Phloem exudates contained only the LOX-97 form, in intercellular washing fluid no LOX was detected. dichloroisonicotinic localization revealed LOX protein in the cytosol of the mesophyll cells without differences between the forms.} }