TY - JOUR ID - 1960 TI - Differential responses of Coffea arabica L. leaves and roots to chemically induced systemic acquired resistance JO - Genome PY - 2006 SP - 1594-1605 AU - De Nardi, B. AU - Dreos, R. AU - Del Terra, L. AU - Martellossi, C. AU - Asquini, E. AU - Tornincasa, P. AU - Gasperini, D. AU - Pacchioni, B. AU - Rathinavelu, R. AU - Pallavicini, A. AU - Graziosi, G. VL - 49 UR - https://dx.doi.org/10.1139/g06-125 DO - 10.1139/g06-125 AB - Coffea arabica is susceptible to several pests and diseases, some of which affect the leaves and roots. Systemic acquired resistance (SAR) is the main defence mechanism activated in plants in response to pathogen attack. Here, we report the effects of benzo(1,2,3)thiadiazole-7-carbothioic acid-s-methyl ester (BTH), a SAR chemical inducer, on the expression profile of C. arabica. Two cDNA libraries were constructed from the mRNA isolated from leaves and embryonic roots to create 1587 nonredundant expressed sequence tags (ESTs). We developed a cDNA microarray containing 1506 ESTs from the leaves and embryonic roots, and 48 NBS-LRR (nucleotide-binding site leucine-rich repeat) gene fragments derived from 2 specific genomic libraries. Competitive hybridization between untreated and BTH-treated leaves resulted in 55 genes that were significantly overexpressed and 16 genes that were significantly underexpressed. In the roots, 37 and 42 genes were over and underexpressed, respectively. A general shift in metabolism from housekeeping to defence occurred in the leaves and roots after BTH treatment. We observed a systemic increase in pathogenesis-related protein synthesis, in the oxidative burst, and in the cell wall strengthening processes. Moreover, responses in the roots and leaves varied significantly. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 354 TI - Continuous spectrometric assays for glutaminyl cyclase activity JO - Analytical Biochemistry PY - 2002 SP - 49-56 AU - Schilling, S. AU - Hoffmann, T. AU - Wermann, M. AU - Heiser, U. AU - Wasternack, C. AU - Demuth, H.-U. VL - 303 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 1555 TI - Sequence variability in p27 gene of Citrus tristeza virus (CTV) revealed by SSCP analysis JO - Electronic Journal of Biotechnology PY - 1999 SP - 41-50 AU - Gago, S. AU - Costa, N. AU - Semorile, L. AU - Grau, O. VL - 2 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 388 TI - Induction of a new lipoxygenase form in cucumber leaves by salicylic acid or 2,6-dichloroisonicotinic acid JO - Bot. Acta PY - 1997 SP - 101-108 AU - Feussner, I. AU - Fritz, I.G. AU - Hause, B. AU - Ullrich, W.R. AU - Wasternack, C. VL - 110 UR - http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1438-8677/issues DO - 10.1111/j.1438-8677.1997.tb00616.x AB - Changes in lipoxygenase (LOX) protein pattern and/or activity were investigated in relation to acquired resistance of cucumber (Cucumis sativus L.) leaves against two powdery mildews, Sphaerotheca fuliginea (Schlecht) Salmon and Erysiphe cichoracearum DC et Merat. Acquired resistance was established by spraying leaves with salicylic acid (SA) or 2,6-dichloroisonicotinic acid (INA) and estimated in whole plants by infested leaf area compared to control plants. SA was more effective than INA. According to Western blots, untreated cucumber leaves contained a 97 kDa LOX form, which remained unchanged for up to 48 h after pathogen inoculation. Upon treatment with SA alone for 24 h or with INA plus pathogen, an additional 95 kDa LOX form appeared which had an isoelectric point in the alkaline range. For the induction of this form, a threshold concentration of 1 mM SA was required, higher SA concentrations did not change LOX-95 expression which remained similar between 24 h and 96 h but further increased upon mildew inoculation. Phloem exudates contained only the LOX-97 form, in intercellular washing fluid no LOX was detected. dichloroisonicotinic localization revealed LOX protein in the cytosol of the mesophyll cells without differences between the forms. A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER -