@Article{IPB-2158, author = {Bagchi, R. and Melnyk, C. W. and Christ, G. and Winkler, M. and Kirchsteiner, K. and Salehin, M. and Mergner, J. and Niemeyer, M. and Schwechheimer, C. and Calderón Villalobos, L. I. A. and Estelle, M.}, title = {{The Arabidopsis ALF4 protein is a regulator of SCF E3 ligases.}}, year = {2018}, pages = {255-268}, journal = {EMBO J}, doi = {10.15252/embj.201797159}, url = {http://onlinelibrary.wiley.com/doi/10.15252/embj.201797159/full}, volume = {37}, abstract = {The cullin-RING E3 ligases (CRLs) regulate diverse cellular processes in all eukaryotes. CRL activity is controlled by several proteins or protein complexes, including NEDD8, CAND1, and the CSN. Recently, a mammalian protein called Glomulin (GLMN) was shown to inhibit CRLs by binding to the RING BOX (RBX1) subunit and preventing binding to the ubiquitin-conjugating enzyme. Here, we show that Arabidopsis ABERRANT LATERAL ROOT FORMATION4 (ALF4) is an ortholog of GLMN. The alf4 mutant exhibits a phenotype that suggests defects in plant hormone response. We show that ALF4 binds to RBX1 and inhibits the activity of SCFTIR1, an E3 ligase responsible for degradation of the Aux/IAA transcriptional repressors. In vivo, the alf4 mutation destabilizes the CUL1 subunit of the SCF. Reduced CUL1 levels are associated with increased levels of the Aux/IAA proteins as well as the DELLA repressors, substrate of SCFSLY1. We propose that the alf4 phenotype is partly due to increased levels of the Aux/IAA and DELLA proteins.} } @Article{IPB-831, author = {Schilling, S. and Stenzel, I. and von Bohlen, A. and Wermann, M. and Schulz, K. and Demuth, H.-U. and Wasternack, C.}, title = {{Isolation and characterization of the glutaminyl cyclases from Solanum tuberosum and Arabidopsis thaliana: implications for physiological functions}}, year = {2007}, pages = {145-153}, journal = {Biol. Chem}, doi = {10.1515/BC.2007.016}, volume = {388}, }