TY - JOUR ID - 1555 TI - Sequence variability in p27 gene of Citrus tristeza virus (CTV) revealed by SSCP analysis JO - Electronic Journal of Biotechnology PY - 1999 SP - 41-50 AU - Gago, S. AU - Costa, N. AU - Semorile, L. AU - Grau, O. VL - 2 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 388 TI - Induction of a new lipoxygenase form in cucumber leaves by salicylic acid or 2,6-dichloroisonicotinic acid JO - Bot. Acta PY - 1997 SP - 101-108 AU - Feussner, I. AU - Fritz, I.G. AU - Hause, B. AU - Ullrich, W.R. AU - Wasternack, C. VL - 110 UR - http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1438-8677/issues DO - 10.1111/j.1438-8677.1997.tb00616.x AB - Changes in lipoxygenase (LOX) protein pattern and/or activity were investigated in relation to acquired resistance of cucumber (Cucumis sativus L.) leaves against two powdery mildews, Sphaerotheca fuliginea (Schlecht) Salmon and Erysiphe cichoracearum DC et Merat. Acquired resistance was established by spraying leaves with salicylic acid (SA) or 2,6-dichloroisonicotinic acid (INA) and estimated in whole plants by infested leaf area compared to control plants. SA was more effective than INA. According to Western blots, untreated cucumber leaves contained a 97 kDa LOX form, which remained unchanged for up to 48 h after pathogen inoculation. Upon treatment with SA alone for 24 h or with INA plus pathogen, an additional 95 kDa LOX form appeared which had an isoelectric point in the alkaline range. For the induction of this form, a threshold concentration of 1 mM SA was required, higher SA concentrations did not change LOX-95 expression which remained similar between 24 h and 96 h but further increased upon mildew inoculation. Phloem exudates contained only the LOX-97 form, in intercellular washing fluid no LOX was detected. dichloroisonicotinic localization revealed LOX protein in the cytosol of the mesophyll cells without differences between the forms. A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER -