TY - JOUR ID - 2195 TI - Ambient temperature and genotype differentially affect developmental and phenotypic plasticity in Arabidopsis thaliana JO - BMC Plant Biol PY - 2017 SP - 114 AU - Ibañez, C. AU - Poeschl, Y. AU - Peterson, T. AU - Bellstädt, J. AU - Denk, K. AU - Gogol-Döring, A. AU - Quint, M. AU - Delker, C. VL - 17 UR - https://dx.doi.org/10.1186/s12870-017-1068-5 DO - 10.1186/s12870-017-1068-5 AB - BackgroundGlobal increase in ambient temperatures constitute a significant challenge to wild and cultivated plant species. Forward genetic analyses of individual temperature-responsive traits have resulted in the identification of several signaling and response components. However, a comprehensive knowledge about temperature sensitivity of different developmental stages and the contribution of natural variation is still scarce and fragmented at best.ResultsHere, we systematically analyze thermomorphogenesis throughout a complete life cycle in ten natural Arabidopsis thaliana accessions grown under long day conditions in four different temperatures ranging from 16 to 28 °C. We used Q10, GxE, phenotypic divergence and correlation analyses to assess temperature sensitivity and genotype effects of more than 30 morphometric and developmental traits representing five phenotype classes. We found that genotype and temperature differentially affected plant growth and development with variing strengths. Furthermore, overall correlations among phenotypic temperature responses was relatively low which seems to be caused by differential capacities for temperature adaptations of individual accessions.ConclusionGenotype-specific temperature responses may be attractive targets for future forward genetic approaches and accession-specific thermomorphogenesis maps may aid the assessment of functional relevance of known and novel regulatory components. A2 - C1 - Molecular Signal Processing ER - TY - CHAP ID - 1935 TI - Radioligand Binding Assays for Determining Dissociation Constants of Phytohormone Receptors T2 - Plant Proteostasis PB - Meth. Mol. Biol PY - 2016 SP - 23-34 AU - Hellmuth, A. AU - Calderón Villalobos, L. I. A. VL - 1450 UR - http://link.springer.com/book/10.1007/978-1-4939-3759-2 SN - 978-1-4939-3757-8 DO - 10.1007/978-1-4939-3759-2_3 AB - In receptor–ligand interactions, dissociation constants provide a key parameter for characterizing binding. Here, we describe filter-based radioligand binding assays at equilibrium, either varying ligand concentrations up to receptor saturation or outcompeting ligand from its receptor with increasing concentrations of ligand analogue. Using the auxin coreceptor system, we illustrate how to use a saturation binding assay to determine the apparent dissociation constant (K D ′ ) for the formation of a ternary TIR1–auxin–AUX/IAA complex. Also, we show how to determine the inhibitory constant (K i) for auxin binding by the coreceptor complex via a competition binding assay. These assays can be applied broadly to characterize a one-site binding reaction of a hormone to its receptor. A2 - Lois, L. M.; Matthiesen, R. C1 - Molecular Signal Processing ER - TY - JOUR ID - 831 TI - Isolation and characterization of the glutaminyl cyclases from Solanum tuberosum and Arabidopsis thaliana: implications for physiological functions JO - Biol. Chem PY - 2007 SP - 145-153 AU - Schilling, S. AU - Stenzel, I. AU - von Bohlen, A. AU - Wermann, M. AU - Schulz, K. AU - Demuth, H.-U. AU - Wasternack, C. VL - 388 UR - AB - A2 - C1 - Molecular Signal Processing ER -