TY - JOUR ID - 1903 TI - Predictive value of hypoglycin A and methylencyclopropylacetic acid conjugates in a horse with atypical myopathy in comparison to its cograzing partners JO - Equine Vet Educ PY - 2018 SP - 24-28 AU - Bochnia, M. AU - Scheidemann, W. AU - Ziegler, J. AU - Sander, J. AU - Vollstedt, S. AU - Glatter, M. AU - Janzen, N. AU - Terhardt, M. AU - Zeyner, A. VL - 30 UR - http://onlinelibrary.wiley.com/journal/10.1001/(ISSN)2042-3292 DO - 10.1111/eve.12596 AB - Hypoglycin A (HGA) was detected in blood and urine of a horse suffering from atypical myopathy (AM; Day 2, serum, 8290 μg/l; urine: Day 1, 574, Day 2, 742 μg/l) and in its cograzing partners with a high variability (46–1570 μg/l serum). Over the period of disease, the level of the toxic metabolites (methylencyclopropylacetic acid [MCPA]-conjugates) increased in body fluids of the AM horse (MCPA-carnitine: Day 2, 0.246, Day 3, 0.581 μmol/l serum; MCPA-carnitine: Day 2, 0.621, Day 3, 0.884 μmol/mmol creatinine in urine) and HGA decreased rapidly (Day 3, 2430 μg/l serum). In cograzing horses MCPA-conjugates were not detected. HGA in seeds ranged from 268 to 367 μg/g. Although HGA was present in body fluids of healthy cograzing horses, MCPA-conjugates were not detectable, in contrast to the AM horse. Therefore, increasing concentrations of MCPA-conjugates are supposed to be linked with the onset of AM and both parameters seem to indicate the clinical stage of disease. However, detection of HGA in body fluids of cograzing horses might be a promising step in preventing the disease. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 1304 TI - Phosphate sensing in root development JO - Curr Opin Plant Biol PY - 2011 SP - 303-309 AU - Abel, S. VL - 14 UR - https://dx.doi.org/10.1016/j.pbi.2011.04.007 DO - 10.1016/j.pbi.2011.04.007 AB - Phosphate (Pi) and its anhydrides constitute major nodes in metabolism. Thus, plant performance depends directly on Pi nutrition. Inadequate Pi availability in the rhizosphere is a common challenge to plants, which activate metabolic and developmental responses to maximize Pi usage and acquisition. The sensory mechanisms that monitor environmental Pi and transmit the nutritional signal to adjust root development have increasingly come into focus. Recent transcriptomic analyses and genetic approaches have highlighted complex antagonistic interactions between external Pi and Fe bioavailability and have implicated the stem cell niche as a target of Pi sensing to regulate root meristem activity. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 854 TI - Auxin signaling JO - Curr Opin Plant Biol PY - 2006 SP - 448-453 AU - Quint, M. AU - Gray, W.M. VL - 9 UR - DO - 10.1016/j.pbi.2006.07.006 AB - Auxin regulates a host of plant developmental and physiological processes, including embryogenesis, vascular differentiation, organogenesis, tropic growth, and root and shoot architecture. Genetic and biochemical studies carried out over the past decade have revealed that much of this regulation involves the SCFTIR1/AFB-mediated proteolysis of the Aux/IAA family of transcriptional regulators. With the recent finding that the TRANSPORT INHIBITOR RESPONSE1 (TIR1)/AUXIN SIGNALING F-BOX (AFB) proteins also function as auxin receptors, a potentially complete, and surprisingly simple, signaling pathway from perception to transcriptional response is now before us. However, understanding how this seemingly simple pathway controls the myriad of specific auxin responses remains a daunting challenge, and compelling evidence exists for SCFTIR1/AFB-independent auxin signaling pathways. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 388 TI - Induction of a new lipoxygenase form in cucumber leaves by salicylic acid or 2,6-dichloroisonicotinic acid JO - Bot. Acta PY - 1997 SP - 101-108 AU - Feussner, I. AU - Fritz, I.G. AU - Hause, B. AU - Ullrich, W.R. AU - Wasternack, C. VL - 110 UR - http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1438-8677/issues DO - 10.1111/j.1438-8677.1997.tb00616.x AB - Changes in lipoxygenase (LOX) protein pattern and/or activity were investigated in relation to acquired resistance of cucumber (Cucumis sativus L.) leaves against two powdery mildews, Sphaerotheca fuliginea (Schlecht) Salmon and Erysiphe cichoracearum DC et Merat. Acquired resistance was established by spraying leaves with salicylic acid (SA) or 2,6-dichloroisonicotinic acid (INA) and estimated in whole plants by infested leaf area compared to control plants. SA was more effective than INA. According to Western blots, untreated cucumber leaves contained a 97 kDa LOX form, which remained unchanged for up to 48 h after pathogen inoculation. Upon treatment with SA alone for 24 h or with INA plus pathogen, an additional 95 kDa LOX form appeared which had an isoelectric point in the alkaline range. For the induction of this form, a threshold concentration of 1 mM SA was required, higher SA concentrations did not change LOX-95 expression which remained similar between 24 h and 96 h but further increased upon mildew inoculation. Phloem exudates contained only the LOX-97 form, in intercellular washing fluid no LOX was detected. dichloroisonicotinic localization revealed LOX protein in the cytosol of the mesophyll cells without differences between the forms. A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER -